LOCUS       sequence-387831-       10221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-4 
            beta-4 fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Mattera R, De Pace R, Bonifacino JS
  TITLE     The adaptor protein chaperone AAGAB stabilizes AP-4 complex 
            subunits.
  JOURNAL   Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17.
  PUBMED    35976721
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-10-1"; volume: "33(12):ar109. doi"; pages: " 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17"
COMMENT     Sequence Label: pGADT7-AP-4 beta-4
FEATURES             Location/Qualifiers
     source          1..10221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        763..1467
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1513..1533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1549..1890
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1896..1915
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1896..1914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1921..1930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1933..1959
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1996..2010
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     terminator      4641..4828
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4696..4720)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4945..6039)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6040..6445)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6487..6503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6487..6503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6500..6522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6511..6527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6535..6541,6542..6559,6560..6565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6580..6601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6656..6689
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6767..6800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6869..6886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7040..7628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7120..7139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7799..8590,8591..8659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8422..8441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8660..8764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8832..8850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8888..8910)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9010..9029
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      9046..10210
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcaggtcga gatccgggat cgaagaaatg atggtaaatg aaataggaaa tcaaggagca
       61 tgaaggcaaa agacaaatat aagggtcgaa cgaaaaataa agtgaaaagt gttgatatga
      121 tgtatttggc tttgcggcgc cgaaaaaacg agtttacgca attgcacaat catgctgact
      181 ctgtggcgga cccgcgctct tgccggcccg gcgataacgc tgggcgtgag gctgtgcccg
      241 gcggagtttt ttgcgcctgc attttccaag gtttaccctg cgctaagggg cgagattgga
      301 gaagcaataa gaatgccggt tggggttgcg atgatgacga ccacgacaac tggtgtcatt
      361 atttaagttg ccgaaagaac ctgagtgcat ttgcaacatg agtatactag aagaatgagc
      421 caagacttgc gagacgcgag tttgccggtg gtgcgaacaa tagagcgacc atgaccttga
      481 aggtgagacg cgcataaccg ctagagtact ttgaagagga aacagcaata gggttgctac
      541 cagtataaat agacaggtac atacaacact ggaaatggtt gtctgtttga gtacgctttc
      601 aattcatttg ggtgtgcact ttattatgtt acaatatgga agggaacttt acacttctcc
      661 tatgcacata tattaattaa agtccaatgc tagtagagaa ggggggtaac acccctccgc
      721 gctcttttcc gatttttttc taaaccgtgg aatatttcgg atatcctttt gttgtttccg
      781 ggtgtacaat atggacttcc tcttttctgg caaccaaacc catacatcgg gattcctata
      841 ataccttcgt tggtctccct aacatgtagg tggcggaggg gagatataca atagaacaga
      901 taccagacaa gacataatgg gctaaacaag actacaccaa ttacactgcc tcattgatgg
      961 tggtacataa cgaactaata ctgtagccct agacttgata gccatcatca tatcgaagtt
     1021 tcactaccct ttttccattt gccatctatt gaagtaataa taggcgcatg caacttcttt
     1081 tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca atgacaaaaa
     1141 aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt
     1201 gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc cttcattcac
     1261 gcacactact ctctaatgag caacggtata cggccttcct tccagttact tgaatttgaa
     1321 ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt attaatcttt
     1381 tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg cacaatattt
     1441 caagctatac caagcataca atcaactcca agctttgcaa agatggataa agcggaatta
     1501 attcccgagc ctccaaaaaa gaagagaaag gtcgaattgg gtaccgccgc caattttaat
     1561 caaagtggga atattgctga tagctcattg tccttcactt tcactaacag tagcaacggt
     1621 ccgaacctca taacaactca aacaaattct caagcgcttt cacaaccaat tgcctcctct
     1681 aacgttcatg ataacttcat gaataatgaa atcacggcta gtaaaattga tgatggtaat
     1741 aattcaaaac cactgtcacc tggttggacg gaccaaactg cgtataacgc gtttggaatc
     1801 actacaggga tgtttaatac cactacaatg gatgatgtat ataactatct attcgatgat
     1861 gaagataccc caccaaaccc aaaaaaagag atctttaata cgactcacta tagggcgagc
     1921 gccgccatgg agtacccata cgacgtacca gattacgctc atatggccat ggaggccagt
     1981 gaattccacc cgggcgacga cgacgacaag atgccgtacc ttggctccga ggacgtggtg
     2041 aaggagctga agaaggctct gtgcaatcct cacattcaag ctgataggct gcgctaccgg
     2101 aatgtcatcc agcgagtgat taggtacatg actcaaggct tggacatgtc tggtgttttt
     2161 atggaaatgg tgaaggccag tgccactgta gatattgtcc agaagaagtt ggtttatctg
     2221 tacatgtgca catatgctcc cctgaaacca gatctggctc tcctggccat caatacgctg
     2281 tgcaaagact gctcagaccc caatccaatg gtgcgagggc tggcgttacg gagcatgtgt
     2341 agcctcagga tgcctggtgt gcaggagtat atacaacagc ctattctcaa tggtctgcgg
     2401 gataaggctt catatgtcag gagagtggta gtccttggat gtgccaagat gcataatctt
     2461 catggagact ctgaagtaga tggtgccctg gtaaatgaat tatacagttt gctgcgtgac
     2521 caggatccaa ttgtagttgt gaactgcttg aggtctctag aggaaattct gaaacaggaa
     2581 ggaggcgttg tcatcaataa gcccattgct caccatctct taaatcgaat gtcaaaactg
     2641 gaccaatggg gccaggctga agtattgaac tttctgctac gctaccaacc ccgcagtgag
     2701 gaagaactat ttgacattct caatctgttg gatagtttcc tcaagagcag tagcccaggt
     2761 gtggtgatgg gagctaccaa actttttctg atcttggcaa aaatgtttcc ccacgtacaa
     2821 actgatgtcc ttgtgcgggt caagggacct ttgctagctg cctgttcttc agagagccgt
     2881 gagctctgtt ttgttgctct ttgtcatgta cgccagatct tgcatagttt accaggtcac
