LOCUS       sequence-387895-       10483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second-generation mammalian acoustic reporter gene derived from 
            Anabaena.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Hurt RC, Buss MT, Duan M, Wong K, You MY, Sawyer DP, Swift MB, Dutka
            P, Barturen-Larrea P, Mittelstein DR, Jin Z, Abedi MH, Farhadi A, 
            Deshpande R, Shapiro MG
  TITLE     Genomically mined acoustic reporter genes for real-time in vivo 
            monitoring of tumors and tumor-homing bacteria.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01581-y.
  PUBMED    36593411
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01581-y."
COMMENT     Sequence Label: mARGAna (cassette 2, transient)
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        778..1081
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1083..1285
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1236..1256
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1369..1378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2401..2457
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3220..3276
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3760..3816
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4570..4626
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5002..5058
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5755..5811
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6172..6228
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6535..6554)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(7036..7055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7037..7055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7450..8038
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7503..7523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7921..7932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8058..8534
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8257..8276)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      8670..9124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8757..8776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8966..8987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(9411..9479,9480..10271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9629..9648)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    10373..10406
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttattcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc ccatatatgg agttccgcgt
      781 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      841 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      901 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      961 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1021 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1081 gctgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1141 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1201 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1261 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatcagat ctttgtcgat
     1321 cctaccatcc actcgacaca cccgccagcg gccgctgcga gggtctccgc caccatggtg
     1381 accaccacca aagtgaacca caagcgggcc gtgctgagac tgaggcctgg ccagtttgtc
     1441 gtgacccccg ccattgagcg ggtggccatt agagccctga gatacctgaa gtccggcttc
     1501 cccgtgcacc tgagaggacc tgccggaacc ggcaagacca cactggccat gcacctggcc
     1561 aactgcctgg acagacccgt gatgctgctg ttcggcgacg accagttcaa gagcagcgac
     1621 ctgatcggca gcgagagcgg ctacacccac aagaaggtgc tggacaacta catccacagc
     1681 gtcgtgaagc tggaagatga gttcaagcag aactgggtgg acagcagact gaccctggcc
     1741 tgccgggaag gcttcaccct ggtgtacgac gagttcaacc ggtccagacc cgaagtgaac
     1801 aacgtgctgc tgagcgccct ggaagagaag atcctgagcc tgccccccag cagcaaccag
     1861 ccagagtacc tgagcgtgaa cccccagttc agagtgatct tcaccagcaa ccccgaggaa
     1921 tatgccggcg tgcacagcac ccaggacgcc ctgatggacc ggctcgtgac catctccatg
     1981 cccgagcccg atgagatcac ccagaccgag atcctgatcc agaaaacaaa catcgaccgc
     2041 gagagcgcca acttcatcgt gcggctcgtg aagtccttca gactggccac aggcgccgag
     2101 aaaaccagcg gcctgagaag ctgcctgatg atcgccaaag tgtgcgccga caacaacatc
     2161 cccgtgacca ccgagagcct ggacttcccc gatatcgcca tcgacatcct gttcaaccgc
     2221 agccacctga gcatgagcga gtccaccaac atctttctgg aactgctgga taagttcagc
     2281 gccgaggaac tggaaatcct gaacaacaga gtgaccggcg acaacgactt cctgatcgac
