LOCUS       sequence-387880-        8265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the GluR2/GluA2 AMPA 
            receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GluR2 FL/pRK5
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..245
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     202..222
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    314..448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(338..357)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     392..411
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      452..461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4074..4090)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4074..4090)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4148..4167)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(4180..4198)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4181..4198)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4408..4432)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4429..4632)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4458..4478)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4633..5012
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(5041..5057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5041..5057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5054..5076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5065..5081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5089..5095,5096..5113,5114..5119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5134..5155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5272..5289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5443..6031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5523..5542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6202..6993,6994..7062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6825..6844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7063..7167)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7235..7253
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7291..7313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7413..7432
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7449..7904)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7586..7607)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7798..7817
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8102..8124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8116..8133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8117..8133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(join(8153..8265,1..245))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8167..8265,1..37)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
       61 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
      121 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
      181 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
      241 ctcagaaggt acctaaccaa gttcctcttt cagaggttat ttcaggccat ggtgctgcgc
      301 cgaattaatt cccgatccag acatgataag atacattgat gagtttggac aaaccacaac
      361 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      421 aaccattata agctgcaata aacaagttgg gccatggcgg ccaagcttct gcaggtcgag
      481 tttttttttt ttttttttgg tttttgtttt gttttgtttt tgtttttgta tttgtcttaa
      541 ccaaccagta cggaagcaaa tttatttaaa agccagtttg tctgtgaaaa tacctaaaaa
      601 tattttttta ttttaaatgt ttttttcctt tttatttata aaacaggtca catcttgatg
      661 cagttgatgt caagtgtgct taagtcatta tgaatcaaga gactaacaat agtggctgcg
      721 gaaacgggtt tgttgtctgt acaaagactt cagttaaatg atagtatttc catgttagct
      781 gtgcatgtcc accacgcttc ctctgatatc ggagtagaaa atgatgctct gtttgtaatt
      841 tatctttcct tacaattcaa ggtgaactcc acatagttaa gaattcttgg ctgaaagaaa
      901 agccttcaag atatcggatg cctctcacca ctttgacaat aaacacacaa ggaaaccatt
      961 gtgtaaggca ctcagaaggt tcctatcaat cacgagagat cagtcacact gacattcatt
     1021 cccatgccag gactcacgta agggacagca tgcactgctt tgggaaattc tggagtcata
     1081 acacgtccat tttctccagt acttcctgta attgacagcc ttgccttgct cctcatgaca
     1141 tcactcaagg tcatccccta aattttaaca ctctcgatgc catatacgtt gtaaccttcc
     1201 ttataagttg caaaattctg ggaattctgc gaggaagatg ggttaatatt ctgtggattc
     1261 tttgccacct tcattcgttt cgcctcggcc cttgacttgt aacagaactc aatcaaagcc
     1321 accagcattg ccaaaccaag gcccccgaca aggatgtaga atactccagc aacgttgctc
     1381 agactgaggg cactggtctt ttccttactt cccgagtcct tggctccaca ttcaccttta
     1441 tcgtaccacc atttgttttt cagcttgtct aagacgcctt gctcactgag tttcaatact
     1501 gcaagattta ctggggttcc taatgaggat cctttaggtg tggcgatgcc gtagcctttg
     1561 gaatccaagt ttcctcccac tttcatggtg tcacaaggct tcctctgctc gatgtactcg
     1621 ttcattgtgg actccagcaa gtaggcatac tttcctttgg atttccggac tctggctact
     1681 ccttctgcgg tagtcctcac aaacacagag ggctctgcac tcctcatata agtccacatt
     1741 ttatcaaaca ctgcgatttt agatctcctg aaaaactctt tagtggagcc agagtctaat
     1801 gttccataag caatttctgt ttgcttagac agatcctcag cactttcgat gggagacacc
     1861 atcctctcta cagtcaggaa ggcagctaag ttagccgtgt aggaggagat tatgatcagg
