LOCUS       sequence-38812-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Lindsey-Boltz LA, Sancar A
  TITLE     Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase 
            II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates 
            Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation
            of Checkpoint Kinase 1 (Chk1).
  JOURNAL   J Biol Chem. 2011 Jun 3. 286(22):19229-36.
  PUBMED    21502314
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jun 3. 286(22):19229-36."
COMMENT     Sequence Label: pcDNA5-FRT/TO-LacR-TopBP1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             312..332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             345..368
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgcatgcgc gccattaccg agtccgggct gcgcgtttgg tgcggatatc tcggtagtgg
       61 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      121 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
      181 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
      241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      301 aggtttcccg acccaagaag aagaggaagg tgggatccac catggattac aaagacgatg
      361 acgacaagtc cagaaatgac aaagaaccgt tttttgtgaa gtttttaaag tcttcagaca
      421 attccaaatg tttttttaaa gctctcgagt ccataaaaga attccaatca gaagaatatc
      481 ttcagattat tacagaagaa gaggcattga agataaagga gaatgataga tcactttata
      541 tctgtgaccc ttttagtggc gttgtctttg atcacctcaa aaagcttggc tgcagaattg
      601 ttggtcctca agtagtcata ttttgtatgc accaccagcg atgtgtccca agagccgaac
      661 atccagttta taatatggtt atgtctgatg taaccatatc ttgtacaagt ctggaaaaag
      721 aaaaaaggga agaagttcat aaatatgtac aaatgatggg cggacgagta tacagagacc
      781 ttaatgtatc agtaactcac cttattgcag gagaagttgg tagcaaaaat atttagttgc
      841 tgcaaacctg aagaaaccta ttttgcttcc ctcttggata aaaacacttt gggagaagtc
      901 acaggagaaa
//
