LOCUS       sequence-388014-        3648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 mTurq-tUBQ10 for integrase target construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L0-T3 mTurq-tUBQ10
FEATURES             Location/Qualifiers
     source          1..3648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..42
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(58..60,61..63,64..777)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(103..124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(364..383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     711..732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1448..1466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1534..1638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1860..2651
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2822..3410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3311..3330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3564..3581
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggtctcaa cctaaacaat gccaaagaag aagaggaaag tcggaagtgg tagtggtatg
       61 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      121 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      181 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      241 gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
      301 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      361 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      421 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      481 ctggagtaca actacatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
      541 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
      601 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      661 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      721 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa
      781 atctcgtctc tgttatgctt aagaagttca atgtttcgtt tcatgtaaaa ctttggtggt
      841 ttgtgttttg gggccttgta taatccctga tgaataagtg ttctactatg tttccgttcc
      901 tgttatctct ttctttctaa tgacaagtcg aacttcttct ttatcatcgc ttcgttttta
      961 ttatctgtgc ttcttttgtt taatacgcct gcaaagtgac tcgactctgt ttagtgcagt
     1021 tctgcgaaac ttgtaaatag tccaattgtt ggcctctagt aatagatgta gcgaaagtgt
     1081 tgagctgttg ggttctaagg atggcttgaa catgttaatc ttttaggttc tgagtatgat
     1141 gaacattcgt tgttgctaag aaatgcctgt aatgtcccac aaatgtagaa aatggttcgt
     1201 acctttgtcc aagcattgat atgtctgatg agaggaaact gcaagatact gagcttggtt
     1261 taacgaagga gaggcagttt cttccttcca aagcatttca tttgacaatg ccttgatcat
     1321 cttaagtaga gtttctgttg tggaaagttt gaaactttga agaaacgact ctcaagtaaa
     1381 ttgatgatca caagtgaaag tgtatgttcg cttgagacca cgaagtggct cttcagtgga
     1441 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1501 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1561 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1621 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1681 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac
     1741 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1801 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1861 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1921 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1981 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2041 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2101 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2161 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2221 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2281 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2341 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2401 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2461 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2521 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2581 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     2641 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2701 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2761 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2941 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3181 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3241 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3301 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3361 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3421 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3481 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3541 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgctcaa
     3601 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactct
//
