LOCUS       sequence-387972-       11370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with estradiol inducible promoter: 
            p35S_XVE-PhiC31-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1P38
FEATURES             Location/Qualifiers
     source          1..11370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    256..278
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    286..462
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(694..1719)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1739..2084)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2287..2309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2301..2318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2302..2318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2325..2344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2325..2343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2459..2803
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2834..3085
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             3101..3334
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3344..4279
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3392..4285
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      4729..4980
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5301..5347
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5374..7191
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     misc_feature    7746..7770
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7861..8449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7941..7960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9958..10773)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9958..10773)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10681..10700
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10765..10784
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(11064..11370,1..129)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tatgttcaat tagcttttca attatgaata tatatatcaa ttattggtcg cccttggctt
       61 gtggacaatg cgctacgcgc accggctccg cccgtggaca accgcaagcg gttgcccacc
      121 gtcgagcgcc tttgcccaca acccggcggc cggccgcaac agatcgtttt ataaattttt
      181 ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc gggcttctgg atttccgatc
      241 cccggaatta gatcttggca ggatatattg tggtgtaacg ttatcgatct ggattttagt
      301 actggatttt ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa
      361 tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat
      421 ttcccttatc gggaaactac tcacacatta tttatggaga aaatagagag agatagattt
      481 gtagagagag actggtgatt tcagcgtacc gaattaattc ctgaatcact gcgaccggcc
      541 ctcccgcgac ccagccgagc gagcttagcg aactgtggac gagaactgtg ccaccaagcg
      601 taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc
      661 tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg gacgagtgct
      721 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
      781 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
      841 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
      901 ttggtcaaga ccaatgcgga gcatatacgc ccggagcctt ggcgatcctg caagctccgg
      961 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     1021 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     1081 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     1141 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     1201 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     1261 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     1321 gaacccgctc gtctggctaa gatcggccgc agcgatagca tccatagcct ccgcgaccgg
     1381 ctgaagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     1441 gcgggagatg caataggtca ggctctcgct gaactcccca atgtcaagca cttccggaat
     1501 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     1561 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     1621 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     1681 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata ggggggatca gcttgggctg
     1741 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     1801 attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgaagacg
     1861 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     1921 cactgtcggc agaggcatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
     1981 aggagccacc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga
     2041 atccgaggag gtttcccgaa attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     2101 ctgagactgt atctttgaca tttttggagt aggggtacga taacattaac gcttacaatt
     2161 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2281 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     2341 agggcttgga gccacgatac cggtggtacc catgcctgca ggtcaacatg gtggagcacg
     2401 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag agggctattg
     2461 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     2521 gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc catcattgcg
