LOCUS       sequence-387887-       14195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pcoCasphi driven by UBQ10 gene 
            promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at 
            the C-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: pC1300_pUB10_pcoCASphi_E9t_MCS_version1
FEATURES             Location/Qualifiers
     source          1..14195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..25)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(22..44)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    33..49
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(57..63,64..81,82..87))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    102..123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        314..990
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     927..947
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1057..2082
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2123..2297
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2375..2399
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2824..3618
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3705..4293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4194..4213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4447..4464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4479..4619
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4545..4564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4705..4727
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4963..5157
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5223..6296)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6725..7354)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8655..8679
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8867..8889
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8881..8898
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8882..8898
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10945..10992
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             10999..11019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          12006..12194
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             13504..13551
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
       61 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca
      241 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      301 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      361 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
      421 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
      481 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
      541 cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt ctactccaag
      601 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
      661 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
      721 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
      781 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      841 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
      901 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      961 agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac aaatctatct
     1021 ctctcgagct ttcgcagatc cggggggcaa tgagatatga aaaagcctga actcaccgcg
     1081 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     1141 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     1201 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     1261 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc
     1321 tattgcatct cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     1381 cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc
     1441 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     1501 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     1561 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     1621 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     1681 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     1741 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     1801 ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc
     1861 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     1921 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     1981 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     2041 ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agagtagatg ccgaccggga
     2101 tctgtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2161 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     2221 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     2281 gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc
     2341 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     2401 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     2461 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     2521 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     2581 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg
     2641 cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
     2701 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
     2761 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     2821 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     2881 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     2941 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     3001 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     3061 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     3121 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     3181 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     3241 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     3301 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     3361 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     3421 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     3481 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     3541 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     3601 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
     3661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3721 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3781 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3841 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3901 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3961 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4021 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4081 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4141 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4201 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4261 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4321 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4381 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4441 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4501 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     4561 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     4621 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4681 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4741 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     4801 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     4861 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     4921 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     4981 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     5041 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     5101 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     5161 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     5221 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     5281 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     5341 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     5401 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     5461 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     5521 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     5581 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     5641 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     5701 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     5761 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     5821 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     5881 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     5941 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     6001 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     6061 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     6121 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     6181 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     6241 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     6301 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
