LOCUS       sequence-388023-       18960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene 
            promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter,
            flanked by ribozymes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18960)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 18960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: 
            pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
FEATURES             Location/Qualifiers
     source          1..18960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(645..692)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             complement(711..731)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(738..758)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2077..2265
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     ncRNA           9217..9253
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           9310..9377
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(10062..10079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10062..10078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10071..10093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10281..10305
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11606..12235
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12664..13737
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13803..13997
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14233..14255)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14341..14481
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14396..14415
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14496..14513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14667..15255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14747..14766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15342..16136)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16561..16585
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    16663..16837
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(16878..17903)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(17970..18646)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(18013..18033)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    18837..18858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18873..18878,18879..18896,18897..18903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18911..18927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18916..18938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18935..18951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18935..18951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcgttg tcaatcaatt ggcaagtcat aaaatgcatt aaaaaatatt ttcatactca
       61 actacaaatc catgagtata actataatta taaagcaatg attagaatct gacaaggatt
      121 ctggaaaatt acataaagga aagttcataa atgtctaaaa cacaagagga catacttgta
      181 ttcagtaaca tttgcagctt ttctaggtct gaaaatatat ttgttgccta gtgaataagc
      241 ataatggtac aactacaagt gttttactcc tcatattaac ttcggtcatt agaggccacg
      301 atttgacaca tttttactca aaacaaaatg tttgcatatc tcttataatt tcaaattcaa
      361 cacacaacaa ataagagaaa aaacaaataa tattaatttg agaatgaaca aaaggaccat
      421 atcattcatt aactcttctc catccatttc catttcacag ttcgatagcg aaaaccgaat
      481 aaaaaacaca gtaaattaca agcacaacaa atggtacaag aaaaacagtt ttcccaatgc
      541 cataatactc aaactcagta ggattctggt gtgtgcgcaa tgaaactgat gcattgaact
      601 tgacgaacgt tgtcgaaacc gatgatacga acgaaagctc ttcacttgtc gtcgtcatcc
      661 ttatagtctt tgtcatcgtc atccttatag tcggagccag aacccaagga gactttacgc
      721 ttctttttag gatcctctac ctttcgcttt ttcttcggtc cggatccgga cgtttgggac
      781 ggctcttgag cgggagtctg atcctcacgt tcagccgggg gtgcctttga cttgctagct
      841 ttcttcgtct ttctcgcggg ggctgtccct tgtgcatctc taggctcctc tctcttcggc
      901 attgttttgc cggtgagcgc cacttgagtc aagttatgtg tcgctacgtc cagatctgcg
      961 ttgcatgtct taccgcaact gagacactga aaggcttctc catccctgtt ccccacttca
     1021 cagtgcccgc atttcggaca ggtgattgat gttctctctg gacgcacctc gaacacgtag
     1081 aaggatcggt gggtccttaa gtcagaaaac gccttatgta gcccttggat aaaccatcgg
     1141 ttttccttct ttgccgtgaa gaagttatcc cagcccggga gtcgtttgcc agacccgtgg
     1201 aaaaatcgga catttagatc ttctataacg gggatcacaa tttggcattg ggttcgcctt
     1261 ctagtttttt ctatcacgta attaatggac ctcctacaca attcttcttt acgtcgtgac
     1321 aactttagat attctggaga ggtacgcttc aaagcccaaa gagcctcatt tttcaacttt
     1381 tgtgcttcta ctgagagtcg tggtttgtac gctcgcgccc atgtcctgtc ttttcgcatc
     1441 acttccaccg gtgctttctt cttggctcct ttctttgggg ccggggtaaa gaatacttgg
     1501 tcacgggata cgctgttgga aaggagcgcc tcgcttatga acgtggtatt gctactcatt
     1561 ttatcccacg gtagtcgctt tgggtccaga ccgaagtccg cgcacaactg tgtacgcgcg
     1621 gtctctttgg agaccccgtc cagagctctg acctctgctt gttgtgcctc tgggagtgcc
     1681 tcaacggatc tggccctgag ttcctcctcg ttacggtccc atgcaattcg gtacgcagaa
     1741 atatctttca aaaggtcatc aggcaatgta aaacgatcaa gcggttcgca ctggagggcc
     1801 ccgttctcct gtgtgacacg actgataccc gcgctaattg ggtttgtttg tcccagatct
     1861 atagcaacta aggccacagt ttgtgtcgct gtcagtttat ctaaggttgc ttttgtctgt
     1921 tttcccttga gagtccattt tctagcataa gttccgcaag cgtccaaggt ataggtgaag
     1981 gtcacgatgt ttcgtctgac atcgatgact ggatcaccgg taaataaatc tagaagtgca
     2041 tttaaactta tgtccttggg agcaatagtc ctccacctgc acatcaacaa attttggtca
     2101 tatattagaa aagttataaa ttaaaatata cacacttata aactacagaa aagcaattgc
     2161 tatatactac attcttttat tttgaaaaaa atatttgaaa tattatatta ctactaatta
     2221 atgataatta ttatatatat atcaaaggta gaagcagaaa cttacctggc gtttcttagt
     2281 aatcctctta cgtctatgac tacccaatcg gtacctatcc ttacagttac gagaagtgca
     2341 tcttgagcct tctccttttc tgacctccag tacctacgca tacgtttatt cttcttcggg
     2401 ctaagtcctg gaaccgtcac tgcttttcct gtcttgggac taattgccgt accagcttcc
     2461 ctctgccact cgggtatata tcccgggcaa cccttctgta tgtcacaacg gttcctgacc
     2521 acgcccagtg gaatcggagc gttcggatcc ctgacgtaac cggcgtactc gggtggtaac
     2581 actatttcgt cccttggtcg gtaagcctgc ggagatatgg tctggtagac gtagaatgat
     2641 gggtttatgc ctggcggctg tagtagatga cctgtttcat ttgtagctac ctcctcctca
