LOCUS       sequence-38794-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1312
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(248..266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        345..449
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(668..687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg
       61 accggtaatg caggcaaatt ttggtgtacg gtcagtaaat tggacatggt ggcggaagtt
      121 cctatacctt ttgaagaata ggaacttcgg aataggaact tcggcgcgcc acgcgtggcc
      181 ggcctaggag ctcgaattca ttaagggtta ttgaatatga tcggaattaa ttcttgaaga
      241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      301 tagacgtgcg gccgccgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      421 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      481 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      541 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      601 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      661 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      721 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      781 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      841 ctgcggccaa cttacttctg acaacgatcg gag
//