     6361 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     6421 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     6481 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     6541 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     6601 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     6661 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     6721 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     6781 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     6841 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     6901 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     6961 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     7021 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     7081 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     7141 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     7201 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     7261 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     7321 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     7381 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     7441 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     7501 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     7561 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     7621 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     7681 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     7741 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     7801 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     7861 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     7921 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     7981 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     8041 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     8101 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     8161 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     8221 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     8281 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     8341 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     8401 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     8461 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     8521 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     8581 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
     8641 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga
     8701 aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc attcaggctg
     8761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8881 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcgactctag atcactagta
     8941 tcctaggaag gtaccagtct agctcaacag agcttttaac ccaaattggt acaatagaat
     9001 acaactttag atcataattc tcaaaagaaa gagattcctt agctattcta tctgccactc
     9061 catttccttc tcggcttgta tgcacaagca taaaatcctc aaacttgcta agtagatact
     9121 ttatgtcttg gataattgga ttgagacttg acaagcataa ctttcatgta accaaagaca
     9181 caagttgctg agaatccacc tcaaaaatga tcttcctata attgaatcgg gataatgaca
     9241 gcacagccca tctaagagcc tccacttcta cttccagcac gcttcttact tttaccacag
     9301 ctcttgcacc taaccataac accttccctg tatgatcgcg aagcacccac cctaagccac
     9361 attttaatcc ttctgttggc catgccccat caaagttgca cttaacccaa gattgtggtg
     9421 gagcttccca tgtttctcgt ctgtcccgac ggtgttgtgg ttggtgcttt ccttacattc
     9481 tgagcctctt tccttctaat ccactcatct gcatcttctt gtgtccttac taatacctca
     9541 ttggttccaa attccctccc tttaagcacc agctcgtttc tgttcttcca cagcctccca
     9601 agtatccaag ggactaaagc ctccacattc ttcagatcag gatattcttg tttaagatgt
     9661 tgaactctat ggaggtttgt atgaactgat gatctaggac cggataagtt cccttcttca
     9721 tagcgaactt attcaaagaa tgttttgtgt atcattcttg ttacattgtt attaatgaaa
     9781 aaatattatt ggtcattgga ctgaacacga gtgttaaata tggaccaggc cccaaataag
     9841 atccattgat atatgaatta aataacaaga ataaatcgag tcaccaaacc acttgccttt
     9901 tttaacgaga cttgttcacc aacttgatac aaaagtcatt atcctatgca aatcaataat
     9961 catacaaaaa tatccaataa cactaaaaaa ttaaaagaaa tggataattt cacaatatgt
    10021 tatacgataa agaagttact tttccaagaa attcactgat tttataagcc cacttgcatt
    10081 agataaatgg caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa
    10141 tacgaaatac gcttcaatgc agtgggaccc acggttcaat tattgccaat tttcagctcc
    10201 accgtatatt taaaaaataa aacgataatg ctaaaaaaat ataaatcgta acgatcgtta
    10261 aatctcaacg gctggatctt atgacgaccg ttagaaattg tggttgtcga cgagtcagta
    10321 ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc
    10381 acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa
    10441 acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct
    10501 cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc
    10561 tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc
    10621 tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg
    10681 ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc
    10741 gaagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat
    10801 agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg
    10861 tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtcgattaat
    10921 ctgagttttt ctgattaaca gatggattac aaggatgatg atgataagga ttacaaggat
    10981 gatgatgata agatggctcc aaagaagaag agaaaggttg gaatccacgg agttccagct
    11041 gctatgccga agcccgccgt cgaatcagag ttttccaaag tcctcaagaa acactttcct
    11101 ggggagcgtt ttaggtctag ctatatgaag agggggggta aaattctggc agcacaaggc
    11161 gaggaagctg tagtggcgta cttgcaggga aagagtgagg aggaaccgcc gaattttcag
    11221 ccgccggcga agtgccacgt ggtcaccaaa agcagggatt tcgcagaatg gcccataatg
    11281 aaagcctctg aagccataca gaggtacatc tatgcgctca gcactacaga gcgagctgcc
    11341 tgcaaaccgg gtaagagctc agaaagtcac gcggcctggt tcgcggctac aggggtgagc
    11401 aatcacggct attctcatgt acaaggtctt aacctgatct ttgaccacac gctaggacga
    11461 tacgatggcg ttttaaagaa agtacagctt cgaaatgaga aggcccgagc tagattggaa
    11521 agcataaacg cctcacgagc tgatgaagga ttgccggaaa tcaaggccga ggaggaggag
    11581 gtagctacaa atgaaacagg tcatctacta cagccgccag gcataaaccc atcattctac
    11641 gtctaccaga ccatatctcc gcaggcttac cgaccaaggg acgaaatagt gttaccaccc
    11701 gagtacgccg gttacgtcag ggatccgaac gctccgattc cactgggcgt ggtcaggaac
    11761 cgttgtgaca tacagaaggg ttgcccggga tatatacccg agtggcagag ggaagctggt
    11821 acggcaatta gtcccaagac aggaaaagca gtgacggttc caggacttag cccgaagaag
    11881 aataaacgta tgcgtaggta ctggaggtca gaaaaggaga aggctcaaga tgcacttctc
    11941 gtaactgtaa ggataggtac cgattgggta gtcatagacg taagaggatt actaagaaac
    12001 gccaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag
    12061 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt
    12121 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat
    12181 ttgttgatgt gcaggtggag gactattgct cccaaggaca taagtttaaa tgcacttcta
    12241 gatttattta ccggtgatcc agtcatcgat gtcagacgaa acatcgtgac cttcacctat
    12301 accttggacg cttgcggaac ttatgctaga aaatggactc tcaagggaaa acagacaaaa
    12361 gcaaccttag ataaactgac agcgacacaa actgtggcct tagttgctat agatctggga
    12421 caaacaaacc caattagcgc gggtatcagt cgtgtcacac aggagaacgg ggccctccag
    12481 tgcgaaccgc ttgatcgttt tacattgcct gatgaccttt tgaaagatat ttctgcgtac
    12541 cgaattgcat gggaccgtaa cgaggaggaa ctcagggcca gatccgttga ggcactccca
    12601 gaggcacaac aagcagaggt cagagctctg gacggggtct ccaaagagac cgcgcgtaca
    12661 cagttgtgcg cggacttcgg tctggaccca aagcgactac cgtgggataa aatgagtagc
    12721 aataccacgt tcataagcga ggcgctcctt tccaacagcg tatcccgtga ccaagtattc
    12781 tttaccccgg ccccaaagaa aggagccaag aagaaagcac cggtggaagt gatgcgaaaa
    12841 gacaggacat gggcgcgagc gtacaaacca cgactctcag tagaagcaca aaagttgaaa
    12901 aatgaggctc tttgggcttt gaagcgtacc tctccagaat atctaaagtt gtcacgacgt
    12961 aaagaagaat tgtgtaggag gtccattaat tacgtgatag aaaaaactag aaggcgaacc
    13021 caatgccaaa ttgtgatccc cgttatagaa gatctaaatg tccgattttt ccacgggtct
    13081 ggcaaacgac tcccgggctg ggataacttc ttcacggcaa agaaggaaaa ccgatggttt
    13141 atccaagggc tacataaggc gttttctgac ttaaggaccc accgatcctt ctacgtgttc
    13201 gaggtgcgtc cagagagaac atcaatcacc tgtccgaaat gcgggcactg tgaagtgggg
    13261 aacagggatg gagaagcctt tcagtgtctc agttgcggta agacatgcaa cgcagatctg
    13321 gacgtagcga cacataactt gactcaagtg gcgctcaccg gcaaaacaat gccgaagaga
    13381 gaggagccta gagatgcaca agggacagcc cccgcgagaa agacgaagaa agctagcaag
    13441 tcaaaggcac ccccggctga acgtgaggat cagactcccg ctcaagagcc gtcccaaacg
    13501 tccaagagac cagctgctac caagaaggct ggacaggcta agaagaagaa gtgaagagct
    13561 ttcgttcgta tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg
    13621 cacacaccag aatcctactg agtttgagta ttatggcatt gggaaaactg tttttcttgt
    13681 accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg
    13741 aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat
    13801 taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat
    13861 gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa
    13921 tatgaggagt aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata
    13981 ttttcagacc tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta
    14041 gacatttatg aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg
    14101 ctttataatt atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc
    14161 attttatgac ttgccaattg attgacaacg aattc
//
