LOCUS       sequence-38784-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0050
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..196
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggtgccgcgc ggcagccata tggcctctgg agccgattca aaaggtgatg atttatcaac
      241 agccattctc aaacagaaga accgacccaa tcggttaatt gttgatgaag ccatcaatga
      301 agataacagc gtggtgtcct tgtcccagcc caagatggat gaactgcagt tgttccgagg
      361 tgacacggtg ttgctaaaag gaaagaaaag acgggaagct gtatgcattg ttctttctga
      421 tgacacgtgt tctgatgaga agattcgaat gaatagagtt gttcggaata acctccgagt
      481 tcgcctagga gatgtcatca gcatccagcc atgccctgat gtaaagtatg gcaaacgtat
      541 ccacgttcta cccatcgatg acacagtgga aggcatcact ggcaatctct ttgaggtata
      601 ccttaagccg tacttcctgg aagcttatcg gcccatccgt aaaggagata tttttcttgt
      661 ccggggtggg atgcgtgctg tggagttcaa agttgtagag acagatccca gcccttactg
      721 tattgttgct ccagacacag tgatccactg tgagggggag ccaatcaaac gagaggatga
      781 ggaggaatcc ttgaatgaag taggctatga tgacgtcgg
//
