LOCUS       sequence-387855-       11109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a EGFP-tagged full length human 53BP1 
            (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Tiwari A, Addis Jones O, Chan KL
  TITLE     53BP1 can limit sister-chromatid rupture and rearrangements driven 
            by a distinct ultrafine DNA bridging-breakage process.
  JOURNAL   Nat Commun. 2018 Feb 14;9(1):677. doi: 10.1038/s41467-018-03098-y.
  PUBMED    29445165
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-14"; volume: "9(1):677. doi"; pages: " 
            10.1038/s41467-018-03098-y"
COMMENT     Sequence Label: pEGFP-h53BP1 (siRNA resistant)
FEATURES             Location/Qualifiers
     source          1..11109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        157..460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        461..664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     615..635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      703..712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1015..1034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7575..7592)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7802..7821
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     polyA_signal    7993..8114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8030..8049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8084..8103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8121..8576)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8258..8279)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8470..8489
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8603..8707
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8709..9066
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8709..8900
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8732..8752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8917..9052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9101..9895
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9101..9895
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9155..9174)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9765..9784
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10083..10102)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10127..10174
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10503..11091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10992..11011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
      121 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      181 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      421 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      481 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      541 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      601 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      661 agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      901 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1141 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1201 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgag
     1441 atggacccta ctggaagtca gttggattca gatttctctc agcaagatac tccttgcctg
     1501 ataattgaag attctcagcc tgaaagccag gttctagagg atgattctgg ttctcacttc
     1561 agtatgctat ctcgacacct tcctaatctc cagacgcaca aagaaaatcc tgtgttggat
     1621 gttgtgtcca atcctgaaca aacagctgga gaagagcggg gcgacggtaa tagtgggttc
     1681 aatgaacatt tgaaagaaaa caaggttgca gaccctgtgg attcttctaa cttggacaca
     1741 tgtggttcca tcagtcaggt cattgagcag ttacctcagc caaacaggac aagcagtgtt
     1801 ctgggaatgt cagtggaatc tgctcctgct gtggaggaag agaagggaga agagttggaa
     1861 cagaaggaga aagagaagga agaagatact tcaggcaata ctacacattc ccttggtgct
     1921 gaagatactg cctcatcaca gttgggtttt ggggttctgg aactctccca gagccaggat
     1981 gttgaggaaa atactgtgcc atatgaagtg gacaaagagc agctacaatc agtaaccacc
     2041 aactctggtt ataccaggct gtctgatgtg gatgctaata ctgcaattaa gcatgaagaa
     2101 cagtccaacg aagatatccc catagcagaa cagtccagca aggacatccc tgtgacagca
     2161 cagcccagta aggatgtaca tgttgtaaaa gagcaaaatc caccacctgc aaggtcagag
     2221 gacatgcctt ttagccccaa agcatctgtt gctgctatgg aagcaaaaga acagttgtct
     2281 gcacaagaac ttatggaaag tggactgcag attcagaagt caccagagcc tgaggttttg
     2341 tcaactcagg aagacttgtt tgaccagagc aataaaacag tatcttctga tggttgctct
     2401 actccttcaa gggaggaagg tgggtgttct ttggcttcca ctcctgccac cactctgcat
     2461 ctcctgcagc tctctggtca gaggtccctt gttcaggaca gtctttccac gaattcttca
     2521 gatcttgttg ctccttctcc tgatgctttc cgatctactc cttttatcgt tcctagcagt
