LOCUS       sequence-388016-        2543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden
            gate linkers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L0-I18 Ub-Arg GG1-GG2
FEATURES             Location/Qualifiers
     source          1..2543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..289
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(394..412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        480..584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             806..1597
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1768..2356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2257..2276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2510..2527
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca
       61 aatgcaaatc ttcgttaaaa ctctgacggg caaaacgatc acgttagaag tagagccatc
      121 cgatacgata gaaaacgtga aggctaaaat tcaggacaaa gagggaatac cgcctgacca
      181 acagaggctc atttttgcag gcaagcagct tgaagatggt cgaacactga gtgattacaa
      241 catccaaaag gagagcactc tgcatctagt cttacgtctg cgagggggcc gtcatggtag
      301 cggtgcgtgg ttgctgcccg tgagtctcgt gaagaggaag acgactcttg ctggagcctg
      361 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
      421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
      601 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      661 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      721 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      781 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
      841 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      901 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
      961 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     1021 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     1081 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1141 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1201 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1261 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1321 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1381 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1441 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1501 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1561 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1621 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1681 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1741 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2521 gagcgaggaa gcggaagagc gcc
//
