LOCUS       sequence-387537-        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-3 u3A 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u3A
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..30
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        59..460
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             506..526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             542..883
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             896..922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             902..928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2614..2801
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2669..2693)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(2822..2855)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2933..2966)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3010..3043)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3121..3154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3198..3231)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3309..3342)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3851..4945)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4946..5351)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5828..7170
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6791..6838)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7435..7539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7540..7608,7609..8400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7758..7777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8571..9159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9060..9079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9313..9330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9345..9485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9411..9430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
       61 cagcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc
      121 gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc
      181 aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc
      241 ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt
      301 acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca
      361 attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt
      421 ctgcacaata tttcaagcta taccaagcat acaatcaact ccaagctttg caaagatgga
      481 taaagcggaa ttaattcccg agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc
      541 cgccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa
      601 cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc
      661 aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat
      721 tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa
      781 cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg tatataacta
      841 tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagatctcta tggcttaccc
      901 atacgatgtt ccagattacg ctagcttggg tggtcatatg gccatggagg ccccggggat
      961 ccccgaattc atgatccaca gtctatttct catcaactgt tctggcgaca tatttctaga
     1021 aaaacactgg aagagcgttg taagccaatc tgtgtgtgac tatttctttg aagctcagga
     1081 gaaagctgct gatgttgaaa atgtaccacc tgtcatttca acacctcacc actacctcat
     1141 tagtatctac cgggataagc tcttctttgt gtctgtgata cagactgaag tgccacctct
     1201 ctttgtaatt gagtttctgc atcgagttgc tgacactttt caggactact ttggtgagtg
     1261 ttcagaggct gcaattaagg ataatgtggt catagtgtat gagctcttgg aagaaatgtt
     1321 agacaatgga ttcccactgg ctaccgaatc taacattctg aaagaactga ttaaaccacc
     1381 aacaattcta cgttctgtcg tcaattctat tacaggcagt agtaatgttg gggacacgct
     1441 ccccactggg cagctgtcca acatcccatg gcgtcgagca ggtgtaaagt acaccaacaa
     1501 tgaagcctac tttgatgtag tcgaagagat agatgcgatt atagataaat caggatctac
     1561 agtctttgca gaaattcaag gtgtcattga tgcttgcatt aagctgtctg gaatgcctga
     1621 tctctctctc tctttcatga acccaaggct tctagatgat gtcagcttcc acccatgcat
     1681 ccggttcaaa cgctgggaat ctgagagagt tttgtcattc attcctcccg atggaaattt
     1741 ccgactcata tcataccgcg tcagctcaca aaatctagtg gcaatcccag tgtatgtgaa
     1801 acataatatc agctttaagg aaaacagctc ttgtggtaga tttgatataa caattggacc
     1861 aaaacagaat atgggaaaaa cgattgaagg aatcacagtg actgttcaca tgccaaaagt
     1921 tgtgctgaat atgaacctga caccaacaca aggcagctat acattcgatc cagtcaccaa
     1981 ggtactagca tgggatgtgg ggaaaattac tccacaaaag ctcccaagtc ttaaaggact
     2041 ggtaaattta cagtcaggag cacccaagcc agaagagaac ccaaacctca acatacagtt
     2101 caagatccag cagcttgcta tttcaggctt aaaagtgaac cgcttggaca tgtatggtga
     2161 gaagtataag ccatttaaag gagtcaagta tatcacaaag gccggaaagt tccaagtgag
     2221 gacatgagtc gagagatcta tgaatcgtag atactgaaaa accccgcaag ttcacttcaa
     2281 ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc ttcttttatg
     2341 taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat agaatttttt
     2401 aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg aaaatatatt
     2461 acgagggctt attcagaagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc
     2521 aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa gggtcaaatc
     2581 gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt
     2641 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     2701 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     2761 gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccggt cgagggacct
     2821 aataacttcg tatagcatac attatacgaa gttatattaa gggttccgga tcgcggccgc
     2881 tcgacctgca gccaagctag cttggctgga cgtaaactcc tcttcagacc taataacttc
     2941 gtatagcata cattatacga agttatatta agggttattg aatatgatcg gaattggtcg
     3001 agggacctaa taacttcgta tagcatacat tatacgaagt tatattaagg gttccggatc
     3061 gcggccgctc gacctgcagc caagctagct tggctggacg taaactcctc ttcagaccta
     3121 ataacttcgt atagcataca ttatacgaag ttatattaag ggttattgaa tatgatcgga
     3181 attggtcgag ggacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
     3241 tccggatcgc ggccgctcga cctgcagcca agctagcttg gctggacgta aactcctctt
     3301 cagacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttattgaata
     3361 tgatcggaat tggtcgacta cgtcgttaag gccgtttctg acagagtaaa attcttgagg
     3421 gaactttcac cattatggga aatggttcaa gaaggtattg acttaaactc catcaaatgg
     3481 tcaggtcatt gagtgttttt tatttgttgt attttttttt ttttagagaa aatcctccaa
     3541 tatataaatt aggaatcata gtttcatgat tttctgttac acctaacttt ttgtgtggtg
     3601 ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc acgttgagcc
     3661 attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc ttcttgataa
     3721 atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca ttttgtaatt
     3781 tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa
     3841 gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg
     3901 tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa
     3961 ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg
     4021 cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct ggagcagaac
     4081 cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg
     4141 cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac
     4201 caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca
     4261 tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg
     4321 tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg
     4381 accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc
     4441 tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt
     4501 cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac
     4561 ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac
     4621 atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag
     4681 gtctaacact acctgtaccc catttaggac cacccacagc acctaacaaa acggcatcag
     4741 ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca tcgatagcag
     4801 caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag
     4861 ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa
     4921 cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata tatatatatt
     4981 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
     5041 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
     5101 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt
     5161 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
     5221 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
     5281 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
     5341 ttcccacagt tccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg
     5401 tgatctaaga ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga
     5461 aatgcaaaag gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct
     5521 tctcaatgat attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt
     5581 acagatttac gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt
     5641 tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac
     5701 ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat
     5761 cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat
     5821 ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     5881 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     5941 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     6001 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     6061 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     6121 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     6181 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     6241 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     6301 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     6361 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     6421 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     6481 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     6541 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     6601 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     6661 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     6721 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     6781 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa
     6841 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
     6901 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
     6961 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag
     7021 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc
     7081 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt
     7141 caatgctatc atttcctttg atattggatc atatgcatag taccgagaaa ctagtgcgaa
     7201 gtagtgatca ggtattgctg ttatctgatg agtatacgtt gtcctggcca cggcagaagc
     7261 acgcttatcg ctccaatttc ccacaacatt agtcaactcc gttaggccct tcattgaaag
     7321 aaatgaggtc atcaaatgtc ttccaatgtg agattttggg ccatttttta tagcaaagat
     7381 tgaataaggc gcatttttct tcaaagctca ggtggcactt ttcggggaaa tgtgcgcgga
     7441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7501 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7561 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7621 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7681 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7741 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     7801 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7861 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7921 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7981 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8041 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     8101 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8161 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8221 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8281 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8341 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8701 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     8761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9181 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9241 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9301 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     9361 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     9421 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     9481 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9541 catccgctta cagacaagct gtg
//
