LOCUS       sequence-38782-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: secretedBirA-8his
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..228
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    227..282
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(354..377)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
       61 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
      121 atgtcgtagt caggtttagt tcgtccgggg cggggatcga tcctctagct agagatctcc
      181 gagggatctc gaccaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
      241 aattccaaca cactattgca atgaaaataa atttccttta ttagccagag gtcgaggtcg
      301 ggggatccgg ccttgccggc ctcgagcggc cgcccgggtc gactctagac tcagtggtgg
      361 tggtggtggt ggtggtgctc gagtttttct gcactacgca gggatatttc accgcccatc
      421 cagggtttta ttattccatc ctgctcaagt aataaagccc cctgtttgtc tattccgcgt
      481 gaaatgccaa atatttcttt atcaccaatg ataagtttca ctgggcgatt aataaaatta
      541 tccagctttt cccagcgcga cagataaggt gccaatcctt cttgttcgaa gagttccaac
      601 gcagcacgta attcacgtat tagcatggcc gccaacgtat tacgatcgag attgatcccc
      661 gcttcctgca gcgtgatcca cccctgatta acgacactct cttcaacacg gcgcattgcc
      721 atgttgatcc cggctccaat gactatttgc gccgcatcac cagttttgcc agtcagctcc
      781 accagaatgc ctgccagctt gcgatcctgc agatagaggt cattaggcca tttaacacga
      841 acttt
//
