LOCUS       sequence-387865-        1972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F24
FEATURES             Location/Qualifiers
     source          1..1972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(217..805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(297..316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(976..1767,1768..1836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1599..1618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1837..1941)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcgtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
       61 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
      121 ttttcggcga ggacccatcg agaccatcgg aaagaacatg tgagcaaaag gccagcaaaa
      181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1081 cttaccatct ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga
     1141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