     2341 aacagccagt tcgtgtccca gcagctggcc ggacagccca acggagcgcc aggttccggg
     2401 gctactaact tcagcctcct taaacaggct ggcgatgtgg aagaaaatcc cggaccagtg
     2461 ctgcccacca gaccccagac caacagcagc cggaccatca acaccagcac ccagggcagc
     2521 accctggccg acatcctgga aagagtgctg gacaagggca tcgtgatcgc cggggacatc
     2581 agcatctcta tcgccagcac cgagctggtg cacatccgga tcagactgct gatctccagc
     2641 gtggacaagg ccaaagagat gggcatcaac tggtgggaga gcgaccccta cctgagcacc
     2701 aaggcccaga gactggtgga agagaaccag cagctccagc acagactgga aagcctggaa
     2761 gccaagctga acagcctgac cagcagcagc gtgaaagagg aaatccccct ggccgccgac
     2821 gtgaaggacg acctgtatca gaccagcgcc aagatcccca gccccgtgga tacccctatc
     2881 gaggtgctgg acttccaggc ccagtctagc ggcggcacac ccccttacgt gaacacctct
     2941 atggaaatac tggattttca ggctcagacc tccgccgaga gcagcagccc tgtgggctct
     3001 accgtggaaa tactggactt ccaggcacag acaagcgagg aatccagctc ccccgtggtg
     3061 tccacagtgg aaatactgga tttccaggcc cagacttctg aagagtccag ctctccagtg
     3121 ggaagcactg tggaaatact ggactttcag gcccagacca gtgaagagat ccccagctct
     3181 gtggaccccg ccatcgatgt gggtgccccg ggatctggcg caacaaattt tagtctcctc
     3241 aagcaggcag gagatgtcga ggaaaaccct ggacccgtgg tgtgcacccc tgccgagaac
     3301 ttcaacaaca gcctgacaat cgccagcaag cccaagaacg aggccggact ggctcctctg
     3361 ctgctgaccg tgctggaact cgtgcggcag ctgatggaag cccaagtgat ccggcggatg
     3421 gaagaggacc tgctgagcga gcccgacctg gaaagagccg ccgatagcct ccagaagctg
     3481 gaagaacaga tcctgcacct gtgcgagatg ttcgaggtgg accccgccga cctgaacatc
     3541 aacctgggcg agatcggcac cctgctgcct agcagcggca gctactatcc tggccagcca
     3601 agcagcagac cctctgtgct ggaactgctg gaccggctgc tgaacaccgg catcgtggtg
     3661 gacggcgaga ttgacctggg aatcgcccag atcgacctga tccacgccaa gctgagactg
     3721 gtgctgacct ccaagcccat cggggcacct ggctcaggag cgaccaactt ctcattgctc
     3781 aaacaagccg gagatgttga ggagaatcca ggccctgtga gcatccccct gtacctgtac
     3841 ggcatcttcc ccaacaccat ccccgagaca ctggaactgg aaggcctgga caagcagccc
     3901 gtgcacagcc aggtggtgga cgagttctgc ttcctgtaca gcgaggcccg gcaggaaaag
     3961 tacctggcca gcagacggaa cctgctgacc cacgagaagg tgctggaaca gaccatgcac
     4021 gccggcttca gagtgctgct gcccctgaga tttggcctgg tcgtgaagga ctgggagaca
     4081 atcatgagcc agctgatcaa cccccacaag gaccagctga accagctgtt ccagaagctg
     4141 gccggcaagc gggaagtgtc catcaagatt ttctgggacg ccaaggccga gcttcagacc
     4201 atgatggaaa gccaccagga cctgaagcag cagcgggaca acatggaagg caagaaactg
     4261 agcatggaag aagtgatcca gatcgggcag ctgatcgaga tcaacctgct ggcccggaag
     4321 caggccgtga tcgaggtgtt cagccaggaa ctgaacccct tcgcccagga aatcgtggtg
     4381 tccgacccca tgaccgagga aatgatctac aacgccgcct tcctgatccc ctgggagagc
     4441 gagagcgagt tcagcgagcg ggtggaagtg atcgaccaga agttcggcga ccggctgcgg
     4501 atcagataca acaacttcac cgccccctac acctttgccc agctggactc tggcgctccc
     4561 gggtcaggtg ccactaattt ttcattgttg aagcaagctg gggatgttga agagaaccca
     4621 gggcctgtgc tgaccaaact cctgctgctg cccatcatgg gacccctgaa tggcgtcgtg
     4681 tggatcgccg agcagatcca ggaacggacc aacaccgagt tcgacgccca ggaaaacctg
     4741 cacaaacagc tgctgagcct ccagctgagc ttcgacatcg gcgagatcgg agaggaagag
     4801 ttcgagattc aggaagagga aatcctgctg aagatccagg ccctggaaga agaggcccgg
     4861 ctggaactgg aagccgagca ggaagaagcc agactggaac tggaagctga acaggaagat
     4921 ttcgagtacc caccacagtt caccgccgaa gtgaacaagg atcagcatct ggtgctgctg
     4981 cccggtgccc cgggatctgg cgctactaac ttcagcctcc ttaaacaggc tggcgatgtg
     5041 gaagaaaatc ccggaccagt ggaactggaa aacctgtaca cctacgcctt tctggaaatc
     5101 cccagcagcc ccctgatcct gcctcagggc gctgctaatc aggtggtgct gatcaacggc
     5161 accgagctgg ccgccatcgt ggaacctggc atcttcctgg aatccttcca gaacaacgac
     5221 gagaagatca tccagatggc cctgagccac gacagagtga tctgcgagct gttccagcag
     5281 atcaccgtgc tgcccctgag attcggcacc tacttcacca gcaccaacaa cctgctgaac
     5341 cacctgaagt cccacgagaa agagtaccag aacaagctgg aaaagatcaa cgggaagaac
     5401 gagttcaccc tgaagctgat cccccggatg atcgaggaaa tcgtgccctc tgagggcgga
     5461 ggcaaggact acttcctggc caagaagcag cgctaccaga atcagaacaa cttctctatc
     5521 gcccaggccg ccgagaagca gaacctgatc gacctgatca ccaaagtgaa ccagctgccc
     5581 gtggtggtgc aggaacagga agaacagatc caaatctacc tgctggtgtc ctgccaggat
     5641 aagaccctgc tgctggaaca gttcctgacc tggcagaaag cctgccccaa gtgggatctg
     5701 ctgctgggcg actgcctgcc cccctaccac tttatcggag cgccaggttc cggggcaaca
