LOCUS       sequence-387881-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6155..6667
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6743..6761)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6798..6919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6809..6828)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6863..6882
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7164..7521
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7164..7355
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7187..7207)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7372..7507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7552..8589
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8568..8576
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8613..8661
                     /note="synthetic polyadenylation signal"
     promoter        8817..8921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8922..8990,8991..9782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9140..9159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9940..10528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10020..10039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10644..10927
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgcca tgaaactgcg
     1081 tggagtcagc ctggctgccg gcttgttctt actggccctg agtctttggg ggcagcctgc
     1141 agaggctgcg gcttgctatg ggtgttctcc aggatcaaag tgtgactgca gtggcataaa
     1201 aggggaaaag ggagagagag ggtttccagg tttggaagga cacccaggat tgcctggatt
     1261 tccaggtcca gaagggcctc cggggcctcg gggacaaaag ggtgatgatg gaattccagg
     1321 gccaccagga ccaaaaggaa tcagaggtcc tcctggactt cctggatttc cagggacacc
     1381 aggtcttcct ggaatgccag gccacgatgg ggccccagga cctcaaggta ttcccggatg
     1441 caatggaacc aagggagaac gtggatttcc aggcagtccc ggttttcctg gtttacaggg
     1501 tcctccagga ccccctggga tcccaggtat gaagggtgaa ccaggtagta taattatgtc
     1561 atcactgcca ggaccaaagg gtaatccagg atatccaggt cctcctggaa tacaaggcct
     1621 acctggtccc actggtatac cagggccaat tggtccccca ggaccaccag gtttgatggg
     1681 ccctcctggt ccaccaggac ttccaggacc taaggggaat atgggcttaa atttccaggg
     1741 acccaaaggt gaaaaaggtg agcaaggtct tcagggccca cctgggccac ctgggcagat
     1801 cagtgaacag aaaagaccaa ttgatgtaga gtttcagaaa ggagatcagg gacttcctgg
     1861 tgaccgaggg cctcctggac ctccagggat acgtggtcct ccaggtcccc caggtggtga
     1921 gaaaggtgag aagggtgagc aaggagagcc aggcaaaaga ggtaaaccag gcaaagatgg
     1981 agaaaatggc caaccaggaa ttcctggttt gcctggtgat cctggttacc ctggtgaacc
     2041 cggaagggat ggtgaaaagg gccaaaaagg tgacactggc ccacctggac ctcctggact
     2101 tgtaattcct agacctggga ctggtataac tataggagaa aaaggaaaca ttgggttgcc
     2161 tgggttgcct ggagaaaaag gagagcgagg atttcctgga atacagggtc cacctggcct
     2221 tcctggacct ccaggggctg cagttatggg tcctcctggc cctcctggat ttcctggaga
     2281 aaggggtcag aaaggtgatg aaggaccacc tggaatttcc attcctggac ctcctggact
     2341 tgacggacag cctggggctc ctgggcttcc agggcctcct ggccctgctg gccctcacat
     2401 tcctcctagt gatgagatat gtgaaccagg ccctccaggc cccccaggat ctccaggtga
     2461 taaaggactc caaggagaac aaggagtgaa aggtgacaaa ggtgacactt gcttcaactg
     2521 cattggaact ggtatttcag ggcctccagg tcaacctggt ttgccaggtc tcccaggtcc
     2581 tccaggatct cttggtttcc ctggacagaa aggggaaaaa ggacaagctg gtgcaactgg
     2641 tcccaaagga ttaccaggca ttccaggagc tccaggtgct ccaggctttc ctggatctaa
     2701 aggtgaacct ggtgatatcc tcacttttcc aggaatgaag ggtgacaaag gagagttggg
     2761 ttcccctgga gctccagggc ttcctggttt acctggcact cctggacagg atggattgcc
     2821 agggcttcct ggcccgaaag gagagcctgg tggaattact tttaagggtg aaagaggtcc
     2881 ccctgggaac ccaggtttac caggcctccc agggaatata gggcctatgg gtccccctgg
     2941 tttcggccct ccaggcccag taggtgaaaa aggcatacaa ggtgtggcag gaaatccagg
     3001 ccagccagga ataccaggtc ctaaagggga tccaggtcag actataaccc agccggggaa
     3061 gcctggcttg cctggtaacc caggcagaga tggtgatgta ggtcttccag gtgaccctgg
     3121 acttccaggg caaccaggct tgccagggat acctggtagc aaaggagaac caggtatccc
     3181 tggaattggg cttcctggac cacctggtcc caaaggcttt cctggaattc caggacctcc
     3241 aggagcacct gggacacctg gaagaattgg tctagaaggc cctcctgggc cacccggctt
     3301 tccaggacca aagggtgaac caggatttgc attacctggg ccacctgggc caccaggact
