LOCUS       sequence-387949-        2444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-4 
            beta-4 fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Mattera R, De Pace R, Bonifacino JS
  TITLE     The adaptor protein chaperone AAGAB stabilizes AP-4 complex 
            subunits.
  JOURNAL   Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17.
  PUBMED    35976721
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-10-1"; volume: "33(12):ar109. doi"; pages: " 
            10.1091/mbc.E22-05-0177. Epub 2022 Aug 17"
COMMENT     Sequence Label: pGADT7-AP-4 beta-4
FEATURES             Location/Qualifiers
     source          1..2444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             148..162
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agatctttaa tacgactcac
       61 tatagggcga gcgccgccat ggagtaccca tacgacgtac cagattacgc tcatatggcc
      121 atggaggcca gtgaattcca cccgggcgac gacgacgaca agatgccgta ccttggctcc
      181 gaggacgtgg tgaaggagct gaagaaggct ctgtgcaatc ctcacattca agctgatagg
      241 ctgcgctacc ggaatgtcat ccagcgagtg attaggtaca tgactcaagg cttggacatg
      301 tctggtgttt ttatggaaat ggtgaaggcc agtgccactg tagatattgt ccagaagaag
      361 ttggtttatc tgtacatgtg cacatatgct cccctgaaac cagatctggc tctcctggcc
      421 atcaatacgc tgtgcaaaga ctgctcagac cccaatccaa tggtgcgagg gctggcgtta
      481 cggagcatgt gtagcctcag gatgcctggt gtgcaggagt atatacaaca gcctattctc
      541 aatggtctgc gggataaggc ttcatatgtc aggagagtgg tagtccttgg atgtgccaag
      601 atgcataatc ttcatggaga ctctgaagta gatggtgccc tggtaaatga attatacagt
      661 ttgctgcgtg accaggatcc aattgtagtt gtgaactgct tgaggtctct agaggaaatt
      721 ctgaaacagg aaggaggcgt tgtcatcaat aagcccattg ctcaccatct cttaaatcga
      781 atgtcaaaac tggaccaatg gggccaggct gaagtattga actttctgct acgctaccaa
      841 ccccgcagtg aggaagaact atttgacatt ctcaatctgt tggatagttt cctcaagagc
      901 agtagcccag gtgtggtgat gggagctacc aaactttttc tgatcttggc aaaaatgttt
      961 ccccacgtac aaactgatgt ccttgtgcgg gtcaagggac ctttgctagc tgcctgttct
     1021 tcagagagcc gtgagctctg ttttgttgct ctttgtcatg tacgccagat cttgcatagt
     1081 ttaccaggtc actttagcag ccactacaaa aagttttttt gctcctactc ggagccccac
     1141 tacatcaaac tacagaaagt ggaggtgctg tgtgaactgg tgaacgatga gaatgtgcag
     1201 caggtgctag aggagcttcg agggtactgc acggatgtgt ctgcggactt tgcacaggct
     1261 gccatctttg ccataggtgg cattgccagg acttacacag atcaatgtgt tcagatttta
     1321 acagagttgc tgggtcttcg acaagagcac attaccacag tggtggtgca gactttccga
     1381 gacctggttt ggttgtgtcc tcagtgtact gaagctgtat gtcaggccct gcccggctgt
     1441 gaagagaaca ttcaagatag tgaggggaag caagcactta tttggctact tggtgtccat
     1501 ggggaaagaa ttcctaatgc tccttatgtg ttagaggact ttgttgagaa tgtgaagtcg
     1561 gaaacatttc cagctgttaa gatggagctg ctcactgctt cgctgcgcct tttcctctcc
     1621 cgacctgctg agtgccagga catgctagga cgtttgttgt attactgcat agaggaagaa
     1681 aaagatatgg ctgtacggga ccgaggtctc ttctattatc gcctcctctt agttggcatt
     1741 gatgaagtta agcggattct gtgtagccct aaatctgacc ctactcttgg acttttggag
     1801 gatccggcag aaagacctgt gaatagctgg gcctcagact tcaacacact ggtgccagtg
     1861 tatggcaaag cccactgggc aactatctct aaatgccagg gggcagagcg ttgtgaccca
     1921 gagcttccta aaacttcatc ctttgccgca tcaggaccct tgattcctga agagaacaag
     1981 gagagggtac aagaactccc tgattctgga gccctcatgc tagtccccaa tcgccagctt
     2041 actgctgatt attttgagaa aacttggctt agccttaaag ttgctcatca gcaagtgttg
     2101 ccttggcggg gagaattcca tcctgacacc ctccagatgg ctcttcaagt agtgaacatc
     2161 cagaccatcg caatgagtag ggctgggtct cggccatgga aagcatacct cagtgctcag
     2221 gatgatactg gctgtctgtt cttaacagaa ctgctattgg agcctggaaa ctcagaaatg
     2281 cagatctctg tgaaacaaaa tgaagcaaga acggagacgc tgaatagttt tatttctgta
     2341 ttagaaactg tgattggaac aattgaagaa ataaaatcat aacctgcagc caagcttgcg
     2401 gccgcactcg agctgcagat gaatcgtaga tactgaaaaa cccc
//
