LOCUS       sequence-387974-        6473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a human PTPN22 mutant (exon 1-17) with 
            sfCherry(11) tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6473)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfCherry11-Ex1-17-PTPN22
FEATURES             Location/Qualifiers
     source          1..6473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2974..2993)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2975..2993)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3267..3388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3304..3323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3358..3377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3395..3850)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3532..3553)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3744..3763
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3877..3981
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3983..4340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3983..4174
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4006..4026)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4191..4326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4375..5169
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4375..5169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4429..4448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5039..5058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5357..5376)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5401..5448
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5777..6365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6266..6285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat gtacaccatc gtggagcagt acgagagagc cgaggccaga
      721 cacagcaccg gtggcggcat ggaccaaaga gaaattctgc agaagttcct ggatgaggcc
      781 caaagcaaga aaattactaa agaggagttt gccaatgaat ttctgaagct gaaaaggcaa
      841 tctaccaagt acaaggcaga caaaacctat cctacaactg tggctgagaa gcccaagaat
      901 atcaagaaaa acagatataa ggatattttg ccctatgatt atagccgggt agaactatcc
      961 ctgataacct ctgatgagga ttccagctac atcaatgcca acttcattaa gggagtttat
     1021 ggacccaagg cttatattgc cacccagggt cctttatcta caaccctcct ggacttctgg
     1081 aggatgattt gggaatatag tgtccttatc attgttatgg catgcatgga gtatgaaatg
     1141 ggaaagaaaa agtgtgagcg ctactgggct gagccaggag agatgcagct ggaatttggc
     1201 cctttctctg tatcctgtga agctgaaaaa aggaaatctg attatataat caggactcta
     1261 aaagttaagt tcaatagtga aactcgaact atctaccagt ttcattacaa gaattggcca
     1321 gaccatgatg taccttcatc tatagaccct attcttgagc tcatctggga tgtacgttgt
     1381 taccaagagg atgacagtgt tcccatatgc attcactgca gtgctggctg tggaaggact
     1441 ggtgttattt gtgctattga ttatacatgg atgttgctaa aagatgggat aattcctgag
     1501 aacttcagtg ttttcagttt gatccgggaa atgcggacac agaggccttc attagttcaa
     1561 acgcaggaac aatatgaact ggtctacaat gctgtattag aactatttaa gagacagatg
     1621 gatgttatca gagataaaca ttctggaaca gagagtcaag caaagcattg tattcctgag
     1681 aaaaatcaca ctctccaagc agactcttat tctcctaatt taccaaaaag taccacaaaa
     1741 gcagcaaaaa tgatgaacca acaaaggaca aaaatggaaa tcaaagaatc ttcttccttt
     1801 gactttagga cttctgaaat aagtgcaaaa gaagagctag ttttgcaccc tgctaaatca
     1861 agcacttctt ttgactttct ggagctaaat tacagttttg acaaaaatgc tgacacaacc
     1921 atgaaatggc agacaaaggc atttccaata gttggggagc ctcttcagaa gcatcaaagt
     1981 ttggatttgg gctctctttt gtttgaggga tgttctaatt ctaaacctgt aaatgcagca
     2041 ggaagatatt ttaattcaaa ggtgccaata acacggacca aatcaactcc ttttgaattg
     2101 atacagcaga gagaaaccaa ggaggtggac agcaaggaaa acttttctta tttggaatct
     2161 caaccacatg attcttgttt tgtagagatg caggctcaaa aagtaatgca tgtttcttca
     2221 gcagaactga attattcact gccatatgac tctaaacacc aaatacgtaa tgcctctaat
     2281 gtaaagcacc atgactctag tgctcttggt gtatattctt acataccttt agtggaaaat
     2341 ccttattttt catcatggcc tccaagtggt accagttcta agatgtctct tgatttacct
     2401 gagaagcaag atggaactgt ttttccttct tctctgttgc caacatcctc tacatccctc
     2461 ttctcttatt acaattcaca tgattcttta tcactgaatt ctccaaccaa tatttcctca
     2521 ctattgaacc aggagtcagc tgtactagca actgctccaa ggatagatga tgaaatcccc
     2581 cctccacttc ctgtacggac acctgaatca tttattgtgg ttgaggaagc tggagaattc
     2641 tcaccaaatg ttcccaaatc cttatcctca gctgtgaagg taaaaattgg aacatcactg
     2701 gaatggggtg gaacatctga accaaagaaa tttgatgact ctgtgatact tagaccaagc
     2761 aagagtgtaa aactccgaag tcctaaatca gaactacatc aagatcgttc ttctccccca
     2821 cctcctctcc cagaaagaac tctagagtcc ttctttcttg ccgatgaaga ttaataaaac
     2881 tccagattta taataatatg ggctgcaagt acacctcgag ggggggcccg gtaccttaat
     2941 taattaaggt accaggtaag tgtacccaat tcgccctata gtgagtcgta ttacaattca
     3001 ctcgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggagatc caatttttaa
     3061 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt cacagtccca
     3121 aggctcattt caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca
     3181 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3241 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3361 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     3421 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3481 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3541 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3601 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3661 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3721 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3781 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3841 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3961 taatattgaa aaaggaagaa tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4021 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4081 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4141 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4201 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4261 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4321 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4381 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4441 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4501 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4561 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4621 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4681 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4741 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4801 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagaacat
     4861 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4921 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4981 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5041 ttggctaccc gtgatattgc tgaagaactt ggcggcgaat gggctgaccg cttcctcgtg
     5101 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5161 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5221 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5281 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5341 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5401 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5461 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5521 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5581 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5641 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5701 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5761 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5821 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5881 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5941 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6001 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6061 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6421 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcc
//