     2581 ataaaggaaa ggctatcgtt caagatgcct ctaccgacag tggtcccaaa gatggacccc
     2641 cacccacgag gaacatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     2701 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     2761 acccttcctc tatataagga agttcatttc atttggagag gacctcctcg agtacgtacc
     2821 aattccgggc ggaatgaaag cgttaacggc caggcaacaa gaggtgtttg atctcatccg
     2881 tgatcacatc agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg
     2941 gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat
     3001 tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg caggaagagg aagaagggtt
     3061 gccgctggta ggtcgtgtgg ctgccggtga accgtcgagc gcccccccga ccgatgtcag
     3121 cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct
     3181 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
     3241 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
     3301 taccgatgcc cttggaattg acgagtacgg tggggatccg tctgctggag acatgagagc
     3361 tgccaacctt tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt
     3421 gtccctgacg gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta
     3481 ttccgagtat gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa
     3541 cctggcagac agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt
     3601 ggatttgacc ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat
     3661 gattggtctc gtctggcgct ccatggagca cccagggaag ctactgtttg ctcctaactt
     3721 gctcttggac aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct
     3781 gctggctaca tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct
     3841 caaatctatt attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc
     3901 tctggaagag aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca
     3961 cctgatggcc aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct
     4021 cctcatcctc tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat
     4081 gaagtgcaag aacgtggtgc ccctctatga cctgctgctg gagatgctgg acgcccaccg
     4141 cctacatgcg cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt
     4201 ggccactgcg ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc
     4261 agagggtttc cctgccacag tctgagagct ccctggcgga attcccagag atgttagctg
     4321 aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaaaag cttctgtttc
     4381 atcaaaaatg actcgaccta actgagtaag ctagcttgtt cgagtattat ggcattggga
     4441 aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt
     4501 tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt
     4561 ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg
     4621 aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc
     4681 taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcat cgttcaaaca
     4741 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     4801 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     4861 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     4921 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     4981 gggaattgat cccccctcga cagcttgcat gccgcttggg ctgcaggtcg aggctaaaaa
     5041 actaatcgca ttatcatccc ctcgacgtac tgtacatata accactggtt ttatatacag
     5101 cagtactgta catataacca ctggttttat atacagcagt cgacgtactg tacatataac
     5161 cactggtttt atatacagca gtactgtaca tataaccact ggttttatat acagcagtcg
     5221 aggtaagatt agatatggat atgtatatgg atatgtatat ggtggtaatg ccatgtaata
     5281 tgctcgactc taggatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     5341 gagaggacac gctgaagcta gtcgactcta gcaatggaca cgtatgccgg cgcttatgat
     5401 agacaatcaa gagaaagaga gaactcttct gccgcctctc cagctacaca gagatctgcc
     5461 aacgaggata aagctgctga tttacaaaga gaggtagaga gagatggggg taggtttaga
     5521 tttgtgggtc atttctctga ggctcctggt acatcagcct ttggtactgc tgaacggcct
     5581 gaatttgaaa gaatattaaa tgagtgtcgg gccggtcgac taaatatgat aatagtttat
     5641 gatgtatcta ggttcagtag actcaaagtt atggatgcaa ttccgatcgt gagcgagcta
     5701 cttgcactcg gtgttactat cgtaagtaca caagaaggtg tattcaggca gggtaatgtg
     5761 atggacctta tacaccttat tatgcgactc gacgctagcc ataaagagtc atcactgaag
     5821 agtgctaaaa tcttggacac caagaacctg caaagggagc ttggaggcta tgtaggagga
     5881 aaagctccgt atggtttcga actcgtgagt gagacaaaag agattaccag aaatggtagg
     5941 atggtgaacg tggttattaa taagctagct catagcacaa cccctctcac agggcctttt
     6001 gagttcgaac ctgacgttat tagatggtgg tggagagaga tcaaaactca caaacatctt
     6061 ccatttaagc caggtagcca ggctgctatc caccctggat ctatcactgg gctttgtaaa
     6121 aggatggatg ctgacgcagt gccaactcga ggggaaacaa tcggtaagaa gactgcatct
     6181 tccgcttggg atcccgctac agttatgaga atccttcggg atccgcgtat tgctggattc
     6241 gccgcagaag tcatttataa gaaaaaacct gatggtactc ctactacaaa aatcgaaggt
     6301 tataggatcc aaagggatcc tattactttg cgtccagttg aattagactg tggaccgatc
     6361 atcgaacctg ccgagtggta cgagttgcag gcctggcttg atggtagagg gcgtggaaag
     6421 ggtttatctc ggggacaggc aattttgtca gcaatggata agctttattg tgagtgtggg
     6481 gctgttatga catcaaaacg tggagaagag tcaatcaagg attcttacag gtgcagacgt
     6541 cgtaaagtgg tggacccttc tgctccagga cagcatgagg gtacatgcaa cgtttctatg
     6601 gctgctcttg ataaatttgt ggccgaacgt attttcaaca agattagaca cgctgaaggg
