LOCUS       sequence-387883-       15402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pcoCasphi driven by UBQ10 gene 
            promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by 
            ribozymes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 15402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: 
            pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10
FEATURES             Location/Qualifiers
     source          1..15402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..26)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(23..45)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4195..4214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4448..4465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4480..4620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4546..4565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4706..4728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4964..5158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5224..6297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6726..7355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8941..9189)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           complement(9199..9266)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(9323..9359)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     promoter        complement(9393..9737)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          13138..13326
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             14645..14665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14672..14692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14711..14758
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2821 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2881 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2941 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3001 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3061 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3121 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3181 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3241 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3301 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3361 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3421 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3481 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3541 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3601 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4501 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4621 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4681 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4741 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4801 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4861 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4921 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4981 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     5041 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     5101 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     5161 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     5221 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     5281 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     5341 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     5401 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5461 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5521 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5581 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5641 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5701 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5761 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5821 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5881 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5941 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     6001 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     6061 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     6121 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     6181 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     6241 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta gatcactagt
     8941 cttatcttta atcatattcc atagtccata ccatagcaca tacagtagtt atatgctgca
     9001 gaagagatcc aacaaaacat tcacaatgga ttatagaaac atttgtttat tcattataat
     9061 gagatcttac attcatttaa tattagaaaa agccacaaat tcataacaca acaagccaag
     9121 aaaaaaacac aaacttaagc acacaagctt tttatttgac acaccaaata tttcatcttc
     9181 atcttcatat ttaattaagt cccattcgcc atgccgaagc atgttgccca gccggcgcca
     9241 gcgaggaggc tgggaccatg ccggccattg gctggattgt caactggtcc cctcgtgagg
     9301 ggcaatcgtt gagcgttccg acgacgagct tactcgtttc gtcctcacgg actcatcagg
     9361 tcggagctga ggcgggatcc tctagagtcg aggtcctctc caaatgaaat gaacttcctt
     9421 atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg
     9481 agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     9541 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga
     9601 tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct
     9661 tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc
     9721 tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg atatttttgg
     9781 agtagacaag tgtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg
     9841 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     9901 cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt
     9961 aggagccacc ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga
    10021 atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt
    10081 ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc accatgttga
    10141 cctgcaggca tgcaagcttg gcactggcct agtatcctag gaaggtacca gtctagctca
    10201 acagagcttt taacccaaat tggtacaata gaatacaact ttagatcata attctcaaaa
    10261 gaaagagatt ccttagctat tctatctgcc actccatttc cttctcggct tgtatgcaca
    10321 agcataaaat cctcaaactt gctaagtaga tactttatgt cttggataat tggattgaga
    10381 cttgacaagc ataactttca tgtaaccaaa gacacaagtt gctgagaatc cacctcaaaa
