LOCUS       sequence-388015-       19014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pcoCasphi driven by UBQ10 gene 
            promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and 
            flanked by ribozymes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 19014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: 
            pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
FEATURES             Location/Qualifiers
     source          1..19014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..26)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(23..45)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     928..948
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4195..4214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4448..4465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4480..4620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4546..4565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4706..4728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4964..5158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5224..6297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6726..7355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     ncRNA           complement(9584..9651)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(9708..9744)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     intron          16750..16938
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             18257..18277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             18284..18304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             18323..18370
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2821 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2881 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2941 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3001 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3061 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3121 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3181 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3241 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3301 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3361 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3421 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3481 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3541 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3601 actggatgaa ttgttttagt acytakwawg crwrwccmwa akcccttaac gtgagtkttc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4501 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4621 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4681 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4741 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4801 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4861 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4921 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4981 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     5041 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     5101 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     5161 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     5221 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     5281 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     5341 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     5401 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5461 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5521 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5581 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5641 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5701 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5761 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5821 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5881 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5941 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     6001 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     6061 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     6121 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     6181 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     6241 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta gatcactagt
     8941 gttgtcaatc aattggcaag tcataaaatg cattaaaaaa tattttcata ctcaactaca
     9001 aatccatgag tataactata attataaagc aatgattaga atctgacaag gattctggaa
     9061 aattacataa aggaaagttc ataaatgtct aaaacacaag aggacatact tgtattcagt
     9121 aacatttgca gcttttctag gtctgaaaat atatttgttg cctagtgaat aagcataatg
     9181 gtacaactac aagtgtttta ctcctcatat taacttcggt cattagaggc cacgatttga
     9241 cacattttta ctcaaaacaa aatgtttgca tatctcttat aatttcaaat tcaacacaca
     9301 acaaataaga gaaaaaacaa ataatattaa tttgagaatg aacaaaagga ccatatcatt
     9361 cattaactct tctccatcca tttccatttc acagttcgat agcgaaaacc gaataaaaaa
     9421 cacagtaaat tacaagcaca acaaatggta caagaaaaac agttttccca atgccataat
     9481 actcaaactc agtaggattc tggtgtgtgc gcaatgaaac tgatgcattg aacttgacga
     9541 acgttgtcga aaccgatgat acgaacgaaa gctctttaat taagtcccat tcgccatgcc
     9601 gaagcatgtt gcccagccgg cgccagcgag gaggctggga ccatgccggc cattggctgg
     9661 attgtcaact ggtcccctcg tgaggggcaa tcgttgagcg ttccgacgac gagcttactc
     9721 gtttcgtcct cacggactca tcaggtcgga gctgaggcct gttaatcaga aaaactcaga
     9781 ttaatcgaca aattcgatcg cacaaactag aaactaacac cagatctaga tagaaatcac
     9841 aaatcgaaga gtaattattc gacaaaactc aaattatttg aacaaatcgg atgatatcta
     9901 tgaaacccta atcgagaatt aagatgatat ctaacgatca aacccagaaa atcgtcttcg
     9961 atctaagatt aacagaatct aaaccaaaga acatatacga aattgggatc gaacgaaaac
    10021 aaaatcgaag attttgagag aataaggaac acagaaattt accttgatca cggtagagag
    10081 aattgagaga aagtttttaa gattttgaga aattgaaatc tgaattgtga agaagaagag
    10141 ctctttgggt attgttttat agaagaagaa gaagaaaaga cgaggacgac taggtcacga
    10201 gaaagctaag gcggtgaagc aatagctaat aataaaatga cacgtgtatt gagcgttgtt
    10261 tacacgcaaa gttgtttttg gctaattgcc ttatttttag gttgaggaaa agtatttgtg
    10321 ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa ccactttgac gccgtttatt
    10381 actgactcgt cgacaaccac aatttctaac ggtcgtcata agatccagcc gttgagattt
    10441 aacgatcgtt acgatttata tttttttagc attatcgttt tattttttaa atatacggtg
    10501 gagctgaaaa ttggcaataa ttgaaccgtg ggtcccactg cattgaagcg tatttcgtat
    10561 tttctagaat tcttcgtgct ttatttcttt tcctttttgt ttttttttgc catttatcta
    10621 atgcaagtgg gcttataaaa tcagtgaatt tcttggaaaa gtaacttctt tatcgtataa
    10681 catattgtga aattatccat ttcttttaat tttttagtgt tattggatat ttttgtatga
    10741 ttattgattt gcataggata atgacttttg tatcaagttg gtgaacaagt ctcgttaaaa
    10801 aaggcaagtg gtttggtgac tcgatttatt cttgttattt aattcatata tcaatggatc
    10861 ttatttgggg cctggtccat atttaacact cgtgttcagt ccaatgacca ataatatttt
    10921 ttcattaata acaatgtaac aagaatgata cacaaaacat tctttgaata agttcgctat
    10981 gaagaaggga acttatccgg tcctagatca tcagttcata caaacctcca tagagttcaa
    11041 catcttaaac aagaatatcc tgatctgaag aatgtggagg ctttagtccc ttggatactt
    11101 gggaggctgt ggaagaacag aaacgagctg gtgcttaaag ggagggaatt tggaaccaat
    11161 gaggtattag taaggacaca agaagatgca gatgagtgga ttagaaggaa agaggctcag
    11221 aatgtaagga aagcaccaac cacaacaccg tcgggacaga cgagaaacat gggaagctcc
    11281 accacaatct tgggttaagt gcaactttga tggggcatgg ccaacagaag gattaaaatg
    11341 tggcttaggg tgggtgcttc gcgatcatac agggaaggtg ttatggttag gtgcaagagc
    11401 tgtggtaaaa gtaagaagcg tgctggaagt agaagtggag gctcttagat gggctgtgct
    11461 gtcattatcc cgattcaatt ataggaagat catttttgag gtggattctc agcaacttgt
    11521 gtctttggtt acatgaaagt tatgcttgtc aagtctcaat ccaattatcc aagacataaa
    11581 gtatctactt agcaagtttg aggattttat gcttgtgcat acaagccgag aaggaaatgg
    11641 agtggcagat agaatagcta aggaatctct ttcttttgag aattatgatc taaagttgta
    11701 ttctattgta ccaatttggg ttaaaagctc tgttgagcta gactcatatg catgcggccg
    11761 cgttcctaac acctggagaa ccttttatgt acttcacaac cctcaatgct gcttccaggt
    11821 gtgactgttt gggttgctgc atgaattgac tcagcacctg cactgcaaag ctgatatctg
    11881 gccttgtgat tgtcaagtag agaagctttc ccaccagtct ctgataggaa cccacatcct
    11941 ctaactctgc atcatcagtc tttcctaagt gcttgtcata ttctacagta gtcagccttt
    12001 ggttctgttc cattggtgtt gacactggtc tgcagcctcc cagaccaaca cctgatatca
    12061 gttccagtgc atacttcctc tggttcagta ggattccctt ttctgatctc agcacctcaa
    12121 tgcctaagaa gtattttagt tctcccaaat ctttcatctt gaaatgctga tgcagggttg