     2701 cctgaagatc cggccttctc atttcgaagc tgtactttct ttaaaacgcc atcgtatcgt
     2761 cctagcgtgt ggtcaaagat caggttaaga ccttgtacat gagaatagcc gtgattgctc
     2821 acccctgtag ccgcgaacca ggccgcgtga ctttctgagc tcttacccgg tttgcaggca
     2881 gctcgctctg tagtgctgag cgcatagatg tacctctgta tggcttcaga ggctttcatt
     2941 atgggccatt ctgcgaaatc cctgcttttg gtgaccacgt ggcacttcgc cggcggctga
     3001 aaattcggcg gttcctcctc actctttccc tgcaagtacg ccactacagc ttcctcgcct
     3061 tgtgctgcca gaattttacc ccccctcttc atatagctag acctaaaacg ctccccagga
     3121 aagtgtttct tgaggacttt ggaaaactct gattcgacgg cgggcttcgg catctgttaa
     3181 tcagaaaaac tcagattaat cgacaaattc gatcgcacaa actagaaact aacaccagat
     3241 ctagatagaa atcacaaatc gaagagtaat tattcgacaa aactcaaatt atttgaacaa
     3301 atcggatgat atctatgaaa ccctaatcga gaattaagat gatatctaac gatcaaaccc
     3361 agaaaatcgt cttcgatcta agattaacag aatctaaacc aaagaacata tacgaaattg
     3421 ggatcgaacg aaaacaaaat cgaagatttt gagagaataa ggaacacaga aatttacctt
     3481 gatcacggta gagagaattg agagaaagtt tttaagattt tgagaaattg aaatctgaat
     3541 tgtgaagaag aagagctctt tgggtattgt tttatagaag aagaagaaga aaagacgagg
     3601 acgactaggt cacgagaaag ctaaggcggt gaagcaatag ctaataataa aatgacacgt
     3661 gtattgagcg ttgtttacac gcaaagttgt ttttggctaa ttgccttatt tttaggttga
     3721 ggaaaagtat ttgtgctttg agttgataaa cacgactcgt gtgtgccggc tgcaaccact
     3781 ttgacgccgt ttattactga ctcgtcgaca accacaattt ctaacggtcg tcataagatc
     3841 cagccgttga gatttaacga tcgttacgat ttatattttt ttagcattat cgttttattt
     3901 tttaaatata cggtggagct gaaaattggc aataattgaa ccgtgggtcc cactgcattg
     3961 aagcgtattt cgtattttct agaattcttc gtgctttatt tcttttcctt tttgtttttt
     4021 tttgccattt atctaatgca agtgggctta taaaatcagt gaatttcttg gaaaagtaac
     4081 ttctttatcg tataacatat tgtgaaatta tccatttctt ttaatttttt agtgttattg
     4141 gatatttttg tatgattatt gatttgcata ggataatgac ttttgtatca agttggtgaa
     4201 caagtctcgt taaaaaaggc aagtggtttg gtgactcgat ttattcttgt tatttaattc
     4261 atatatcaat ggatcttatt tggggcctgg tccatattta acactcgtgt tcagtccaat
     4321 gaccaataat attttttcat taataacaat gtaacaagaa tgatacacaa aacattcttt
     4381 gaataagttc gctatgaaga agggaactta tccggtccta gatcatcagt tcatacaaac
     4441 ctccatagag ttcaacatct taaacaagaa tatcctgatc tgaagaatgt ggaggcttta
     4501 gtcccttgga tacttgggag gctgtggaag aacagaaacg agctggtgct taaagggagg
     4561 gaatttggaa ccaatgaggt attagtaagg acacaagaag atgcagatga gtggattaga
     4621 aggaaagagg ctcagaatgt aaggaaagca ccaaccacaa caccgtcggg acagacgaga
     4681 aacatgggaa gctccaccac aatcttgggt taagtgcaac tttgatgggg catggccaac
     4741 agaaggatta aaatgtggct tagggtgggt gcttcgcgat catacaggga aggtgttatg
     4801 gttaggtgca agagctgtgg taaaagtaag aagcgtgctg gaagtagaag tggaggctct
     4861 tagatgggct gtgctgtcat tatcccgatt caattatagg aagatcattt ttgaggtgga
     4921 ttctcagcaa cttgtgtctt tggttacatg aaagttatgc ttgtcaagtc tcaatccaat
     4981 tatccaagac ataaagtatc tacttagcaa gtttgaggat tttatgcttg tgcatacaag
     5041 ccgagaagga aatggagtgg cagatagaat agctaaggaa tctctttctt ttgagaatta
     5101 tgatctaaag ttgtattcta ttgtaccaat ttgggttaaa agctctgttg agctagactg
     5161 gtaccttcct aggatactag aagttgtaat gagttgctgg cctctcttgg gattgttcat
     5221 caaagtagct gtccctacac accacaacag aatggtgttg tggagagaaa gcacagacac
     5281 atccttgaga tggcaagggc acttaagttt cagagtggtg tacctaccag gttttgggga
     5341 gactgtgtca agactgttgt atacctgatc aatagactcc ctactccaat cttacaaggc
     5401 aaatgtccat atgaactact ctatcagaaa catgccaagc ttgatcactt gagagtgttt
     5461 gggtgtcttg cttttgcagc aacactgccc aaaggtgaca aacttgcacc tagagccaag
     5521 cgaaccatct tcattggtta ttctgagact caaaagggtt acaggttgta tgacttggat
     5581 aataaggtga tcatagtgag cagggatgta gttttcagag agtttcagtt ccctttcaaa
     5641 gagtccatct cccatgaacc tgatatgttc actcgagcag gttcacttag ctctgaagat
     5701 acagctttgc agctagtgta taatgatatt tttccttcca gctttcatac tgatgatgat
     5761 gatactccag ttcttattcc tgacattgta gaggccaatg aggatatcac tttggggact
     5821 cattctaccc aaaatcatat cacttcagct gatgctgcac cttcaggcca cctgcatgct
     5881 cctgcagagt cagttgagtc agctgagcct gacctagcaa atgctgaatt tgagcaccat
     5941 actgctgtag ctgatccctc tctagttcca gcaaacctgc acactagacc taaacgcaat
     6001 gcaggtcctc ccatctggct caaggacttt gtgacactga acaaaggctc tagggatgtt
     6061 ccatacccta tagccaacta tgtttcctat gatcacttgc ctgttcacta tcaagcttat
     6121 ttgagtgctt tctccactta tactgaacct aaatccttca aagaagcagc tcaagatgag
     6181 aaatggatgg aggccatgtc ccttgagata caggctcttg aggataataa cacctgggag
     6241 attgtccctt taccccctgg taaacagcct atagggtcca aatgggtgta caaaattaaa
     6301 tacaaagcta atggtgaagt tgacaggttt aaggcaaggc tagtggccaa gggatacact
     6361 cagcaagaag gccttgacta ccatgaaact ttttctccag tggccaaaat ggtcactgta
     6421 aaagctgtca tatctgttgc tgcttccaag ggctggttcc ttttccagat ggatgtcaac
     6481 aatgcttttc tacaaggtga cctcatggaa gaagtttata tgtctctgcc tcagggtttt
     6541 cacagccaag gggagaccaa agtgtgcagg ctgttgaatc cctctatggt ttaaagcaag
     6601 catcaaggca gtggaacatc aagcttacca ctgtccttat gcaggctggt tttatgcaaa
     6661 gtgcttatga tcactccttg tttaccaaaa gagaggggac tgaccttgtc ataatcctga
     6721 tttatgtgga tgatctgtta ataacaggca gcagcaatgt tttgatttca gaagcaaagg
     6781 caaccctgca tcagcatttc aagatgaaag atttgggaga actaaaatac ttcttaggca
     6841 ttgaggtgct gagatcagaa aagggaatcc tactgaacca gaggaagtat gcactggaac
     6901 tgatatcagg tgttggtctg ggaggctgca gaccagtgtc aacaccaatg gaacagaacc
     6961 aaaggctgac tactgtagaa tatgacaagc acttaggaaa gactgatgat gcagagttag
     7021 aggatgtggg ttcctatcag agactggtgg gaaagcttct ctacttgaca atcacaaggc
     7081 cagatatcag ctttgcagtg caggtgctga gtcaattcat gcagcaaccc aaacagtcac
     7141 acctggaagc agcattgagg gttgtgaagt acataaaagg ttctccaggt gttaggaacg
     7201 cggccgcatg catatgagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa
     7261 tacaacttta gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact
     7321 ccatttcctt ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac
     7381 tttatgtctt ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac
     7441 acaagttgct gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac
     7501 agcacagccc atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca
     7561 gctcttgcac ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca
     7621 cattttaatc cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt
     7681 ggagcttccc atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt
     7741 ctgagcctct ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc
     7801 attggttcca aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc
     7861 aagtatccaa gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg
     7921 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     7981 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     8041 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     8101 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     8161 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     8221 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     8281 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     8341 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     8401 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     8461 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     8521 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     8581 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     8641 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     8701 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     8761 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     8821 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     8881 ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt
     8941 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
     9001 cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
     9061 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt
     9121 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa
     9181 tctgagtttt tctgattaac aggcctcagc tccgacctga tgagtccgtg aggacgaaac
     9241 gagtaagctc gtcgtcggaa cgctcaacga ttgcccctca cgaggggacc agttgacaat
     9301 ccagccaatg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacatgcttc
     9361 ggcatggcga atgggactta attaaagagc tttcgttcgt atcatcggtt tcgacaacgt
     9421 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     9481 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     9541 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     9601 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     9661 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     9721 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     9781 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     9841 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     9901 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     9961 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
    10021 actagtgatc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
    10081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    10141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10201 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
    10261 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
    10321 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
    10381 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
    10441 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
    10501 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
    10561 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
    10621 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
    10681 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
    10741 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
    10801 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
    10861 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
    10921 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
    10981 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
    11041 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
    11101 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
    11161 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
    11221 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
    11281 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
    11341 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
    11401 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
    11461 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
    11521 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
    11581 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
    11641 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
    11701 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
    11761 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
    11821 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
    11881 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
    11941 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
    12001 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
    12061 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
    12121 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
    12181 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
    12241 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
    12301 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
    12361 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
    12421 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
    12481 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
    12541 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
    12601 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
    12661 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
    12721 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
    12781 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
    12841 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
    12901 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
    12961 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
    13021 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
    13081 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
    13141 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