     5761 aattttagtc tcctcaagca ggcaggagat gtcgaggaaa accctggacc cgtgatcaag
     5821 aacatccagg tgttctttat gaaaaccatc agcaaccggt ccatcagccg ggccaagatc
     5881 agcaccatgc ccagacctaa gagcgacgcc agcagccagc tggacctgta caagatggtc
     5941 accgagaagc agagaatcca gcgggatatg tacagcatca aagaacggat gggcctgctc
     6001 cagcagcggc tggacatcct gaaccagcag atcgaggcca ccgagaaaac catccacaag
     6061 ctgcggcagc cccacagcaa caccgcccag aatatcgtgc ggagcaacat cttcgtggaa
     6121 agcaacaact accagacctt cgaggtggaa tacggggcac ctggctcagg agccactaat
     6181 ttttcattgt tgaagcaagc tggggatgtt gaagagaacc cagggcctgt ggtgtccaag
     6241 ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac
     6301 ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc
     6361 ctgaagttca tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca
     6421 ctgacctacg gcgtgcagtg cttcgccaga taccccgacc acatgaagca gcacgatttc
     6481 ttcaagagcg ccatgcccga gggctacgtg caggaacgga ccatcttctt caaggacgac
     6541 ggcaactaca agacaagagc cgaagtgaag ttcgagggcg acaccctcgt gaaccggatc
     6601 gagctgaagg gcatcgactt caaagaggat ggcaacatcc tgggccacaa gctggagtac
     6661 aactacaaca gccacaaggt gtacatcacc gccgacaagc agaaaaacgg catcaaagtg
     6721 aacttcaaga cccggcacaa catcgaggac ggcagcgtgc agctggccga ccactaccag
     6781 cagaacaccc ccatcggaga tggccccgtg ctgctgcccg acaaccacta cctgagcaca
     6841 caaagcgccc tgagcaagga ccccaacgag aagcgggacc acatggtgct gctggaattt
     6901 gtgaccgccg ctggcatcac cctgggcatg gacgagctgt acaagtgact cgagtctaga
     6961 gggccccgtg gctgtaatct agaggatccc tcgaggggcc caagcttacg cgtgcatgcg
     7021 acgtcatagc tctctcccta tagtgagtcg tattataagc tagcttggga tctttgtgaa
     7081 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     7141 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctagctgcat atgcttgctg
     7201 cttgagagtt ttgcttactg agtatgattt atgaaaatat tatacacagg agctagtgat
     7261 tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc
     7321 tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7381 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7441 tcgtggctga atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7501 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7561 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7621 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7681 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7741 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7801 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7861 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7921 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7981 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcca
     8041 gagacaatta acttcgcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     8101 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     8161 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     8221 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     8281 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     8341 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     8401 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     8461 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     8521 ccttccctgt ccttgaagtt ctcagatcct gcattaatga atcggccaac gcgcggggag
     8581 aggcggtttg cgtattggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8641 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8701 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8761 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8821 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8881 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8941 gacgttggag tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac
     9001 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9061 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9121 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9181 tacattcaaa tatgtatccg ctcatgccag gtcttggact ggtgagaacg gcttgctcgg
     9241 cagcttcgat gtgtgctgga gggagaataa aggtctaaga tgtgcgatag agggaagtcg
     9301 cattgaatta tgtgctgtgt agggatcgct ggtatcaaat atgtgtgccc acccctggca
     9361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg ccataacttc
    10381 gtataatgta tgctatacga agttatggca tgaccaaaat cccttaacgt gagttttcgt
    10441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