     2941 tttagcagcc actacaaaaa gtttttttgc tcctactcgg agccccacta catcaaacta
     3001 cagaaagtgg aggtgctgtg tgaactggtg aacgatgaga atgtgcagca ggtgctagag
     3061 gagcttcgag ggtactgcac ggatgtgtct gcggactttg cacaggctgc catctttgcc
     3121 ataggtggca ttgccaggac ttacacagat caatgtgttc agattttaac agagttgctg
     3181 ggtcttcgac aagagcacat taccacagtg gtggtgcaga ctttccgaga cctggtttgg
     3241 ttgtgtcctc agtgtactga agctgtatgt caggccctgc ccggctgtga agagaacatt
     3301 caagatagtg aggggaagca agcacttatt tggctacttg gtgtccatgg ggaaagaatt
     3361 cctaatgctc cttatgtgtt agaggacttt gttgagaatg tgaagtcgga aacatttcca
     3421 gctgttaaga tggagctgct cactgcttcg ctgcgccttt tcctctcccg acctgctgag
     3481 tgccaggaca tgctaggacg tttgttgtat tactgcatag aggaagaaaa agatatggct
     3541 gtacgggacc gaggtctctt ctattatcgc ctcctcttag ttggcattga tgaagttaag
     3601 cggattctgt gtagccctaa atctgaccct actcttggac ttttggagga tccggcagaa
     3661 agacctgtga atagctgggc ctcagacttc aacacactgg tgccagtgta tggcaaagcc
     3721 cactgggcaa ctatctctaa atgccagggg gcagagcgtt gtgacccaga gcttcctaaa
     3781 acttcatcct ttgccgcatc aggacccttg attcctgaag agaacaagga gagggtacaa
     3841 gaactccctg attctggagc cctcatgcta gtccccaatc gccagcttac tgctgattat
     3901 tttgagaaaa cttggcttag ccttaaagtt gctcatcagc aagtgttgcc ttggcgggga
     3961 gaattccatc ctgacaccct ccagatggct cttcaagtag tgaacatcca gaccatcgca
     4021 atgagtaggg ctgggtctcg gccatggaaa gcatacctca gtgctcagga tgatactggc
     4081 tgtctgttct taacagaact gctattggag cctggaaact cagaaatgca gatctctgtg
     4141 aaacaaaatg aagcaagaac ggagacgctg aatagtttta tttctgtatt agaaactgtg
     4201 attggaacaa ttgaagaaat aaaatcataa cctgcagcca agcttgcggc cgcactcgag
     4261 ctgcagatga atcgtagata ctgaaaaacc ccgcaagttc acttcaactg tgcatcgtgc
     4321 accatctcaa tttctttcat ttatacatcg ttttgccttc ttttatgtaa ctatactcct
     4381 ctaagtttca atcttggcca tgtaacctct gatctataga attttttaaa tgactagaat
     4441 taatgcccat cttttttttg gacctaaatt cttcatgaaa atatattacg agggcttatt
     4501 cagaagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag gttgtcggct
     4561 tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg
     4621 ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
     4681 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     4741 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     4801 tcgctcttat tgaccacacc tctaccggcc ggtcgaaatt cccctaccct atgaacatat
     4861 tccattttgt aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa
     4921 atatcataaa aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc
     4981 atcaccgact tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc
     5041 caaaaccttt ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa
     5101 tttcaacatc attgcagcag acaagatagt ggcgataggg ttgaccttat tctttggcaa
     5161 atctggagca gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa
     5221 agaggccaag gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc
     5281 ggagatgata tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt
     5341 cttaactagg atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtaggaaa
     5401 ttcgttcttg atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt
     5461 agctttatcc aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc
     5521 cattcttgtg attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc
     5581 atcaccatcg tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac
     5641 aacgaagtca gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc
     5701 ggatgcaaag ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag
     5761 taaaccttgt tcaggtctaa cactacctgt accccattta ggaccaccca cagcacctaa
     5821 caaaacggca tcagccttct tggaggcttc cagcgcctca tctggaagtg ggacacctgt
     5881 agcttcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg
     5941 aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg
     6001 gtcacctggc aaaacgacga tcttcttagg ggcagacatt agaatggtat atccttgaaa
     6061 tatatatata tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct
     6121 aaccacctat tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg
     6181 atgcaagcat ttagtcatga acgcttctct attctatatg aaaagccggt tccggcgctc
     6241 tcacctttcc tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc
     6301 tgaaattaac aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt
     6361 gttctcgtta tgttgaggaa aaaaataatg gttgctaaga gattcgaact cttgcatctt
     6421 acgatacctg agtattccca cagttggggg atctcgactc tagctagagg atcaattcgt
     6481 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6541 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgataac
     6661 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
     6721 gctagcttgg ctgcaggtcg agcggccgcg atccggaacc cttaatataa cttcgtataa
     6781 tgtatgctat acgaagttat cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8761 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8821 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     8881 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8941 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     9001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataacgca tttaagcata
     9061 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     9121 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     9181 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagcta gaaagtatag
     9241 gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa
     9301 aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg
     9361 ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc
     9421 acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct
     9481 agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac
     9541 aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt
     9601 tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg
     9661 cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc
     9721 gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag
     9781 aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat
     9841 caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta
     9901 gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt
     9961 tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
    10021 gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc
    10081 ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat
    10141 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag
    10201 atgcttcgtt gcttgcatgc c
//
