LOCUS       sequence-387857-       15013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene 
            promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by 
            ribozymes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: 
            pC1300_pUB10_pco-nCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
FEATURES             Location/Qualifiers
     source          1..15013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..26)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(23..45)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     928..948
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4195..4214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4448..4465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4480..4620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4546..4565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4706..4728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4964..5158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5224..6297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6726..7355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8951..9125
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     ncRNA           complement(9140..9207)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(9264..9300)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     intron          12749..12937
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             14256..14276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14283..14303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14322..14369
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2821 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2881 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2941 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3001 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3061 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3121 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3181 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3241 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3301 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3361 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3421 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3481 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3541 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3601 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4501 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4621 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4681 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4741 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4801 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4861 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4921 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4981 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     5041 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     5101 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     5161 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     5221 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     5281 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     5341 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     5401 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5461 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5521 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5581 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5641 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5701 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5761 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5821 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5881 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5941 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     6001 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     6061 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     6121 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     6181 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     6241 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta gatcactagt
     8941 taattcgggg gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     9001 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     9061 agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg
     9121 agaaactcga gttaattaag tcccattcgc catgccgaag catgttgccc agccggcgcc
     9181 agcgaggagg ctgggaccat gccggccatt ggctggattg tcaactggtc ccctcgtgag
     9241 gggcaatcgt tgagcgttcc gacgacgagc ttactcgttt cgtcctcacg gactcatcag
     9301 gtcggagctg aggcaagctt agctcttacc tgttttcgtc gtcttggctt ttcttcttcc
     9361 tggccacgtg cctgaatact tcgccgcctt gactacttct aggctacttg ctctctttct
     9421 ctctctagga ctatctctct gagattttgc tcccttaaca atgagggagg ggctaagtat
     9481 ttatagactg acgggtgagt ggacatttcc caaactaccc ttacttattt cgtaagccct
     9541 tacgtcattg ctccattatt ggagtctgaa gatgccttca catggtggac ccccacctgt
     9601 cggccacgaa tcttatttgt tcgtcagaaa aagccaaacc gactgcacag tttttccatc
     9661 gtggtaggga ccactttggt attgattaaa ggcagccgac ctaacctttg tcagacgcat
     9721 tatttcacgc gttttctttt acagattcca tcttcatttg tgggacagta tgtctgccac
     9781 tagtatccta ggaaggtacc agtctagctc aacagagctt ttaacccaaa ttggtacaat
     9841 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
     9901 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
     9961 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
    10021 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
    10081 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
    10141 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
