LOCUS       sequence-38783-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1307
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(158..176)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        255..359
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(578..597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcggaagtt cctatacctt ttgaagaata ggaacttcgg
       61 aataggaact tcggcgcgcc acgcgtggcc ggcctaggag ctcgaattca ttaagggtta
      121 ttgaatatga tcggaattaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      181 taggttaatg tcatgataat aatggtttct tagacgtgcg gccgccgtca ggtggcactt
      241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      601 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      901 ctg
//