     2581 cccacagagc aagaagggag acaagataag ccaatggaca cgtcagtgtt atctgaagaa
     2641 ggaggagagc cttttcagaa gaaacttcaa agtggtgaac cagtggagtt agaaaacccc
     2701 cctctcctgc ctgagtccac tgtatcacca caagcctcaa caccaatatc tcagagcaca
     2761 ccagtcttcc ctcctgggtc acttcctatc ccatcccagc ctcagttttc tcatgacatt
     2821 tttattcctt ccccaagtct ggaagaacaa tcaaatgatg ggaagaaaga tggagatatg
     2881 catagttcat ctttgacagt tgagtgttct aaaacttcag agattgaacc aaagaattcc
     2941 cctgaggatc ttgggctatc tttgacaggg gattcttgca agttgatgct ttctacaagt
     3001 gaatatagtc agtccccaaa gatggagagc ttgagttctc acagaattga tgaagatgga
     3061 gaaaacacac agattgagga tacggaaccc atgtctccag ttctcaattc taaatttgtt
     3121 cctgctgaaa atgatagtat cctgatgaat ccagcacagg atggtgaagt acaactgagt
     3181 cagaatgatg acaaaacaaa gggagatgat acagacacca gggatgacat tagtatttta
     3241 gccactggtt gcaagggcag agaagaaacg gtagcagaag atgtttgtat tgatctcact
     3301 tgtgattcgg ggagtcaggc agttccgtca ccagctactc gatctgaggc actttctagt
     3361 gtgttagatc aggaggaagc tatggaaatt aaagaacacc atccagagga ggggtcttca
     3421 gggtctgagg tggaagaaat ccctgagaca ccttgtgaaa gtcaaggaga ggaactcaaa
     3481 gaagaaaata tggagagtgt tccgttgcac ctttctctga ctgaaactca gtcccaaggg
     3541 ttgtgtcttc aaaaggaaat gccaaaaaaa gaatgctcag aagctatgga agttgaaacc
     3601 agtgtgatta gtattgattc ccctcaaaag ttggcaatac ttgaccaaga attggaacat
     3661 aaggaacagg aagcttggga agaagctact tcagaggact ccagtgttgt cattgtagat
     3721 gtgaaagagc catctcccag agttgatgtt tcttgtgaac ctttggaggg agtggagaag
     3781 tgctcagatt cccagtcatg ggaggatatt gctccagaaa tagaaccatg tgctgagaat
     3841 agattagaca ccaaggaaga aaagagtgta gaatatgaag gagatctgaa atcagggact
     3901 gcagaaacag aacctgtaga gcaagattct tcacagcctt ccttaccttt agtgagagca
     3961 gatgatcctt taagacttga ccaggagttg cagcagcccc aaactcagga gaaaacaagt
     4021 aattcattaa cagaagactc aaaaatggct aatgcaaagc agctaagctc agatgcagag
     4081 gcccagaagc tggggaagcc ctctgcccat gcctcacaaa gcttctgtga aagttctagt
     4141 gaaaccccat ttcatttcac tttgcctaaa gaaggtgata tcatcccacc attgactggt
     4201 gcaaccccac ctcttattgg gcacctaaaa ttggagccca agagacacag tactcctatt
     4261 ggtattagca actatccaga aagcaccata gcaaccagtg atgtcatgtc tgaaagcatg
     4321 gtggagaccc atgatcccat acttgggagt ggaaaagggg attctggggc tgccccagac
     4381 gtggatgata aattatgtct aagaatgaaa ctggttagtc ctgagactga ggcgagtgaa
     4441 gagtctttgc agttcaacct ggaaaagcct gcaactggtg aaagaaaaaa tggatctact
     4501 gctgttgctg agtctgttgc cagtccccag aagaccatgt ctgtgttgag ctgtatctgt
     4561 gaagccaggc aagagaatga ggctcgaagt gaggatcccc ccaccacacc catcaggggg
     4621 aacttgctcc actttccaag ttctcaagga gaagaggaga aagaaaaatt ggagggtgac
     4681 catacaatca ggcagagtca acagcctatg aagcccatta gtcctgtcaa ggaccctgtt
     4741 tctcctgctt cccagaagat ggtcatacaa gggccatcca gtcctcaagg agaggcaatg
     4801 gtgacagatg tgctagaaga ccagaaagaa ggacggagta ctaataagga aaatcctagt
     4861 aaggccttga ttgaaaggcc cagccaaaat aacataggaa tccaaaccat ggagtgttcc
     4921 ttgagggtcc cagaaactgt ttcagcagca acccagacta taaagaatgt gtgtgagcag
     4981 gggaccagta cagtggacca gaactttgga aagcaagatg ccacagttca gactgagagg
     5041 gggagtggtg agaaaccagt cagtgctcct ggggatgata cagagtcgct ccatagccag
     5101 ggagaagaag agtttgatat gcctcagcct ccacatggcc atgtcttaca tcgtcacatg
     5161 agaacaatcc gggaagtacg cacacttgtc actcgtgtca ttacagatgt gtattatgtg
     5221 gatggaacag aagtagaaag aaaagtaact gaggagactg aagagccaat tgtagagtgt
     5281 caggagtgtg aaactgaagt ttccccttca cagactgggg gctcctcagg tgacctgggg
     5341 gatatcagct ccttctcctc caaggcatcc agcttacacc gcacatcaag tgggacaagt
     5401 ctctcagcta tgcacagcag tggaagctca gggaaaggag ccggaccact cagagggaaa
     5461 accagcggga cagaacccgc agattttgcc ttacccagct cccgaggagg cccaggaaaa
     5521 ctgagtccta gaaaaggggt cagtcagaca gggacgccag tgtgtgagga ggatggtgat
     5581 gcaggccttg gcatcagaca gggagggaag gctccagtca cgcctcgtgg gcgtgggcga
     5641 aggggccgcc caccttctcg gaccactgga accagagaaa cagctgtgcc tggccccttg
     5701 ggcatagagg acatttcacc taacttgtca ccagatgata aatccttcag ccgtgtcgtg
     5761 ccccgagtgc cagactccac cagacgaaca gatgtgggtg ctggtgcttt gcgtcgtagt
     5821 gactctccag aaattccttt ccaggctgct gctggccctt ctgatggctt agatgcctcc
     5881 tctccaggaa atagctttgt agggctccgt gttgtagcca agtggtcatc caatggctac
     5941 ttttactctg ggaaaatcac acgagatgtc ggagctggga agtataaatt gctctttgat
     6001 gatgggtacg aatgtgatgt gttgggcaaa gacattctgt tatgtgaccc catcccgctg
     6061 gacactgaag tgacggccct ctcggaggat gagtatttca gtgcaggagt ggtgaaagga
