LOCUS       sequence-387540-        9678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-4 u4 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u4
FEATURES             Location/Qualifiers
     source          1..9678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..46
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        75..476
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             522..542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             558..899
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             918..944
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     terminator      2933..3120
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2988..3012)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(3141..3174)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3252..3285)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3329..3362)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(3440..3473)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3982..5076)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5077..5482)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      5959..7301
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6922..6969)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7566..7670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7671..7739,7740..8531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7889..7908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8702..9290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9191..9210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9444..9461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9476..9616
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9542..9561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
       61 accgaaacgc gcgaggcagc aacttctttt cttttttttt cttttctctc tcccccgttg
      121 ttgtctcacc atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa
      181 cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc
      241 acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac
      301 ggccttcctt ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag
      361 tataaataga cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc
      421 ttccttgttt ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa
      481 gctttgcaaa gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg
      541 tcgaattggg taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt
      601 ccttcacttt cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc
      661 aagcgctttc acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa
      721 tcacggctag taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg
      781 accaaactgc gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg
      841 atgatgtata taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga
      901 tctctatggc ttacccatac gatgttccag attacgctag cttgggtggt catatggcca
      961 tggaggcccc ggggatccga ggagatgcca ccatgatttc ccaattcttc attctgtcct
     1021 ccaaggggga cccgctcatc tacaaagact tccgcgggga cagtggcggc cgggatgtgg
     1081 ccgagctctt ctaccggaag ctgacgggac tgccaggaga cgagtccccg gttgtcatgc
     1141 atcaccatgg ccgtcatttc attcacatca gacacagcgg cctctatttg gtggtcacaa
     1201 cttcagaaaa cgtttctccc ttcagcctcc tagaactgct ctccaggttg gccacccttc
     1261 tgggcgatta ctgtggctcc ctgggcgagg ggaccatctc ccgcaatgtg gctctggtat
     1321 acgaactcct ggatgaagtg ctggactatg gctatgtaca gaccacatcc acggagatgc
     1381 tgaggaattt catccagacg gaagctgtgg tcagcaagcc cttcagcctc tttgacctca
     1441 gcagcgttgg cttgtttggg gctgagacac aacagagcaa agtggccccc agcagtgcag
     1501 ccagccgccc cgtcctgtcc agtcgctctg accagagcca aaagaatgaa gtttttttgg
     1561 atgtggtcga gagattgtct gtactgatag catctaatgg atccctgctg aaggtggatg
     1621 tgcagggaga gattcggctc aagagcttcc ttcctagcgg ctctgagatg cgcattggct
     1681 tgacggaaga gttttgtgtg gggaagtcag agctgagagg ttatgggcca ggaatccggg
     1741 tcgatgaagt ctcgtttcac agctctgtga atctggacga atttgagtct catcgaatcc
     1801 tccgcttgca accacctcag ggcgagctga ctgtgatgcg gtaccaactc tccgatgacc
     1861 tcccctcacc gctccccttc cggctcttcc cctctgtgca gtgggaccga ggctcaggcc
     1921 ggctccaggt ttatctaaag ttgcgatgtg acctgctctc aaagagccaa gccctcaatg
     1981 tcaggctgca cctccccctg cctcgagggg tggtcagcct gtctcaggag ctgagcagcc
     2041 cagagcagaa ggctgagctg gcagagggag cccttcgctg ggacctgcct cgggtgcaag
     2101 gaggctctca actctcaggc cttttccaga tggacgtccc agggccccca ggacctccca
     2161 gccatgggct ctccacctcg gcctctcctc tggggctggg ccctgccagt ctctccttcg
     2221 agcttccccg gcacacgtgc tctggcctcc aggtccgatt cctcaggctg gccttcaggc
     2281 catgcggcaa tgccaacccc cacaagtggg tgcgacacct aagccacagc gacgcctatg
     2341 tcattcggat ctgaggctcc ccaaacgagg acacgacggc caaggtggca gtttgtccca
     2401 cgggaggaca gtcgtttctt ttccagcctc ctggccttcg gactctgaat ctgggcagga
     2461 agagtcctca gtcccaagac caggaggggg caatgggccc agcctttctg tggtatctga
     2521 tgcaggaagg actgcagaac caatgcctcg agagatctat gaatcgtaga tactgaaaaa
     2581 ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc atttatacat
     2641 cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc catgtaacct
     2701 ctgatctata gaatttttta aatgactaga attaatgccc atcttttttt tggacctaaa
     2761 ttcttcatga aaatatatta cgagggctta ttcagaagct ttggacttct tcgccagagg
     2821 tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa
     2881 gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt
     2941 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     3001 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     3061 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     3121 catgccggtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag
     3181 ggttccggat cgcggccgct cgacctgcag ccaagctagc ttggctggac gtaaactcct
     3241 cttcagacct aataacttcg tatagcatac attatacgaa gttatattaa gggttattga
     3301 atatgatcgg aattggtcga gggacctaat aacttcgtat agcatacatt atacgaagtt
     3361 atattaaggg ttccggatcg cggccgctcg acctgcagcc aagctagctt ggctggacgt
     3421 aaactcctct tcagacctaa taacttcgta tagcatacat tatacgaagt tatattaagg
     3481 gttattgaat atgatcggaa ttggtcgact acgtcgttaa ggccgtttct gacagagtaa
     3541 aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt gacttaaact
     3601 ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt tttttagaga
     3661 aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta cacctaactt
     3721 tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct ttttccttat
     3781 cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct cctttttctc
     3841 cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg aacatattcc
     3901 attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata
     3961 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc
     4021 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa
     4081 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt
     4141 caacatcatt gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc
     4201 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga
     4261 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga
     4321 gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt
     4381 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg taggaaattc
     4441 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc
     4501 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat
     4561 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc
     4621 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac
     4681 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga
     4741 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa
     4801 accttgttca ggtctaacac tacctgtacc ccatttagga ccacccacag cacctaacaa
     4861 aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc
     4921 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac
     4981 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
     5041 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat
     5101 atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac gattgctaac
     5161 cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg
     5221 caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcgctctca
     5281 cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga
     5341 aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt
     5401 ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg
     5461 atacctgagt attcccacag ttccttacca ctcttttgtt actctattga tccagctcag
     5521 caaaggcagt gtgatctaag attctatctt cgcgatgtag taaaactagc tagaccgaga
     5581 aagagactag aaatgcaaaa ggcacttcta caatggctgc catcattatt atccgatgtg
     5641 acgctgcagc ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga
     5701 caaactgttt tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg
     5761 aacctgggag ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc
     5821 tagcgcttta cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt
     5881 gcataggtaa tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt
     5941 caatagcata tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac
     6001 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     6061 aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat
     6121 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     6181 aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca
     6241 aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc
     6301 tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt
     6361 agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc
     6421 cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg
     6481 attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga
     6541 tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac
     6601 gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact
     6661 acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt
     6721 ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag
     6781 agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt
     6841 tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct
     6901 tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
     6961 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
     7021 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
     7081 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
     7141 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
     7201 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
     7261 gtctcatcct tcaatgctat catttccttt gatattggat catatgcata gtaccgagaa
     7321 actagtgcga agtagtgatc aggtattgct gttatctgat gagtatacgt tgtcctggcc
     7381 acggcagaag cacgcttatc gctccaattt cccacaacat tagtcaactc cgttaggccc
     7441 ttcattgaaa gaaatgaggt catcaaatgt cttccaatgt gagattttgg gccatttttt
     7501 atagcaaaga ttgaataagg cgcatttttc ttcaaagctc aggtggcact tttcggggaa
     7561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     7921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     8221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8821 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     8881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     9481 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     9541 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     9601 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9661 tctgctcccg gcatccgc
//
