LOCUS       sequence-387536-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-3 u3B 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u3B
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..28
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        57..458
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             504..524
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             540..881
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             894..920
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             900..926
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2609..2796
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2664..2688)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2817..2850)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2928..2961)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3005..3038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3116..3149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3193..3226)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3304..3337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3846..4940)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4941..5346)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5823..7165
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6786..6833)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7430..7534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7535..7603,7604..8395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7753..7772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8566..9154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9055..9074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9308..9325
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9340..9480
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9406..9425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
       61 gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc
      121 aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa
      181 cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt
      241 ccttcattca cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac
      301 ttgaatttga aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat
      361 tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct
      421 gcacaatatt tcaagctata ccaagcatac aatcaactcc aagctttgca aagatggata
      481 aagcggaatt aattcccgag cctccaaaaa agaagagaaa ggtcgaattg ggtaccgccg
      541 ccaattttaa tcaaagtggg aatattgctg atagctcatt gtccttcact ttcactaaca
      601 gtagcaacgg tccgaacctc ataacaactc aaacaaattc tcaagcgctt tcacaaccaa
      661 ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct agtaaaattg
      721 atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact gcgtataacg
      781 cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta tataactatc
      841 tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gatctctatg gcttacccat
      901 acgatgttcc agattacgct agcttgggtg gtcatatggc catggaggcc ccggggatcc
      961 ccaccatgat tcacagtctt ttcttgatca actctgctgg ggatattttc ctggagaagc
     1021 actggaaaag cgtggtcagc cgctctgttt gcgattactt tttcgaggcc caagagagag
     1081 ctacggaggc tgaaaatgtg cctcccgtca tccccacacc tcaccactac cttctgagcg
     1141 tttaccggca caagatcttc tttgtggctg tgatccagac ggaggtcccg cctctgtttg
     1201 tcattgaatt ccttcaccga gtagtggaca catttcagga ctatttcggg gtctgctctg
     1261 agccagtgat caaagacaac gtggtggtgg tctacgaggt gttggaagag atgctggaca
     1321 atgggttccc cttggctacg gagtcgaaca tcctcaagga gctgataaag cctcccacca
     1381 ttctccggac agtagtcaac accatcacag gaagcaccaa tgtgggcgac cagcttccaa
     1441 ctgggcagct gtcggtggtg ccttggcgac ggactggggt gaaatatacc aacaacgagg
     1501 cctatttcga cgtggttgaa gagatagacg ccatcattga caaatcaggt tccacagtca
     1561 ccgctgagat ccaaggggtg attgatgcct gtgttaaact gaccgggatg ccagatctca
     1621 cgctgtcctt catgaacccc aggttgctgg atgatgttag cttccacccc tgtgtccgtt
     1681 tcaagcgctg ggaatctgag cgcatcctct ccttcatccc tcctgacggc aacttccgcc
     1741 tgctctccta ccacgtcagt gcacagaacc tagttgccat tccagtctat gtcaaacaca
     1801 gcatcagctt ccgggacagt ggctcacttg gacgctttga aatcacagtg gggccgaagc
     1861 agaccatggg gaagaccata gagggtgtga ctgtaaccag ccagatgccc aaaggagtct
     1921 tgaacatgag cctcactcca tcccagggga cacacacgtt tgacccggtg acaaagatgc
     1981 tgtcttggga tgtaggaaaa ataaacccgc aaaagctgcc aagtttgaag gggacaatgg
     2041 gtctccaggt cggagcttct aaacccgatg agaaccctac aattaacctt cagttcaaga
     2101 tccagcagct ggccatttct ggactcaaag tgaaccgtct ggatatgtac ggagagaagt
     2161 acaagccctt taagggcata aaatacatga ccaaagccgg caagttccaa gtccgaacct
     2221 gagtcgagag atctatgaat cgtagatact gaaaaacccc gcaagttcac ttcaactgtg
     2281 catcgtgcac catctcaatt tctttcattt atacatcgtt ttgccttctt ttatgtaact
     2341 atactcctct aagtttcaat cttggccatg taacctctga tctatagaat tttttaaatg
     2401 actagaatta atgcccatct tttttttgga cctaaattct tcatgaaaat atattacgag
     2461 ggcttattca gaagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt
     2521 tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg
     2581 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat
     2641 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     2701 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     2761 gcatgaggtc gctcttattg accacacctc taccggcatg ccggtcgagg gacctaataa
     2821 cttcgtatag catacattat acgaagttat attaagggtt ccggatcgcg gccgctcgac
     2881 ctgcagccaa gctagcttgg ctggacgtaa actcctcttc agacctaata acttcgtata
     2941 gcatacatta tacgaagtta tattaagggt tattgaatat gatcggaatt ggtcgaggga
     3001 cctaataact tcgtatagca tacattatac gaagttatat taagggttcc ggatcgcggc
     3061 cgctcgacct gcagccaagc tagcttggct ggacgtaaac tcctcttcag acctaataac
     3121 ttcgtatagc atacattata cgaagttata ttaagggtta ttgaatatga tcggaattgg
     3181 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccgg
     3241 atcgcggccg ctcgacctgc agccaagcta gcttggctgg acgtaaactc ctcttcagac
     3301 ctaataactt cgtatagcat acattatacg aagttatatt aagggttatt gaatatgatc
     3361 ggaattggtc gactacgtcg ttaaggccgt ttctgacaga gtaaaattct tgagggaact
