LOCUS       sequence-388066-       10023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 
            activator recruited to PP7 binding loops in the tracrRNA of the 
            guide)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  TITLE     Schmierer unpublished plasmids
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CROPSeq-PP7-p65-HSF
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(3375..3399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3449..3469
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3485..4027
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4052..4423
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             4439..4492
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4514..5113
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4514..4533)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5010..5030
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5129..5717
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5182..5202)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5600..5611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5873..6121
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5873..5893
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6044..6063
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             6362..6542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6620..6741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6657..6676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6711..6730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6781..6916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6936..6955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6937..6955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6965..6982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6965..6981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7123..7578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7210..7229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7420..7441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7604..7708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7709..7777,7778..8569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7927..7946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8740..9328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9229..9248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9482..9499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9616..9637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9652..9657,9658..9675,9676..9682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9690..9706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9695..9717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9714..9730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9714..9730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9749..9769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9751..9769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9797..10023
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgtc
     2941 gcggaggcgg cacagctacg agaacgatgg tggacaacct cacaaaagga ggaagacgtc
     3001 tatgctagcc tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga
     3061 gatccagtcc accgcagacc gtcagatctt cgaagagaag gtcgggcctc tggtgggtcg
     3121 gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gagtcaacct
     3181 aaaactggat caggcggacg tcgttgattc cggacttccg aaagtgcgct acactcaggt
     3241 atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta
     3301 cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa gatcttgtcg tcaaccttgt
     3361 gccgctgggc cgttcttcaa ctttcttgta caaagtgggc ggaggaggtg gaagcggagg
     3421 aggaggaagc ggaggaggag gtagcggacc taagaaaaag aggaaggtgg cggccgctgg
     3481 taccccttca gggcagatca gcaaccaggc cctggctctg gcccctagct ccgctccagt
     3541 gctggcccag actatggtgc cctctagtgc tatggtgcct ctggcccagc cacctgctcc
     3601 agcccctgtg ctgaccccag gaccacccca gtcactgagc gctccagtgc ccaagtctac
     3661 acaggccggc gaggggactc tgagtgaagc tctgctgcac ctgcagttcg acgctgatga
     3721 ggacctggga gctctgctgg ggaacagcac cgatcccgga gtgttcacag atctggcctc
     3781 cgtggacaac tctgagtttc agcagctgct gaatcagggc gtgtccatgt ctcatagtac
     3841 agccgaacca atgctgatgg agtaccccga agccattacc cggctggtga ccggcagcca
     3901 gcggcccccc gaccccgctc caactcccct gggaaccagc ggcctgccta atgggctgtc
     3961 cggagatgaa gacttctcaa gcatcgctga tatggacttt agtgccctgc tgtcacagat
     4021 ttcctctagt gggcagggag gaggtggaag cggcttcagc gtggacacca gtgccctgct
     4081 ggacctgttc agcccctcgg tgaccgtgcc cgacatgagc ctgcctgacc ttgacagcag
     4141 cctggccagt atccaagagc tcctgtctcc ccaggagccc cccaggcctc ccgaggcaga
     4201 gaacagcagc ccggattcag ggaagcagct ggtgcactac acagcgcagc cgctgttcct
     4261 gctggacccc ggctccgtgg acaccgggag caacgacctg ccggtgctgt ttgagctggg
     4321 agagggctcc tacttctccg aaggggacgg cttcgccgag gaccccacca tctccctgct
     4381 gacaggctcg gagcctccca aagccaagga ccccactgtc tccgatatcg gcagtggaga
     4441 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caggtaccac
     4501 agatccaccg gagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4561 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4621 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4681 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     4741 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4801 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4861 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     4921 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4981 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5041 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5101 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5161 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5221 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5281 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5341 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5401 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5461 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5521 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5581 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5641 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5701 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     5761 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5821 gatttcacca ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct
     5881 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     5941 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     6001 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     6061 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     6121 cggagacggt tgttaattaa tgtacgtctc tgtttaagag ctatgctgcg aatacgaggg
     6181 agcagacgat atggcgtcgc tcctctccac gagagcatat gggctccgtg gctcgtattc
     6241 gcagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     6301 cggtgctttt tttgaattct tggcgtaact agatcttgag acactgcttt ttgcttgtac
     6361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6541 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6601 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     6661 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6721 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     6781 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6841 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     6901 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     6961 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7021 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7081 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7141 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7201 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7261 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7321 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7381 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7441 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7501 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7561 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7681 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7741 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7801 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7861 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7921 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7981 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8041 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8101 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8161 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8221 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8281 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8341 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8401 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8461 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8521 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8581 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8641 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8701 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8821 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8881 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8941 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9001 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9061 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9121 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9181 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9241 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9301 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9361 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9421 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9481 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9601 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9661 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9721 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     9781 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     9841 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     9901 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     9961 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
    10021 aac
//
