LOCUS       sequence-388058-        6894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the protein of wNeonGreen in neurons of C elegan.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pSF11-wmNeonGreen
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4464..4486)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4586..4605
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4687..4705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4773..4877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4878..4946,4947..5738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5096..5115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5909..6497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6398..6417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6651..6668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6785..6806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6821..6826,6827..6844,6845..6851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6859..6875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6864..6886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6883..6894,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6883..6894,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa artgaggggg cycmcygccc cccccccccy cccccccccc tcttctcctt
      241 gaagctcatc cagacgtcgc tgtcctcctc ctcctcgtcc ccggggcgct gtgtgtgtgt
      301 gtgtgtgctc cgtgagcatg tagcagcttc agcccattta aaggcgcaca ctctctgcat
      361 ctctgcgtgt ctcgacactc gttttttctc ttcttttctg ctgcacacac acacacacac
      421 acacacacgc tcatgccaga gcacttcttg ctccgggggg ctccgcgcgg agcccgagag
      481 gacctcctcc tcctttttct tcttcgcttt ttagttcttc ttgcctcggt ctcgcccctt
      541 gttttgctct ccatgtagta tatacatctg ggtgtgtgtg tgtgtgtgca cctttggagc
      601 attttttgaa cctttccaga actttggagc aacattttca gcttgttcag tgcacttttt
      661 aggctgaaaa tcctattttt aggttttcga aagttttttt ggagcatttt tcgtgcaatt
      721 taggtgcaaa attaaaaaaa agggttttgt agtgaattaa aatttttttg aaactttcgc
      781 ctttttaaaa aaatgtttct aatatcttta aaaattggaa tattttggaa aacaatttgt
      841 tttcaattgg caatttatta attctgagct tctgagcgta aatttttttt tattttggag
      901 caattctgag atgtcttagt gcatttttcg ggcatacata aaaaaaaatt tatcttaata
      961 ataaaaacct taaatttagg cttcaccgtc tggaaattca attttttcag ctgaaaaact
     1021 ttcaaaaaaa taaaaattaa tttttcacat tttttttctc tgaatttttt gagaaatttc
     1081 aaagttattt tattactttt ttagtaatca tatatttatt ctttttccta aaattttaat
     1141 attttatgtt taaaggcttc accgtctgaa aatgcaactt ttttcagatg aaatattaaa
     1201 aaaaaaacct cttttattaa ttttgtgctc caaactttcc cacggtacgc aaatcatgta
     1261 atatgtactt ttaaacatta tttttccaat caaaaatatc caacarcttt tccttacaat
     1321 ttgaaaattt ttattaaaat ttcaaaaaaa tttttttttt ttttaatttc gaattttttt
     1381 aaacttgttg taactttttt ccctgtcggc gccaattaac aaaatacgta gaattttgcg
     1441 cttattgttt tgttagttaa caaatttttg acaactttct tatcagctga gatttaggtg
     1501 tttcagttcg caatctgcgt ctctccttgc tctcctctat tttaaaaccc ccataacttt
     1561 gttcttgtga gagttatcaa aaaattgtca actaacaaaa tatagagcac aaaaatatgc
     1621 acattttgta tgttgacaat tttttgatac cttttttagt tttcgagata tcttagtttt
     1681 agtttagctg tacctttttc tggcaaaaaa cttccaaatt tcggcaattt ttcggcaaat
     1741 ttcccggcaa atttcgccaa gtacatcggg ctctcagaca agtggtctgt gagtggaagt
     1801 cgataaaagt agtttacgtg ctctccgtcc ccccgcttct tctccgtctc tgcttctatt
     1861 tctccattga ctagtcccac tactttttac ggggcggagg gctctcttgc actccagctt
     1921 cttctctttt tgtgctcttt tttgcccgta tgtgggtgct catttatttt tctttggttt
     1981 taaaataaat tcggtttatt aggctactcg taaaaatgaa gaaaaaactt caaattcaaa
     2041 cgacattaaa aaaaaattta ataaaaattt tttgaattgc tcaaaagccc cacctatata
     2101 tatatatagc tccgcctact tctcgcacag accccgccca ctttttgact ccgccccctt
     2161 attggcaaaa gttcttgaaa aaaataaaaa taaaaattca aattgaaaca attttttttt
     2221 aatgaagtag tttttttcta aattgatgaa aatttctaaa cagtttacaa gcaccgccca
     2281 ctttttgact ccgccccttt gttggaaaaa ttcttgtaaa aaaaaataaa aaataaaagc
     2341 ttctctttca aacgattttt agttggtctt tgtatgaaaa gtttttttcc taaaatagaa
     2401 atgtctgaat aatttgcaag ctccgccctt ttcttaccca gactccgccc ctttattgac
     2461 taattttttt ttgtccattt acctgaagtt caaaaaaaaa atttacagtt tcatagagta
     2521 ataatttttc aaaaatctct ttttccaatg tttaatgtgt cattataaac ccgcccagtt
     2581 ttaagctccg cccatttagg ccccgcccat ttatgaccca aatatttttt tctctattca
     2641 cccgtggatt tccctccaac aagttacccc aaaaataaaa ataaaaattc ctgcaggcgc
     2701 ccaccgggat ccggcgcgcc tctagaggat ccccgggatt ggccaaagga cccaaaggta
     2761 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctagcgtcg
     2821 acggtaccaa aaatggtttc aaaaggagag gaagataaca tggctagtct tccagctacc
     2881 catgagcttc acatattcgg aagcatcaat ggagttgact tcgacatggt tggacaggga
     2941 acgggaaatc caaatgacgg atacgaggag ctcaatctta aatccacgaa aggagatctc
     3001 caattctccc catggatact cgttccacac attggatacg gattccacca gtacctccca
     3061 tacccagacg gaatgagccc attccaagct gccatggtcg acggaagcgg ataccaagtc
     3121 caccgcacga tgcaattcga ggatggagct agcctcaccg ttaactatcg ctacacgtac
     3181 gaaggaagcc acatcaaggt aagtttaaac atatatatac taactaaccc tgattattta
     3241 aattttcagg gagaggccca agttaaggta agtttaaaca gttcggtact aactaaccat
     3301 acatatttaa attttcaggg aactggattc ccagccgacg gaccagtcat gacgaacagc
     3361 cttacggctg ctgactggtg ccgctctaaa aaaacttatc caaacgataa aactatcatc
     3421 tctacgttca agtggagcta cacgacggga aacggaaaac gctaccgctc cacggcccgc
     3481 acgacttata ctttcgctaa gccaatggcc gctaattatc ttaaaaacca gccaatgtac
     3541 gtcttccgca aaaccgaact caagcactct aaaacggaac ttaacttcaa agagtggcag
     3601 aaggccttca ccgacgtcat gggaatggat gaactttata aataagagct ccgcatcggc
     3661 cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     3721 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     3781 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     3841 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     3901 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     3961 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
     4021 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
     4081 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
     4141 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
     4201 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
     4261 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt
     4321 tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt
     4381 cccaatatac caaacataac tgtttctact agtcggccgt acgggccctt tcgtctcgcg
     4441 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4501 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4561 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4621 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     4681 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5101 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6721 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6781 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6841 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agct
//