     1921 gtaaagaacc accacacacc tccaacaatg cgcccagaga gggatcttgg cgaaatatcg
     1981 catccttgcc gcataaaggc acccaaggaa aaccagagac tattaaaaat cccaaattca
     2041 ttagttgatt cactactttg tgtttctctt ccatcttcaa attcctcagt gtgccactcg
     2101 taggggctaa atctgctgac caggaataaa actacactga ccccaatgta ggcaaacaca
     2161 atgcacatcc agatctcata ggctaaagga tcaagaaagg aaaacactcc tggtttggac
     2221 ttctgaggct tcttgatcat gatagagatt ccaagactca tgaagggctt ggagaagtca
     2281 atcacctctt ctctcacgag agtgatagtt aatggagcaa ttgcaatgtc agctttcccg
     2341 tagacaagct ctccaaccat accattccaa attttggtgt cggcatccct ggccccatac
     2401 ttgccatccc caacaatagt cagcttgtac ttgaacccac agtgtttggc aatttctgca
     2461 gctaagtcaa cacagtagcc ctcgtaacgc tcattccctt caagcatttc atgatttttc
     2521 ttcatcataa catatggaga ttccaatatt gtggtgacca ccacagtctt gttttcaagc
     2581 ccagacgtgt catttcctga tgggagctca gttagggtga caaccatttt atccacttca
     2641 ctccagtacc caatcttccg gggtccattt gttttgagct ccatgatgtt aattgtgtag
     2701 tttattcgtt ttccattctg gtcaaacttt atatttccag agaggccttc aacttgaacc
     2761 tgcttgaggg ccctttctat ttcgacccct tgtccccagg gcacagctgg gttggccaaa
     2821 caatcccctg catttcctct ccgggatatt tcaatcctct gcttccgaag gttacggaat
     2881 gcttcagtca tcacttggac agcatcatac gtcagggccg aagtatactt aattgtcgct
     2941 gtgtgtgctc cagggtattc tttctcttcc agtgttgacc atctttctat aaatttagac
     3001 accagggaat catcgtagtc tacaatctga aatccagaga catttgctcc tccaaactga
     3061 attttcagca ggtccccatc agtgaatccc agatttgcaa tgatataatg gtacccttta
     3121 acatgttttc caatggtaat aacctggtcc acaatgtcat ttactttatc cctttcacag
     3181 tccaggatta cacgccgttc tttttttaac tccagatctt gaaagagcga tctgtaggtc
     3241 tcatctttct tgtcattgtt gatgttcccc acattgatag cagtcacctg ccacttcttc
     3301 tctgcagcag aatccagaac agcttgcagt gttgataagc ctctgtcact gtcatagagg
     3361 tatgcgaact tgtcccattg gtagtactca atcaagctaa ggagtgctcc tttgaggtca
     3421 ggtcgcatct ggatgacaaa tggatgtgtg ccatctgttg ggaagctagg tgtgatgaag
     3481 gacacatgga gtgtcccaca gaatgatgtg atggtattta cagacttctt gtcataaaat
     3541 ccaaaaattg cgtagactcc tcttgaaaac tgggagcaga aagcattggt gactgcgaaa
     3601 ctgttggcta cctccaaatt gtcgatatgg ggtgtcagtc tgaactccga agtggaaaac
     3661 tgaaccatcc ctacccgaaa tgcactgtat tcttgatcag cgccccttgg aaatagcccc
     3721 cctatctgta tgctgttaga gcgcgcagag acaccaaaaa tcagtcccca taaaacagga
     3781 gaaaggagga cagaaatatg cataatcttt tgcatttcca agaaaagtag agcatccaca
     3841 aaatatcccc tcgtttccct ttcttcctct tccttctttg actgttttcc tcagcaaatt
     3901 agatcctctg cattgtgagg tcgatattta gcaaggagct gctaaaaaca cgaagtgaag
     3961 gcagaagaat ccttatttcc cagttgtagc tggtggctgt tgatgccttg cgtcctgtcc
     4021 ctgctcccga agtcccgagg actggcctcg tgccgaattc ctgcagcccg ggggatccac
     4081 tagttctaga ggatccccgg ggaattcaat cgatagaacc gaggtgcagt tggacctggg
     4141 agtggacacc tgtggagaga aaggcaaagt ggatgttatt ctatagtgtc acctaaatcg
     4201 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg aagccaaggg
     4261 ggtgggccta tagactctat aggcggtact tacgtcactc ttggcacggg gaatccgcgt
     4321 tccaatgcac cgttcccggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc
     4381 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
     4441 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     4501 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     4561 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     4621 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     4681 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     4741 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     4801 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     4861 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     4921 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     4981 ttactattaa taactagtca ataatcaatg tcgggcgagc tcgaattaat tcgtaatcat
     5041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7201 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7261 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     7321 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     7381 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     7441 gcaccataaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     7501 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     7561 gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     7621 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     7681 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     7741 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     7801 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     7861 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtactatg gttgctttga
     7921 cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     7981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8041 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8101 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgttaacagc tttttgcaaa
     8161 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     8221 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcg
//