     3361 tccaggtttc aaaggagcac ttggtccaaa aggtgatcgt ggtttcccag gacctccggg
     3421 tcctccagga cgcactggct tagatgggct ccctggacca aaaggtgatg ttggaccaaa
     3481 tggacaacct ggaccaatgg gacctcctgg gctgccagga ataggtgttc agggaccacc
     3541 aggaccacca gggattcctg ggccaatagg tcaacctggt ttacatggaa taccaggaga
     3601 gaagggggat ccaggacctc ctggacttga tgttccagga cccccaggtg aaagaggcag
     3661 tccagggatc cccggagcac ctggtcctat aggacctcca ggatcaccag ggcttccagg
     3721 aaaagcaggt gcctctggat ttccaggtac caaaggtgaa atgggtatga tgggacctcc
     3781 aggcccacca ggacctttgg gaattcctgg caggagtggt gtacctggtc ttaaaggtga
     3841 tgatggcttg cagggtcagc caggacttcc tggccctaca ggagaaaaag gtagtaaagg
     3901 agagcctggc cttccaggcc ctcctggacc aatggatcca aatcttctgg gctcaaaagg
     3961 agagaagggg gaacctggct taccaggtat acctggagtt tcagggccaa aaggttatca
     4021 gggtttgcct ggagacccag ggcaacctgg actgagtgga caacctggat taccaggacc
     4081 accaggtccc aaaggtaacc ctggtctccc tggacagcca ggtcttatag gacctcctgg
     4141 acttaaagga accatcggtg atatgggttt tccagggcct cagggtgtgg aagggcctcc
     4201 tggaccttct ggagttcctg gacaacctgg ctccccagga ttacctggac agaaaggcga
     4261 caaaggtgat cctggtattt caagcattgg tcttccaggt cttcctggtc caaagggtga
     4321 gcctggtctg cctggatacc cagggaaccc tggtatcaaa ggttctgtgg gagatcctgg
     4381 tttgcccgga ttaccaggaa cccctggagc aaaaggacaa ccaggccttc ctggattccc
     4441 aggaacccca ggccctcctg gaccaaaagg tattagtggc cctcctggga accccggcct
     4501 tccaggagaa cctggtcctg taggtggtgg aggtcatcct gggcaaccag ggcctccagg
     4561 cgaaaaaggc aaacccggtc aagatggtat tcctggacca gctggacaga agggtgaacc
     4621 aggtcaacca ggctttggaa acccaggacc gcctggactt ccaggacttt ctggccaaaa
     4681 gggtgatgga ggattacctg ggattccagg aaatcctggc cttccaggtc caaagggcga
     4741 accaggcttt cacggtttcc ctggtgtgca gggtccccca ggccctcctg gttctccggg
     4801 tccagctctg gaaggaccta aaggcaaccc tgggccccaa ggtcctcctg ggagaccagg
     4861 tcctacaggt tttcaaggtc taccaggtcc agaaggtcct ccaggtctcc ctggaaatgg
     4921 aggtattaaa ggagagaagg gaaatccagg ccaacctggg ctacctggct tgcctggttt
     4981 gaaaggagat caaggaccac caggactcca gggtaatcct ggccggccgg gtctcaatgg
     5041 aatgaaagga gatcctggtc tccctggtgt tccaggattc ccaggcatga aaggacccag
     5101 tggagtacct ggatcagctg gccctgaggg ggaaccggga cttattggtc ctccaggtcc
     5161 tcctggatta cctggtcctt caggacagag tatcataatt aaaggagatg ctggtcctcc
     5221 aggaatccct ggccagcctg ggctaaaggg tctaccagga ccccaaggac ctcaaggctt
     5281 accaggtcca actggccctc caggagatcc tggacgcaat ggactccctg gctttgatgg
     5341 tgcaggaggg cgcaaaggag acccaggtct gccaggacag ccaggtaccc gtggtttgga
     5401 tggtccccct ggtccagatg gattgcaagg tcccccaggt ccccctggaa cctcctctgt
     5461 tgcacatgga tttcttatta cacgccacag ccagacaacg gatgcaccac aatgcccaca
     5521 gggaacactt caggtctatg aaggcttttc tctcctgtat gtacaaggaa ataaaagagc
     5581 ccacggtcaa gacttgggga cggctggcag ctgccttcgt cgctttagta ccatgccttt
     5641 catgttctgc aacatcaata atgtttgcaa ctttgcttca agaaatgact attcttactg
     5701 gctctctacc ccagagccca tgccaatgag catgcaaccc ctaaagggcc agagcatcca
     5761 gccattcatt agtcgatgtg cagtatgtga agctccagct gtggtgatcg cagttcacag
     5821 tcagacgatc cagattcccc attgtcctca gggatgggat tctctgtgga ttggttattc
     5881 cttcatgatg catacaagtg caggggcaga aggctcaggt caagccctag cctcccctgg
     5941 ttcctgcttg gaagagtttc gttcagctcc cttcatcgaa tgtcatggga ggggtacctg
     6001 taactactat gccaactcct acagcttttg gctggcaact gtagatgtgt cagacatgtt
     6061 cagtaaacct cagtcagaaa cgctgaaagc aggagacttg aggacacgaa ttagccgatg
     6121 tcaagtgtgc atgaagagga cattctcgag cggcgtcttc acactcgaag atttcgttgg
     6181 ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc
     6241 cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg
     6301 tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga
     6361 ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt
     6421 taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga
     6481 ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac
     6541 agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc
     6601 cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct
     6661 ggcgtaaggc cgcgactcta gggcatgcaa gctgatccgg ctgctaacaa agcccgaaag
