LOCUS       sequence-38811-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Ayaydin F, Dasso M
  TITLE     Distinct in vivo dynamics of vertebrate SUMO paralogues.
  JOURNAL   Mol Biol Cell. 2004 Dec . 15(12):5208-18.
  PUBMED    15456902
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2004 Dec . 15(12):5208-18."
COMMENT     Sequence Label: pEYFP SUMO-1
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(547..798)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(589..610)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagatccgg tggatcccgg gcccgcggta ccgtcgagca gtcagtcacg atgaattgcc
      181 cgatccacta gtaacggccg ccagtgtgct ggaattcggc ttattctaaa ctgttgaatg
      241 accccccgtt tgttcctgat aaacttcaat cacatcttct tcctccattc ccagttcttt
      301 tggagtatga ttatcagcaa ttctctgacc ctcaaagaga aacctgagtg aattcattgg
      361 aacaccctgt ctttgacagt atgattcttt gagtttcttg agatgtgttg tcattttcac
      421 tttgaagtga atctcactgc tatcctgtcc aatgactttg agtttaatat attcaccttc
      481 cttcttatcc cccaagtcct cagttgaagg ttttgcctcc tggtcagaca tggatctgag
      541 tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      601 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      661 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      721 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      781 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg c
//