     3421 ttcaccatta tgggaaatgg ttcaagaagg tattgactta aactccatca aatggtcagg
     3481 tcattgagtg ttttttattt gttgtatttt ttttttttta gagaaaatcc tccaatatat
     3541 aaattaggaa tcatagtttc atgattttct gttacaccta actttttgtg tggtgccctc
     3601 ctccttgtca atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag
     3661 tatcaatttg cttacctgta ttcctttaca tcctcctttt tctccttctt gataaatgta
     3721 tgtagattgc gtatatagtt tcgtctaccc tatgaacata ttccattttg taatttcgtg
     3781 tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa
     3841 tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac ttcggtggta
     3901 ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca
     3961 tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca
     4021 gacaagatag tggcgatagg gttgacctta ttctttggca aatctggagc agaaccgtgg
     4081 catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat
     4141 ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac
     4201 atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg
     4261 gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa attcgttctt gatggtttcc
     4321 tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa
     4381 ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc
     4441 acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt
     4501 ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta
     4561 gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt
     4621 cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta
     4681 acactacctg taccccattt aggaccaccc acagcaccta acaaaacggc atcagccttc
     4741 ttggaggctt ccagcgcctc atctggaagt ggaacacctg tagcatcgat agcagcacca
     4801 ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta
     4861 agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg
     4921 atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat atattgctga
     4981 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     5041 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     5101 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     5161 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     5221 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     5281 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     5341 acagttcctt accactcttt tgttactcta ttgatccagc tcagcaaagg cagtgtgatc
     5401 taagattcta tcttcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc
     5461 aaaaggcact tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca
     5521 atgatattcg aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga
     5581 tttacgatcg tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc
     5641 ctgaaacaga tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag
     5701 acaatgtatg tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc
     5761 acgtcgcatc cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg
     5821 ttaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt
     5881 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat
     5941 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct
     6001 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc
     6061 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga
     6121 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa
     6181 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt
     6241 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac
     6301 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg
     6361 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc
     6421 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta
     6481 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta
     6541 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa
     6601 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga
     6661 gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag
     6721 tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc
     6781 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt
     6841 ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc
     6901 acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag
     6961 tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt
     7021 ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac
     7081 taccctttag ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg
     7141 ctatcatttc ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt
     7201 gatcaggtat tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct
     7261 tatcgctcca atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg
     7321 aggtcatcaa atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat
     7381 aaggcgcatt tttcttcaaa gctcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7501 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7561 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7621 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7681 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7741 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     7801 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7861 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7921 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7981 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8041 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     8101 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8161 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8221 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8281 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8341 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8401 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9181 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9241 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9301 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     9361 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     9421 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     9481 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9541 gcttacagac aagctgtgac
//