     6721 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcggcc
     6781 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6841 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6901 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6961 ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcgaatt
     7021 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     7081 cggtatttca caccgcatac gcggatctgc gcagcaccat ggcctgaaat aacctctgaa
     7141 agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     7201 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7261 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7321 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7381 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7441 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7501 ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgaagaag
     7561 cccgaactca ccgctaccag cgttgaaaaa tttctcatcg agaagttcga cagtgtgagc
     7621 gacctgatgc agttgtcgga gggcgaagag agccgagcct tcagcttcga tgtcggcgga
     7681 cgcggctatg tactgcgggt gaatagctgc gctgatggct tctacaaaga ccgctacgtg
     7741 taccgccact tcgccagcgc tgcactaccc atccccgaag tgttggacat cggcgagttc
     7801 agcgagagcc tgacatactg catcagtaga cgcgcccaag gcgttactct ccaagacctc
     7861 cccgaaacag agctgcctgc tgtgttacag cctgtcgccg aagctatgga tgctattgcc
     7921 gccgccgacc tcagtcaaac cagcggcttc ggcccattcg ggccccaagg catcggccag
     7981 tacacaacct ggcgggattt catttgcgcc attgctgatc cccatgtcta ccactggcag
     8041 accgtgatgg acgacaccgt gtccgccagc gtagctcaag ccctggacga actgatgctg
     8101 tgggccgaag actgtcccga ggtgcgccac ctcgtccatg ccgacttcgg cagcaacaac
     8161 gtcctgaccg acaacggccg catcaccgcc gtaatcgact ggtccgaagc tatgttcggg
     8221 gacagtcagt acgaggtggc caacatcttc ttctggcggc cctggctggc ttgcatggag
     8281 cagcagactc gctacttcga gcgccggcat cccgagctgg ccggcagccc tcgtctgcga
     8341 gcctacatgc tgcgcatcgg cctggatcag ctctaccaga gcctcgtgga cggcaacttc
     8401 gacgatgctg cctgggctca aggccgctgc gatgccatcg tccgcagcgg ggccggcacc
     8461 gtcggtcgca cacaaatcgc tcgccggagc gcagccgtat ggaccgacgg ctgcgtcgag
     8521 gtgctggccg acagcggcaa ccgccggccc agtacacgac cgcgcgctaa ggaggtaggt
     8581 cgagtttaga ctctagccat cacgatggcc gcaataaaat atctttattt tcattacatc
     8641 tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt
     8701 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     8761 tggctttcta cgtaatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8821 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8881 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8941 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9001 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9061 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9121 tgagcacttt caaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9181 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9241 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9301 tgagtgataa cactgcggcc aacttacttc tgacaactat cggaggaccg aaggagctaa
     9361 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9421 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9481 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9541 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9601 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9661 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9721 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9781 aattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat
     9841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10381 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    10501 gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga
    10561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10621 cttcacctag atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc
    10681 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc
    10741 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca
    10801 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg
    10861 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag
    10921 ccatcgagat aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg atcgagatct
//
