LOCUS       sequence-387869-        5789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Kv1.5 potassium 
            voltage-gated channel subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5789)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Kv1.5/RBG4
FEATURES             Location/Qualifiers
     source          1..5789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     123..142
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        123..141
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        160..463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        464..667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     618..638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     664..688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           757..796
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             826..852
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          1647..2219
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2351..2370)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2416..2471
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2470..2489)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      2825..2960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3066..3083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3237..3825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3317..3336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3996..4787,4788..4856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4619..4638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4857..4961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5029..5047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5085..5107)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5207..5226
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5293..5748)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5430..5451)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5642..5661
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tgtaatacga ctcactatag ggcgaattcg agctcgcccc gttacataac ttacggtaaa
      181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      421 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      481 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      541 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      601 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      661 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      721 ccatagaaga caccgggacc gatccagcct ccgggggatc ttggtggcgt gaaactcccg
      781 cacctcttcg gccagcgcct tgtagaagcg cgtatggctt ctagctatcc ttatgacgtg
      841 cctgactatg ccagcctggg aggaccttct agaattcgag ctcggtaccc ggggatctcc
      901 ctggtgcccc tggagaatgg cagtgccatg accctcagag gaggagggga ggcaggggca
      961 agctgtgtgc agacccccag gggagagtgt gggtgccctc cgacgtctgg actcaataat
     1021 cagtccaaag aaacactgtt gagggggcgc acaacgctcg aggatgcgaa ccagggtgga
     1081 cggcctttgc cccctatggc tcaggagctg ccacaaccta gaaggctatc tgctgaggat
     1141 gaggagggag aaggcgaccc tggcctgggc acagtggagg aggaccaggc tcctcaggat
     1201 gcagggtcac tccatcacca gcgagtcctc ataaacatct ccgggttgcg tttcgagacg
     1261 cagctgggca ccctggcaca gtttcccaac accctcctgg gggacccagc caagcgcctg
     1321 cactacttcg accccttgag aaatgaatac ttcttcgacc gcaaccggcc cagcttcgat
     1381 ggcattttgt actactacca gtctgggggc cgcctgcgca ggcccgtcaa tgtctccctg
     1441 gatgtgtttg cagatgagat ccgcttttac cagctggggg acgaggccat ggagcgcttc
     1501 cgggaggatg agggcttcat caaggaagag gagaagcccc tgccccgcaa cgagttccag
     1561 cgccaggtat ggcttatctt cgaataccca gaaagctctg ggtccgcaag agccatcgcc
     1621 atagtgtcgg tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
     1681 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
     1741 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
     1801 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
     1861 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
     1921 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
     1981 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
     2041 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca
     2101 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
     2161 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc
     2221 tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc
     2281 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     2341 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     2401 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     2461 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     2521 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     2581 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     2641 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     2701 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     2761 tcccagtcat agctgtccct cttctcttat ggagatccct cgaggagctt tttgcaaaag
     2821 cctaggcctc caaaaaagcc tcttcactac ttctggaata gctcagaggc cgaggcggcc
     2881 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     2941 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     3001 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5041 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5221 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5281 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5341 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5401 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5461 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5521 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5581 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5641 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5701 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     5761 caggctgcgc aactgttggg aagggcgat
//
