LOCUS       sequence-388089-        8165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of LAMP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8165)
  TITLE     Hensel lab plasmids
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p3991
FEATURES             Location/Qualifiers
     source          1..8165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(918..1001,1002..2168)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             2208..3092
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             join(3156..3158,3159..3161,3162..3875)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3201..3222)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3462..3481)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3809..3830
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          3945..4174
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4157..4176
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4354..4816
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4410..4427)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4637..4656
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4852..5655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4915..4934)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5525..5544
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5785..5906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5822..5841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6008..6024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6008..6024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6021..6043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6032..6048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6056..6062,6063..6080,6081..6086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6101..6122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6239..6256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6410..6998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6490..6509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7169..7960,7961..8029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7792..7811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8030..8134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatcatg gcggcccccg gcagcgcccg gcgacccctg ctgctgctac
      961 tgctgttgct gctgctcggc ctcatgcatt gtgcgtcagc agcaatgttt atggtgaaaa
     1021 atggcaacgg gaccgcgtgc ataatggcca acttctctgc tgccttctca gtgaactacg
     1081 acaccaagag tggccctaag aacatgacct ttgacctgcc atcagatgcc acagtggtgc
     1141 tcaaccgcag ctcctgtgga aaagagaaca cttctgaccc cagtctcgtg attgcttttg
     1201 gaagaggaca tacactcact ctcaatttca cgagaaatgc aacacgttac agcgtccagc
     1261 tcatgagttt tgtttataac ttgtcagaca cacacctttt ccccaatgcg agctccaaag
     1321 aaatcaagac tgtggaatct ataactgaca tcagggcaga tatagataaa aaatacagat
     1381 gtgttagtgg cacccaggtc cacatgaaca acgtgaccgt aacgctccat gatgccacca
     1441 tccaggcgta cctttccaac agcagcttca gcaggggaga gacacgctgt gaacaagaca
     1501 ggccttcccc aaccacagcg ccccctgcgc cacccagccc ctcgccctca cccgtgccca
     1561 agagcccctc tgtggacaag tacaacgtga gcggcaccaa cgggacctgc ctgctggcca
     1621 gcatggggct gcagctgaac ctcacctatg agaggaagga caacacgacg gtgacaaggc
     1681 ttctcaacat caaccccaac aagacctcgg ccagcgggag ctgcggcgcc cacctggtga
     1741 ctctggagct gcacagcgag ggcaccaccg tcctgctctt ccagttcggg atgaatgcaa
     1801 gttctagccg gtttttccta caaggaatcc agttgaatac aattcttcct gacgccagag
     1861 accctgcctt taaagctgcc aacggctccc tgcgagcgct gcaggccaca gtcggcaatt
     1921 cctacaagtg caacgcggag gagcacgtcc gtgtcacgaa ggcgttttca gtcaatatat
     1981 tcaaagtgtg ggtccaggct ttcaaggtgg aaggtggcca gtttggctct gtggaggagt
     2041 gtctgctgga cgagaacagc atgctgatcc ccatcgctgt gggtggtgcc ctggcggggc
     2101 tggtcctcat cgtcctcatc gcctacctcg tcggcaggaa gaggagtcac gcaggctacc
     2161 agactatccg ggatccaccg gtcgccaccg aattccatat gggatccgaa atcggtactg
     2221 gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg
     2281 ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg
     2341 tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga
     2401 tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct
     2461 tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact
     2521 ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg
     2581 catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg
     2641 agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg
     2701 tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg
     2761 accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa
     2821 acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg
     2881 actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga
     2941 tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca
     3001 tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg
     3061 cgcgctggct gtctactctg gagatttccg gtcctgcagg tataggcgcg ccaggatccg
     3121 gccatggcac aggctcaaca ggctcaggct catcaatggt gagcaagggc gaggagctgt
     3181 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3241 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3301 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3361 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3421 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3481 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3541 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3601 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3661 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3721 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaactga
     3781 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3841 ggatcactct cggcatggac gagctgtaca agtgaggcgg ccgcatagat aactgatcca
     3901 gtgtgctgga attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc
     3961 aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat
     4021 attcacctgg cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac
     4081 aatctttttg ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt
     4141 gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag
     4201 gtcgagcatg catctagggc ggccaattcc gcccctctcc cccccccccc cctccccccc
     4261 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     4321 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4381 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     4441 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     4501 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     4561 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     4621 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     4681 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     4741 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     4801 aaaaacacga tgataagctt gccacaaccc gggataattc ctgcagccaa tatgggatcg
     4861 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4921 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4981 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5041 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5101 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5161 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5221 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5281 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5341 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5401 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5461 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5521 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5581 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5641 gacgagttct tctgagggga tcaattctct agataactga tcataatcag ccataccaca
     5701 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5761 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5881 ttgtccaaac tcatcaatgt atcttaacgc gtcgagtgca ttctagttgt ggtttgtcca
     5941 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8161 acgtc
//
