LOCUS       sequence-38781-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1281
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg aagttcctat accttttgaa gaataggaac
       61 ttcggaatag gaacttcggc gcgccttctg tgcctctaaa gagagagaat cacctcagtt
      121 cttccagcca aagatgtcac cagcaaggta attctgattg tctagcagta attttcaatg
      181 gctcttgcag agtttgtaaa gagtaatttt catcttgtag aaagagttaa attgcaagat
      241 gatacattca aaaatcagta tttctctcag tgacaggtgc ttgaataaaa attagaatcc
      301 ttttcacctg ctgtctgggc acatggttgg ccccagtatc tattgaatgc catacttcta
      361 tagtgctgga aataaccctg gggacaatct tttcataaca gggatgtacc actcaagctt
      421 agtgtatttt ttgacgaagg aaacagcttc aagacattga ttttcttctg ctttaaaatt
      481 tcagtatctg actaatataa gactagagta tcatctattt aataattatt ctgctcgtgt
      541 gtgtgcaaat gtataaatat aatgtgtata tatacatata attttaaaaa agattttgta
      601 tatacattac tttttcctct ttcaattgta gatattacgg aaacttattt ccttacacct
      661 ggatttgaac ttcttaactc agtagtgaat attaatactc aaaatattac tgaatcatat
      721 gttgtcattg aaataaattt ttccataaga ggaaaatata tattaaaagc aaagcttgca
      781 tttcttgcat ttaatcaact tacacatttc atttctag
//