     6121 cataggaagg agtctgggga actgtactac agcattgaaa aagaaggcca aagaaagtgg
     6181 tataagcgaa tggctgtcat cctgtccttg gagcaaggaa acagactgag agagcagtat
     6241 gggcttggcc cctatgaagc agtaacacct cttacaaagg cagcagatat cagcttagac
     6301 aatttggtgg aagggaagcg gaaacggcgc agtaacgtca gctccccagc cacccctact
     6361 gcctccagta gcagcagcac aacccctacc cgaaagatca cagaaagtcc tcgtgcctcc
     6421 atgggagttc tctcaggcaa aagaaaactt atcacttctg aagaggaacg gtcccctgcc
     6481 aagcgaggtc gcaagtctgc cacagtaaaa cctggtgcag taggggcagg agagtttgtg
     6541 agcccctgtg agagtggaga caacaccggt gaaccctctg ccctggaaga gcagagaggg
     6601 cctttgcctc tcaacaagac cttgtttctg ggctacgcat ttctccttac catggccaca
     6661 accagtgaca agttggccag ccgctccaaa ctgccagatg gtcctacagg aagcagtgaa
     6721 gaagaggagg aatttttgga aattcctcct ttcaacaagc agtatacaga atcccagctt
     6781 cgagcaggag ctggctatat ccttgaagat ttcaatgaag cccagtgtaa cacagcttac
     6841 cagtgtcttc taattgcgga tcagcattgt cgaacccgga agtacttcct gtgccttgcc
     6901 agtgggattc cttgtgtgtc tcatgtctgg gtccatgata gttgccatgc caaccagctc
     6961 cagaactacc gtaattatct gttgccagct gggtacagcc ttgaggagca aagaattctg
     7021 gactggcaac cccgtgaaaa tcctttccag aatctgaagg tactcttggt atcagaccaa
     7081 cagcagaact tcctggagct ctggtctgag atcctcatga ctggtggtgc agcctctgtg
     7141 aagcagcacc attcaagtgc ccataacaaa gatattgctt taggggtatt tgatgtggtg
     7201 gtgacggacc cctcatgccc agcctcggtg ctgaagtgtg ctgaagcatt gcagctgcct
     7261 gtggtgtcac aagagtgggt gatccagtgc ctcattgttg gggagagaat tggattcaag
     7321 cagcatccaa aatataaaca cgattatgtt tctcactaag cggccgcatc gagagatccg
     7381 ggagacacaa cggtttccct ctagcgggat caattccgcc ccccccccta acgttactgg
     7441 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7501 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7561 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7621 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7681 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7741 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7801 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccgcgggc
     7861 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     7921 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7981 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8101 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8161 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8221 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8281 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8341 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8401 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8461 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8521 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     8581 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8641 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8701 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     8761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8821 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9001 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9061 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     9121 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     9181 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     9241 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     9301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     9361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     9421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     9481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     9541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     9601 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     9661 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     9721 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     9781 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     9841 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     9901 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     9961 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10021 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
    10081 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    10141 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    10201 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    10261 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    10321 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
    10381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11101 aacgcggcc
//
