LOCUS       sequence-388080-        7264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Epitope tagging of effector protein SseF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7264)
  TITLE     Hensel lab plasmids
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p2095
FEATURES             Location/Qualifiers
     source          1..7264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1297..2247)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2295..2517
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     3662..3679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3796..3817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3832..3837,3838..3855,3856..3862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3870..3886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3875..3897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3894..3910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3894..3910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3929..3949
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3931..3949
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    4007..4128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4018..4037)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4072..4091
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5866..5882)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5867..5883)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5907..5926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5908..5926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5936..5953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5936..5952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5945..5967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6094..6549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6181..6200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6391..6412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6575..6679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6680..6748,6749..7264,1..276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6898..6917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
       61 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      121 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      181 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      241 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      301 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      361 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagat
      421 ctctgttcgg gggttcactg acgctgcgtg actacgatgg tcaggttgcg gaggctatgg
      481 ccctggtacg agcgctgaac aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg
      541 cctgaaaaca caacccgcta cgggggagac ttacccgaaa tctgatttat tcaacaaagc
      601 cactttcagt atggatttgg gtgttacggg ggggggttgg tgcttaaaat cgcgctgagg
      661 atttctgatg gtgttaagcg ggcggttttg agatgtaaac tcgcccgttt aacataatgg
      721 atcttgcgcg caccgcccga acaccactcg ccacaaaaaa ccgccggaac gtccaaaagt
      781 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
      841 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
      901 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
      961 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
     1021 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
     1081 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
     1141 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     1201 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     1261 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     1321 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     1381 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     1441 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     1501 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     1561 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     1621 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     1681 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     1741 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     1801 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     1861 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     1921 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     1981 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     2041 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     2101 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     2161 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     2221 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     2281 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     2341 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     2401 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     2461 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     2521 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     2581 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     2641 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     2701 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     2761 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     2821 ggcgttttat ggcgggtctg cggagtggtg aatccgttag cgaggtgccg ccggcttcca
     2881 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     2941 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     3001 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     3061 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     3121 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     3181 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     3241 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     3301 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     3361 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
     3421 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     3481 ttgaaggctc tcaagggcat cggcattcgc cattcaggct gcctcctacc aagacgactt
     3541 caaatccgac ccgtagagat ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3661 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3721 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3781 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3841 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3901 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     3961 ggagctccac cgcggtggcg gccgctctag agtcgaccag acatgataag atacattgat
     4021 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4081 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttct gctttaataa
     4141 gatcctagaa ctagaggatc cgcgtctctg tctcaaaagg aggtaggcga tccctactcc
     4201 gatccatggc accaccccca tctccccgag atgtatgatc agaactgtcc attaatgcag
     4261 gtgtagtagc agattgacag agcgctgcca tttgttccgc caataacaca ctgggatcgg
     4321 catgaagttc atcaacagaa ttttcctgat cgtcgccaga ggggcgggca aggcaataat
     4381 ccagtaccgc acctatcgcc gtcaggctaa cggaggtaat tacactcccc atgtccaaag
     4441 aggccgcaat attttcagcc gcgggcagtg gaaactgtag gggtaaaacc aacatagaaa
     4501 tagcgattcc tgaacgtatt aataaagaaa gacaattagc aagggtgtta gcgcagttaa
     4561 gacttgcccc acattttaag gccagcgcac tgaccacaag agcaacgcta tcactggcgg
     4621 tttgtaatgg ctccttttgc tgacatatcg attgataatt atgatacgca cagcaagcat
     4681 ccccaatagc aatcacgagc gccgcccccg caagaatagc aatgggtaat cctgccccgc
     4741 cagaaattac cgctgcagca accgataacc caaacacgac tgtcgcaccc agcgcacgaa
     4801 tagtgtattg cataaaatgt atcgcataat ccctctgctg ccttatttgt tcaggcgtaa
     4861 gcagcacagg ggctgcgggg gtgccaggcg ctggaatttc agggggagga aacgatacct
     4921 ccttcgcttg tatatcggaa ggaggactat taccatcgac tatattactt gccgctgacg
     4981 gaatatgaat tttcatattt cgttctgtta tttaagcaat aagagtatca accattattt
     5041 gcgcattctg gcgaatctca ctccatgagg catccgcata actcatcttg attgcggttt
     5101 gaaaagcctc tctcgccaac ccgggttccc ccatcatttt gagacagacg cccgtttggt
     5161 aaaccggttc tggatggctg gcatccagca tcaaggcatg tccatagaaa ttaatggccg
     5221 tcgtgtattc tttaagcatc atccaggtgc cagccaatgc aatatgggca cgccaactcc
     5281 atggctgggc catcaccagc caactaaaat cgattacggc gcgcgaataa tccccctcct
     5341 gccatgaggc gtaaccactg gcataaacgg tttccggatc aacggataat agctgtttca
     5401 gaatgtcttc gggtatttta tttttctgat cttctttcat catcatacct attgaattca
     5461 cgataagata attaacgtgc taactaagag cctatcccat tagggctatt ttacttgcca
     5521 ttttgaacct gggcagtgct cgccaaaacg cgttagcgtt ttgaacgccg ctagcggcgg
     5581 cccgaagggc gagcgtagcg agtcaaacct cacgtactac gtgtacgctg cggtttttgc
     5641 gcgctatccg tgtccaaact ggctgcgcca attaacgcct ggtgggatag gctctaagat
     5701 atttttactt tactcttgct cactcactac aagtgcgctg ttatggtaac gataataaat
     5761 aatgttgatg atattttcgg cctgctcgat cgcttcaagc aataaggtgt tttgctgata
     5821 ttgctgcgga tcctgtaact tgccgttgtt ctcttctaag cttatcgata ccgtcgacct
     5881 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     5941 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6001 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6061 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6121 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6181 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6241 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6301 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6361 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6541 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6901 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7141 ggatcatgta actcgccttg atcgctggga accggagctg aatgaagcca taccaaacga
     7201 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7261 cgaa
//