    10441 atgatcttcc tataattgaa tcgggataat gacagcacag cccatctaag agcctccact
    10501 tctacttcca gcacgcttct tacttttacc acagctcttg cacctaacca taacaccttc
    10561 cctgtatgat cgcgaagcac ccaccctaag ccacatttta atccttctgt tggccatgcc
    10621 ccatcaaagt tgcacttaac ccaagattgt ggtggagctt cccatgtttc tcgtctgtcc
    10681 cgacggtgtt gtggttggtg ctttccttac attctgagcc tctttccttc taatccactc
    10741 atctgcatct tcttgtgtcc ttactaatac ctcattggtt ccaaattccc tccctttaag
    10801 caccagctcg tttctgttct tccacagcct cccaagtatc caagggacta aagcctccac
    10861 attcttcaga tcaggatatt cttgtttaag atgttgaact ctatggaggt ttgtatgaac
    10921 tgatgatcta ggaccggata agttcccttc ttcatagcga acttattcaa agaatgtttt
    10981 gtgtatcatt cttgttacat tgttattaat gaaaaaatat tattggtcat tggactgaac
    11041 acgagtgtta aatatggacc aggccccaaa taagatccat tgatatatga attaaataac
    11101 aagaataaat cgagtcacca aaccacttgc cttttttaac gagacttgtt caccaacttg
    11161 atacaaaagt cattatccta tgcaaatcaa taatcataca aaaatatcca ataacactaa
    11221 aaaattaaaa gaaatggata atttcacaat atgttatacg ataaagaagt tacttttcca
    11281 agaaattcac tgattttata agcccacttg cattagataa atggcaaaaa aaaacaaaaa
    11341 ggaaaagaaa taaagcacga agaattctag aaaatacgaa atacgcttca atgcagtggg
    11401 acccacggtt caattattgc caattttcag ctccaccgta tatttaaaaa ataaaacgat
    11461 aatgctaaaa aaatataaat cgtaacgatc gttaaatctc aacggctgga tcttatgacg
    11521 accgttagaa attgtggttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc
    11581 cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa
    11641 taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt
    11701 attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc
    11761 ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca
    11821 atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc
    11881 tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata
    11941 tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt
    12001 tagatatcat cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa
    12061 tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag
    12121 tttctagttt gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagatgcc
    12181 gaagcccgcc gtcgaatcag agttttccaa agtcctcaag aaacactttc ctggggagcg
    12241 ttttaggtct agctatatga agaggggggg taaaattctg gcagcacaag gcgaggaagc
    12301 tgtagtggcg tacttgcagg gaaagagtga ggaggaaccg ccgaattttc agccgccggc
    12361 gaagtgccac gtggtcacca aaagcaggga tttcgcagaa tggcccataa tgaaagcctc
    12421 tgaagccata cagaggtaca tctatgcgct cagcactaca gagcgagctg cctgcaaacc
    12481 gggtaagagc tcagaaagtc acgcggcctg gttcgcggct acaggggtga gcaatcacgg
    12541 ctattctcat gtacaaggtc ttaacctgat ctttgaccac acgctaggac gatacgatgg
    12601 cgttttaaag aaagtacagc ttcgaaatga gaaggcccga gctagattgg aaagcataaa
    12661 cgcctcacga gctgatgaag gattgccgga aatcaaggcc gaggaggagg aggtagctac
    12721 aaatgaaaca ggtcatctac tacagccgcc aggcataaac ccatcattct acgtctacca
    12781 gaccatatct ccgcaggctt accgaccaag ggacgaaata gtgttaccac ccgagtacgc
    12841 cggttacgtc agggatccga acgctccgat tccactgggc gtggtcagga accgttgtga
    12901 catacagaag ggttgcccgg gatatatacc cgagtggcag agggaagctg gtacggcaat
    12961 tagtcccaag acaggaaaag cagtgacggt tccaggactt agcccgaaga agaataaacg
    13021 tatgcgtagg tactggaggt cagaaaagga gaaggctcaa gatgcacttc tcgtaactgt
    13081 aaggataggt accgattggg tagtcataga cgtaagagga ttactaagaa acgccaggta
    13141 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
    13201 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
    13261 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
    13321 gtgcaggtgg aggactattg ctcccaagga cataagttta aatgcacttc tagatttatt
    13381 taccggtgat ccagtcatcg atgtcagacg aaacatcgtg accttcacct ataccttgga
    13441 cgcttgcgga acttatgcta gaaaatggac tctcaaggga aaacagacaa aagcaacctt
    13501 agataaactg acagcgacac aaactgtggc cttagttgct atagatctgg gacaaacaaa
    13561 cccaattagc gcgggtatca gtcgtgtcac acaggagaac ggggccctcc agtgcgaacc
    13621 gcttgatcgt tttacattgc ctgatgacct tttgaaagat atttctgcgt accgaattgc
    13681 atgggaccgt aacgaggagg aactcagggc cagatccgtt gaggcactcc cagaggcaca
    13741 acaagcagag gtcagagctc tggacggggt ctccaaagag accgcgcgta cacagttgtg
    13801 cgcggacttc ggtctggacc caaagcgact accgtgggat aaaatgagta gcaataccac
    13861 gttcataagc gaggcgctcc tttccaacag cgtatcccgt gaccaagtat tctttacccc
    13921 ggccccaaag aaaggagcca agaagaaagc accggtggaa gtgatgcgaa aagacaggac
    13981 atgggcgcga gcgtacaaac cacgactctc agtagaagca caaaagttga aaaatgaggc
    14041 tctttgggct ttgaagcgta cctctccaga atatctaaag ttgtcacgac gtaaagaaga
    14101 attgtgtagg aggtccatta attacgtgat agaaaaaact agaaggcgaa cccaatgcca
    14161 aattgtgatc cccgttatag aagatctaaa tgtccgattt ttccacgggt ctggcaaacg
    14221 actcccgggc tgggataact tcttcacggc aaagaaggaa aaccgatggt ttatccaagg
    14281 gctacataag gcgttttctg acttaaggac ccaccgatcc ttctacgtgt tcgaggtgcg
    14341 tccagagaga acatcaatca cctgtccgaa atgcgggcac tgtgaagtgg ggaacaggga
    14401 tggagaagcc tttcagtgtc tcagttgcgg taagacatgc aacgcagatc tggacgtagc
    14461 gacacataac ttgactcaag tggcgctcac cggcaaaaca atgccgaaga gagaggagcc
    14521 tagagatgca caagggacag cccccgcgag aaagacgaag aaagctagca agtcaaaggc
    14581 acccccggct gaacgtgagg atcagactcc cgctcaagag ccgtcccaaa cgtccggatc
    14641 cggaccgaag aaaaagcgaa aggtagagga tcctaaaaag aagcgtaaag tctccttggg
    14701 ttctggctcc gactataagg atgacgatga caaagactat aaggatgacg acgacaagtg
    14761 aagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
    14821 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt
    14881 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
    14941 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
    15001 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
    15061 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
    15121 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
    15181 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
    15241 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
    15301 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
    15361 ttaatgcatt ttatgacttg ccaattgatt gacaacgaat tc
//
