LOCUS       sequence-388062-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  simplyfied amino acid sequence by alanine scanning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Kuroda Y, Kim PS
  TITLE     Folding of bovine pancreatic trypsin inhibitor (BPTI) variants in 
            which almost half the residues are alanine.
  JOURNAL   J Mol Biol. 2000 May 5;298(3):493-501. doi: 10.1006/jmbi.2000.3622.
  PUBMED    10772865
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/jmbi.2000";
            journalName: "J Mol Biol"; date: "2000-05-5- 5"; volume: "298"; 
            issue: "3"; pages: "493-501"
COMMENT     Sequence Label: BPTI-21
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..83
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(674..692)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      688..735
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(981..998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaattcc cttctagaat aattttgttt aactttaaga aggagatata catatgcatc
       61 accatcacca tcaccatcac cataaagcaa ttttcgtact gaaaggttca ctggacagag
      121 atctcgacag ccgtattgaa ctggaactgc gtaccgatca taaagagctg tctgaacatc
      181 tgctgctggt tgatctcgcc cgtaatgatc tggcacgcat tgctaccccc ggcagccgct
      241 acgtcgccga tctcaccaaa gttgaccgtt attcctatgt gctgcacctc gtctctcgcg
      301 tagtcggcga actgcgtcac gatcttgacg ccctgcacgc ttatcgcgcc gctctgaatc
      361 tggggacgtt aagcggtgcg ccgaaagtac gcgctaagct ttggatgcgc ccggcattct
      421 gcctggaacc accgtacgca ggtccggcga aagcgcgcat catccgttac ttctacaacg
      481 ctgccgcagg cgccgctcag gccttcgtgt acggtggtgc acgtgctaaa cgtaacaact
      541 tcgcctccgc ggcagatgca cttgctgcgt gcgcagctgc ttgaggatcc ccgggtaccg
      601 agctcgaatt catcgatgat atcagatccg gctgctaaca aagcccgaaa ggaagctgag
      661 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      721 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
      781 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
      841 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
      901 tagcgctaga gtcgactgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      961 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcgggtcg ttcggctgcg
     1021 gcgagcggta tcagctcact caagggcggg taaatacggg ttatccacag atcaggggat
     1081 acgcagaaga ccatgtgagc aaaaagggac ccaagccaaa aaagaggccc caggg
//
