LOCUS       sequence-387867-        2075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F3
FEATURES             Location/Qualifiers
     source          1..2075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(320..908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(400..419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1079..1870,1871..1939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1702..1721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1940..2044)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcgggcag taaggcggtc gggatagttt tcttgcggcc ctaatccgag
       61 ccagtttacc cgctctgcta cctgcgccag ctggcagttc aggccaatcc gcgccggatg
      121 cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc atttgaccac taccatcaat
      181 ccggtaggtt ttccggctga taaataaggt tttcccctga tgctgccacg cgtgagcggt
      241 ccgagaccat cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1201 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     1261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2041 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