    13201 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
    13261 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
    13321 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
    13381 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
    13441 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
    13501 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
    13561 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
    13621 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
    13681 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
    13741 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
    13801 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
    13861 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
    13921 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
    13981 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
    14041 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
    14101 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
    14161 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
    14221 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    14281 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    14341 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
    14401 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    14461 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    14521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    14581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    14641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    14701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    14761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    14821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    14881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    14941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    15001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    15061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    15121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    15181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    15241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    15301 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
    15361 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    15421 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    15481 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    15541 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    15601 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    15661 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    15721 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    15781 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    15841 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    15901 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    15961 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    16021 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    16081 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    16141 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    16201 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    16261 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    16321 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    16381 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    16441 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    16501 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    16561 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    16621 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
    16681 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
    16741 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
    16801 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
    16861 cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
    16921 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
    16981 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
    17041 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
    17101 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
    17161 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
    17221 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
    17281 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
    17341 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
    17401 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
    17461 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
    17521 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
    17581 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
    17641 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
    17701 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
    17761 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
    17821 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
    17881 gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga
    17941 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
    18001 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
    18061 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    18121 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
    18181 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
    18241 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
    18301 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
    18361 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
    18421 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
    18481 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
    18541 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
    18601 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
    18661 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    18721 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    18781 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    18841 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    18901 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
//