     6661 gatgaggaaa ctttagctct tctttgggag gctgcccggc gttttggtaa gctaacagag
     6721 gcaccagaaa aaagtggcga aagagctaat ttggtagccg aaagagcaga cgccttgaat
     6781 gcattggaag aactttacga agatagggct gccggagcat atgatggacc agttggtcgg
     6841 aagcatttca ggaagcaaca agctgcacta actttgagac agcaaggcgc agaggagcga
     6901 ctcgcagagc ttgaggccgc cgaggcccca aagcttccac tcgatcaatg gtttcctgag
     6961 gatgccgacg ctgatcctac aggtccaaag agctggtggg gaagagctag cgtagacgat
     7021 aagagagtgt ttgtgggtct atttgttgac aagattgtag ttactaagtc cactacgggt
     7081 cgaggacagg gcactcccat cgaaaagaga gccagtatta cctgggcaaa gcctccaacc
     7141 gatgatgatg aagacgatgc acaagatggt accgaggatg tggcagcata ataagcttag
     7201 agactcttat caagaatccc atctcttgct tgcttttttt tgttgtcttc cctttgatag
     7261 ggtttgtttt tcttgtttca gtgactttct atgttaaaag ataatgtcag taaaaggatt
     7321 tggttttcta ttattctgaa tcgattacgg aagattcttg cttaattcca atctatacaa
     7381 gtatcgtgaa ataatgaccg tttatgtgat taggagacgt gtttcattaa taaaatataa
     7441 gatcaataca ttgttagtag tgataaacta tgtacaaatt gtattgattg taaaagaaac
     7501 acaataggtt ccttttttct acaatatatt gtgacagact ctctgtttta acgaatgaat
     7561 taaatttgtc aacgaaaaaa ttaaagaatg atgtgatcac tttgcacctt ggttcatctt
     7621 cagaggcttc tgcatgttcc atgcattgag gctctctacg gtcacaggtt gtgagccgtt
     7681 gttaaagaca aacaaatgcg ctaccacaga atcaggggat aacgcaggaa agaacatgaa
     7741 ggccttgaca ggatatattg gcgggtaaac taagtcgctg tatgtgtttg tttgagatct
     7801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8281 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8341 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8581 atcaatctaa agtactgaga gatcccctca taatttcccc aaagcgtaac catgtgtgaa
     8641 taaattttga gctagtaggg ttgcagccac gagtaagtct tcccttgtta ttgtgtagcc
     8701 agaatgccgc aaaacttcca tgcctaagcg aactgttgag agtacgtttc gatttctgac
     8761 tgtgttagcc tggaagtgct tgtcccaacc ttgtttctga gcatgaacgc ccgcaagcca
     8821 acatgttagt tgaagcatca gggcgattag cagcatgata tcaaaacgct ctgagctgct
     8881 cgttcggcta tggcgtaggc ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc
     8941 ttcaatctgc attcgcttcg aatagatatt aacaagttgt ttgggtgttc gaatttcaac
     9001 aggtaagtta gttgctagaa cccatggctc ctttgccgac gctgagtaga ttttaggtga
     9061 cgggtggtga caatgagtcc gtgtcgagcg ctgatttttt cggcctttag agcgagattt
     9121 atacaataga atttggcatg agattggatt gcttttagtc agcctcttat agcctaaagt
     9181 ctttgagtga ctagatgaca tatcatgtaa gttgctgata ggtttccagt tttccgctcc
     9241 taggtctgca tattgtactt ttcctcttac tcgacttaac cagtaccaac ccagcttctc
     9301 aacggattta taccatggca ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt
     9361 actcggtaga atgctcgcaa ggtcggctag aaattggtca tgagctttct ttgaacattg
     9421 ctctgaaagc gggaacgctt tctcataaag agtaacagaa cgaccgtgta gtgcgactga
     9481 agctcgcaat accataagtc gtttttgctc acgaatatca gaccagtcaa caagtacaat
     9541 gggcatcgta ttgcccgaac agataaagct agcatgccaa cggtatacag cgagtcgctc
     9601 tttgtggagg tgacgattac ctaacaatcg gtcgattcgt ttgatgttat gttttgttct
     9661 cgctttggtt ggcaggttac ggccaagttc ggtaagagtg agagttttac agtcaagtaa
     9721 tgcgtggcaa gccaacgtta agctgttgag tcgttttaag tgtaattcgg ggcagaattg
     9781 gtaaagagag tcgtgtaaaa tatcgagttc gcacatcttg ttgtctgatt attgattttt
     9841 cgcgaaacca tttgatcata tgacaagatg tgtatccacc ttaacttaat gatttttacc
     9901 aaaatcatta ggggattcat cagtctaaag tatatatgtg taacattggt ctagtgatta
     9961 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
    10021 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
    10081 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
    10141 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
    10201 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
    10261 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
    10321 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
    10381 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
    10441 ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc
    10501 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
    10561 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
    10621 ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt
    10681 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
    10741 tgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
    10801 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
    10861 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca
    10921 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
    10981 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga
    11041 tgatggggcg attcaggcga tccccatcca acagcccgcc gtcgagcggg cttttttatc
    11101 cccggaagcc tgtggataga gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc
    11161 cttgattcac ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag
    11221 ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct
    11281 aatcaaaaag gcacgtgaga acgctaatag ccctttcaga tcaacagctt gcaaacaccc
    11341 ctcgctccgg caagtagtta cagcaagtag
//