    12181 cctttgcttc tgaaatcaaa acattgctgc tgcctgttat taacagatca tccacataaa
    12241 tcaggattat gacaaggtca gtcccctctc ttttggtaaa caaggagtga tcataagcac
    12301 tttgcataaa accagcctgc ataaggacag tggtaagctt gatgttccac tgccttgatg
    12361 cttgctttaa accatagagg gattcaacag cctgcacact ttggtctccc cttggctgtg
    12421 aaaaccctga ggcagagaca tataaacttc ttccatgagg tcaccttgta gaaaagcatt
    12481 gttgacatcc atctggaaaa ggaaccagcc cttggaagca gcaacagata tgacagcttt
    12541 tacagtgacc attttggcca ctggagaaaa agtttcatgg tagtcaaggc cttcttgctg
    12601 agtgtatccc ttggccacta gccttgcctt aaacctgtca acttcaccat tagctttgta
    12661 tttaattttg tacacccatt tggaccctat aggctgttta ccagggggta aagggacaat
    12721 ctcccaggtg ttattatcct caagagcctg tatctcaagg gacatggcct ccatccattt
    12781 ctcatcttga gctgcttctt tgaaggattt aggttcagta taagtggaga aagcactcaa
    12841 ataagcttga tagtgaacag gcaagtgatc ataggaaaca tagttggcta tagggtatgg
    12901 aacatcccta gagcctttgt tcagtgtcac aaagtccttg agccagatgg gaggacctgc
    12961 attgcgttta ggtctagtgt gcaggtttgc tggaactaga gagggatcag ctacagcagt
    13021 atggtgctca aattcagcat ttgctaggtc aggctcagct gactcaactg actctgcagg
    13081 agcatgcagg tggcctgaag gtgcagcatc agctgaagtg atatgatttt gggtagaatg
    13141 agtccccaaa gtgatatcct cattggcctc tacaatgtca ggaataagaa ctggagtatc
    13201 atcatcatca gtatgaaagc tggaaggaaa aatatcatta tacactagct gcaaagctgt
    13261 atcttcagag ctaagtgaac ctgctcgagt gaacatatca ggttcatggg agatggactc
    13321 tttgaaaggg aactgaaact ctctgaaaac tacatccctg ctcactatga tcaccttatt
    13381 atccaagtca tacaacctgt aacccttttg agtctcagaa taaccaatga agatggttcg
    13441 cttggctcta ggtgcaagtt tgtcaccttt gggcagtgtt gctgcaaaag caagacaccc
    13501 aaacactctc aagtgatcaa gcttggcatg tttctgatag agtagttcat atggacattt
    13561 gccttgtaag attggagtag ggagtctatt gatcaggtat acaacagtct tgacacagtc
    13621 tccccaaaac ctggtaggta caccactctg aaacttaagt gcccttgcca tctcaaggat
    13681 gtgtctgtgc tttctctcca caacaccatt ctgttgtggt gtgtagggac agctactttg
    13741 atgaacaatc ccaagagagg ccagcaactc attacaactt ctagtatcct aggaaggtac
    13801 cagtctagct caacagagct tttaacccaa attggtacaa tagaatacaa ctttagatca
    13861 taattctcaa aagaaagaga ttccttagct attctatctg ccactccatt tccttctcgg
    13921 cttgtatgca caagcataaa atcctcaaac ttgctaagta gatactttat gtcttggata
    13981 attggattga gacttgacaa gcataacttt catgtaacca aagacacaag ttgctgagaa
    14041 tccacctcaa aaatgatctt cctataattg aatcgggata atgacagcac agcccatcta
    14101 agagcctcca cttctacttc cagcacgctt cttactttta ccacagctct tgcacctaac
    14161 cataacacct tccctgtatg atcgcgaagc acccacccta agccacattt taatccttct
    14221 gttggccatg ccccatcaaa gttgcactta acccaagatt gtggtggagc ttcccatgtt
    14281 tctcgtctgt cccgacggtg ttgtggttgg tgctttcctt acattctgag cctctttcct
    14341 tctaatccac tcatctgcat cttcttgtgt ccttactaat acctcattgg ttccaaattc
    14401 cctcccttta agcaccagct cgtttctgtt cttccacagc ctcccaagta tccaagggac
    14461 taaagcctcc acattcttca gatcaggata ttcttgttta agatgttgaa ctctatggag
    14521 gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc gaacttattc
    14581 aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat attattggtc
    14641 attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc attgatatat
    14701 gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta acgagacttg
    14761 ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata caaaaatatc
    14821 caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata cgataaagaa
    14881 gttacttttc caagaaattc actgatttta taagcccact tgcattagat aaatggcaaa
    14941 aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg aaatacgctt
    15001 caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg tatatttaaa
    15061 aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc tcaacggctg
    15121 gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa
    15181 agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc
    15241 tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac
    15301 acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt
    15361 cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca
    15421 attcagattt caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc
    15481 aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc
    15541 caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaag acgattttct
    15601 gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat
    15661 ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag
    15721 atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga
    15781 ttaacagatg ccgaagcccg ccgtcgaatc agagttttcc aaagtcctca agaaacactt
    15841 tcctggggag cgttttaggt ctagctatat gaagaggggg ggtaaaattc tggcagcaca
    15901 aggcgaggaa gctgtagtgg cgtacttgca gggaaagagt gaggaggaac cgccgaattt
    15961 tcagccgccg gcgaagtgcc acgtggtcac caaaagcagg gatttcgcag aatggcccat
    16021 aatgaaagcc tctgaagcca tacagaggta catctatgcg ctcagcacta cagagcgagc
    16081 tgcctgcaaa ccgggtaaga gctcagaaag tcacgcggcc tggttcgcgg ctacaggggt
    16141 gagcaatcac ggctattctc atgtacaagg tcttaacctg atctttgacc acacgctagg
    16201 acgatacgat ggcgttttaa agaaagtaca gcttcgaaat gagaaggccc gagctagatt
    16261 ggaaagcata aacgcctcac gagctgatga aggattgccg gaaatcaagg ccgaggagga
    16321 ggaggtagct acaaatgaaa caggtcatct actacagccg ccaggcataa acccatcatt
    16381 ctacgtctac cagaccatat ctccgcaggc ttaccgacca agggacgaaa tagtgttacc
    16441 acccgagtac gccggttacg tcagggatcc gaacgctccg attccactgg gcgtggtcag
    16501 gaaccgttgt gacatacaga agggttgccc gggatatata cccgagtggc agagggaagc
    16561 tggtacggca attagtccca agacaggaaa agcagtgacg gttccaggac ttagcccgaa
    16621 gaagaataaa cgtatgcgta ggtactggag gtcagaaaag gagaaggctc aagatgcact
    16681 tctcgtaact gtaaggatag gtaccgattg ggtagtcata gacgtaagag gattactaag
    16741 aaacgccagg taagtttctg cttctacctt tgatatatat ataataatta tcattaatta
    16801 gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt
    16861 gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca
    16921 aaatttgttg atgtgcaggt ggaggactat tgctcccaag gacataagtt taaatgcact
    16981 tctagattta tttaccggtg atccagtcat cgatgtcaga cgaaacatcg tgaccttcac
    17041 ctataccttg gacgcttgcg gaacttatgc tagaaaatgg actctcaagg gaaaacagac
    17101 aaaagcaacc ttagataaac tgacagcgac acaaactgtg gccttagttg ctatagatct
    17161 gggacaaaca aacccaatta gcgcgggtat cagtcgtgtc acacaggaga acggggccct
    17221 ccagtgcgaa ccgcttgatc gttttacatt gcctgatgac cttttgaaag atatttctgc
    17281 gtaccgaatt gcatgggacc gtaacgagga ggaactcagg gccagatccg ttgaggcact
    17341 cccagaggca caacaagcag aggtcagagc tctggacggg gtctccaaag agaccgcgcg
    17401 tacacagttg tgcgcggact tcggtctgga cccaaagcga ctaccgtggg ataaaatgag
    17461 tagcaatacc acgttcataa gcgaggcgct cctttccaac agcgtatccc gtgaccaagt
    17521 attctttacc ccggccccaa agaaaggagc caagaagaaa gcaccggtgg aagtgatgcg
    17581 aaaagacagg acatgggcgc gagcgtacaa accacgactc tcagtagaag cacaaaagtt
    17641 gaaaaatgag gctctttggg ctttgaagcg tacctctcca gaatatctaa agttgtcacg
    17701 acgtaaagaa gaattgtgta ggaggtccat taattacgtg atagaaaaaa ctagaaggcg
    17761 aacccaatgc caaattgtga tccccgttat agaagatcta aatgtccgat ttttccacgg
    17821 gtctggcaaa cgactcccgg gctgggataa cttcttcacg gcaaagaagg aaaaccgatg
    17881 gtttatccaa gggctacata aggcgttttc tgacttaagg acccaccgat ccttctacgt
    17941 gttcgaggtg cgtccagaga gaacatcaat cacctgtccg aaatgcgggc actgtgaagt
    18001 ggggaacagg gatggagaag cctttcagtg tctcagttgc ggtaagacat gcaacgcaga
    18061 tctggacgta gcgacacata acttgactca agtggcgctc accggcaaaa caatgccgaa
    18121 gagagaggag cctagagatg cacaagggac agcccccgcg agaaagacga agaaagctag
    18181 caagtcaaag gcacccccgg ctgaacgtga ggatcagact cccgctcaag agccgtccca
    18241 aacgtccgga tccggaccga agaaaaagcg aaaggtagag gatcctaaaa agaagcgtaa
    18301 agtctccttg ggttctggct ccgactataa ggatgacgat gacaaagact ataaggatga
    18361 cgacgacaag tgaagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
    18421 atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
    18481 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
    18541 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
    18601 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
    18661 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
    18721 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
    18781 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
    18841 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
    18901 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
    18961 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacga attc
//