    10201 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
    10261 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
    10321 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
    10381 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
    10441 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
    10501 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
    10561 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
    10621 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
    10681 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
    10741 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
    10801 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
    10861 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
    10921 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
    10981 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
    11041 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
    11101 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
    11161 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
    11221 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
    11281 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
    11341 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
    11401 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
    11461 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
    11521 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
    11581 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
    11641 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
    11701 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
    11761 ttaatctgag tttttctgat taacagatgc cgaagcccgc cgtcgaatca gagttttcca
    11821 aagtcctcaa gaaacacttt cctggggagc gttttaggtc tagctatatg aagagggggg
    11881 gtaaaattct ggcagcacaa ggcgaggaag ctgtagtggc gtacttgcag ggaaagagtg
    11941 aggaggaacc gccgaatttt cagccgccgg cgaagtgcca cgtggtcacc aaaagcaggg
    12001 atttcgcaga atggcccata atgaaagcct ctgaagccat acagaggtac atctatgcgc
    12061 tcagcactac agagcgagct gcctgcaaac cgggtaagag ctcagaaagt cacgcggcct
    12121 ggttcgcggc tacaggggtg agcaatcacg gctattctca tgtacaaggt cttaacctga
    12181 tctttgacca cacgctagga cgatacgatg gcgttttaaa gaaagtacag cttcgaaatg
    12241 agaaggcccg agctagattg gctgcaataa acgccgctcg agctgcagct ggattgccgg
    12301 aaatcaaggc cgaggaggag gaggtagcta caaatgaaac aggtcatcta ctacagccgc
    12361 caggcataaa cccatcattc tacgtctacc agaccatatc tccgcaggct taccgaccaa
    12421 gggacgaaat agtgttacca cccgagtacg ccggttacgt cagggatccg aacgctccga
    12481 ttccactggg cgtggtcagg aaccgttgtg acatacagaa gggttgcccg ggatatatac
    12541 ccgagtggca gagggaagct ggtacggcaa ttagtcccaa gacaggaaaa gcagtgacgg
    12601 ttccaggact tagcccgaag aagaataaac gtatgcgtag gtactggagg tcagaaaagg
    12661 agaaggctca agatgcactt ctcgtaactg taaggatagg taccgattgg gtagtcatag
    12721 acgtaagagg attactaaga aacgccaggt aagtttctgc ttctaccttt gatatatata
    12781 taataattat cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag
    12841 aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa
    12901 cttttctaat atatgaccaa aatttgttga tgtgcaggtg gaggactatt gctcccaagg
    12961 acataagttt aaatgcactt ctagatttat ttaccggtga tccagtcatc gatgtcagac
    13021 gaaacatcgt gaccttcacc tataccttgg acgcttgcgg aacttatgct agaaaatgga
    13081 ctctcaaggg aaaacagaca aaagcaacct tagataaact gacagcgaca caaactgtgg
    13141 ccttagttgc tatagatctg ggacaaacaa acccaattag cgcgggtatc agtcgtgtca
    13201 cacaggagaa cggggccctc cagtgcgaac cgcttgatcg ttttacattg cctgatgacc
    13261 ttttgaaaga tatttctgcg taccgaattg catgggaccg taacgaggag gaactcaggg
    13321 ccagatccgt tgaggcactc ccagaggcac aacaagcaga ggtcagagct ctggacgggg
    13381 tctccaaaga gaccgcgcgt acacagttgt gcgcggactt cggtctggac ccaaagcgac
    13441 taccgtggga taaaatgagt agcaatacca cgttcataag cgaggcgctc ctttccaaca
    13501 gcgtatcccg tgaccaagta ttctttaccc cggccccaaa gaaaggagcc aagaagaaag
    13561 caccggtgga agtgatgcga aaagacagga catgggcgcg agcgtacaaa ccacgactct
    13621 cagtagaagc acaaaagttg aaaaatgagg ctctttgggc tttgaagcgt acctctccag
    13681 aatatctaaa gttgtcacga cgtaaagaag aattgtgtag gaggtccatt aattacgtga
    13741 tagaaaaaac tagaaggcga acccaatgcc aaattgtgat ccccgttata gaagatctaa
    13801 atgtccgatt tttccacggg tctggcaaac gactcccggg ctgggataac ttcttcacgg
    13861 caaagaagga aaaccgatgg tttatccaag ggctacataa ggcgttttct gacttaagga
    13921 cccaccgatc cttctacgtg ttcgaggtgc gtccagagag aacatcaatc acctgtccga
    13981 aatgcgggca ctgtgaagtg gggaacaggg atggagaagc ctttcagtgt ctcagttgcg
    14041 gtaagacatg caacgcagat ctggacgtag cgacacataa cttgactcaa gtggcgctca
    14101 ccggcaaaac aatgccgaag agagaggagc ctagagatgc acaagggaca gcccccgcga
    14161 gaaagacgaa gaaagctagc aagtcaaagg cacccccggc tgaacgtgag gatcagactc
    14221 ccgctcaaga gccgtcccaa acgtccggat ccggaccgaa gaaaaagcga aaggtagagg
    14281 atcctaaaaa gaagcgtaaa gtctccttgg gttctggctc cgactataag gatgacgatg
    14341 acaaagacta taaggatgac gacgacaagt gaagagcttt cgttcgtatc atcggtttcg
    14401 acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag
    14461 tttgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt
    14521 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga
    14581 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct
    14641 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa
    14701 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta
    14761 gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc
    14821 aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta
    14881 tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat agttatactc
    14941 atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt gccaattgat
    15001 tgacaacgaa ttc
//
