LOCUS       sequence-387868-        2061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F12
FEATURES             Location/Qualifiers
     source          1..2061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(318..906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(398..417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1077..1868,1869..1937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1700..1719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1938..2042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctgacg tcccaatgat ggtctcgata gagattcggg atttcggcgc tccacagttt
       61 cgggttttcg acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat
      121 aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga
      181 aactgttacc cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac
      241 gagaccatcg gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1201 gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag
     1261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2041 cgcacatttc cccgaaaagt g
//
