LOCUS       sequence-388123-        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Cavbeta4 calcium 
            channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CavB4/pFlag myc-CMV
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             510..533
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             546..575
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    588..612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2173..2197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2334..2468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2371..2390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2425..2444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2491..2507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2491..2507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2504..2526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2515..2531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2539..2545,2546..2563,2564..2569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2721..2738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2892..3480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2972..2991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3651..4442,4443..4511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4274..4293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4721..4739
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4777..4799)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4899..4918
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5150..5172
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5164..5181
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5165..5181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5747..6126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6127..6330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6281..6301
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6327..6351
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          join(6466..6827,1..465)
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
ORIGIN
        1 acgtgggatc tccacgcgaa tctcgggtac gtgttccgga catgggctct tctccggtag
       61 cggcggagct tctacatccg agccctgctc ccatgcctcc agcgactcat ggtcgctcgg
      121 cagctccttg ctcctaacag tggaggccag acttaggcac agcacgatgc ccaccaccac
      181 cagtgtgccg cacaaggccg tggcggtagg gtatgtgtct gaaaatgagc tcggggagcg
      241 ggcttgcacc gctgacgcat ttggaagact taaggcagcg gcagaagaag atgcaggcag
      301 ctgagttgtt gtgttctgat aagagtcaga ggtaactccc gttgcggtgc tgttaacggt
      361 ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag
      421 ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtccttgaca
      481 cgaagcttcc cgggataggt accaccatgg actacaagga cgatgacgat aaatcgcgag
      541 atctcgagca gaaactcatc tctgaagagg atctgagagt cgaatcaaca agtttgtaca
      601 aaaaagcagg cttctcgtcc tcctacgcca agaacggggc ggcggacggg ccgcactccc
      661 ccagctcgca ggtggcccga ggcaccacga cccggaggag caggttgaaa agatccgatg
      721 gcagcaccac ttccaccagc ttcatcctca gacaggggtc agcagattcc tatacaagca
      781 ggccatcgga ctccgatgtc tctttggaag aggaccggga agcgattcgg caagagagag
      841 aacagcaagc agctatccag cttgagagag cgaagtccaa acctgtagct tttgcggtga
      901 aaacgaatgt gagctactgt ggtgccctgg atgaggatgt gcccgttccc agcacagcca
      961 tctcctttga cgccaaggac tttcttcaca ttaaagagaa atataacaat gattggtgga
     1021 taggaaggct ggtgaaggag ggctgtgaga tcggcttcat cccaagtcca ctgcgtttgg
     1081 agaacatacg gattcaacag gaacagaaaa gaggccgttt tcatggcggg aaatcgagtg
     1141 gaaactcttc ctccagtctg ggagaaatgg tgtcaggaac atttcgagca actcccacaa
     1201 caacagcaaa acagaagcag aaagtgacgg agcacattcc tccgtatgat gttgtgccat
     1261 caatgcgtcc tgtggtgtta gtagggccat cactgaaagg ttatgaggta acagacatga
     1321 tgcagaaagc cctctttgat ttcctgaagc acaggtttga tgggaggata tcaataacca
     1381 gagtgacagc tgacatttct cttgctaaga ggtctgtcct caacaatcct agcaagagag
     1441 caataattga acgttcaaac accagatcca gcctagcgga agtacagagt gaaattgaaa
     1501 gaattttcga gttggcaaga tctttgcaat tggttgttct cgatgcagac accatcaatc
     1561 acccagcaca gctgataaag acatcgttag cgcccatcat cgtccacgtg aaggtctcgt
     1621 ccccaaaggt tttacagcgg ctgattaagt ccagaggaaa gtcccagagt aaacacttga
     1681 atgttcaact ggtggcagct gataaactgg cccagtgtcc ccctgaaatg tttgatgtta
     1741 tattagatga gaatcaactt gaggatgcct gtgaacatct gggagagtac ctggaggcat
     1801 attggcgtgc cacccacaca agcagtagca cccctatgac cccattgctg gggaggaacg
     1861 tgggctccac agctctctca ccgtatccca cagcaatctc tggactacag agtcagcgga
     1921 tgaggcatag caaccactct acagagaatt ctccaattga aagacgaagc ctaatgacct
     1981 ctgatgaaaa ttaccacaat gagagggccc gcaagagtag gaaccgcttg tcctccagct
     2041 cccagcacag ccgagatcac taccctctgg tggaagaaga ttatcctgac tcataccagg
     2101 acacttacaa accccatagg aaccgaggct cacctggggg atgcagccat gactcccgac
     2161 ataggcttta ggacccagct ttcttgtaca aagtggttga ttcgacctgc aggatatcga
     2221 attcattgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2281 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2341 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2401 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2461 tctggatccg taccgagctc gcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     2521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2581 ctaatgagtg agctaactac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4501 tgaatactca tactcttcct ttttcaataa gcggccgcgg ccatgccggc cactagtctc
     4561 gagttattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4621 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4681 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4741 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4801 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4861 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4921 gagagtgcac catatgaaga cgtcgcctcc tcactacttc tggaatagct cagaggccga
     4981 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ggcgccattc gccattcagg
     5041 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5101 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     5161 cgttgtaaaa cgacggccag tgcccgtgtc aaggacggtg actgcagtga ataataaaat
     5221 gtgtgtttgt ccgaaatacg cgttttgaga tttctgtcgc cgactaaatt catgtcgcgc
     5281 gatagtggtg tttatcgccg atagagatgg cgatattgga aaaatcgata tttgaaaata
     5341 tggcatattg aaaatgtcgc cgatgtgagt ttctgtgtaa ctgatatcgc catttttcca
     5401 aaagtgattt ttgggcatac gcgatatctg gcgatagcgc ttatatcgtt tacgggggat
     5461 ggcgatagac gactttggtg acttgggcga ttctgtgtgt cgcaaatatc gcagtttcga
     5521 tataggtgac agacgatatg aggctatatc gccgatagag gcgacatcaa gctggcacat
     5581 ggccaatgca tatcgatcta tacattgaat caatattggc cattagccat attattcatt
     5641 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tccatatcat
     5701 aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg
     5761 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5821 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5881 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5941 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6001 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6061 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6121 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     6181 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     6241 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     6301 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     6361 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccgcggc
     6421 cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat
     6481 agagtctata ggcccacccc cttggcttct tatgcatgct atactgtttt tggcttgggg
     6541 tctatacacc cccgcttcct catgttatag gtgatggtat agcttagcct ataggtgtgg
     6601 gttattgacc attattgacc actcccctat tggtgacgat actttccatt actaatccat
     6661 aacatggctc tttgccacaa ctctctttat tggctatatg ccaatacact gtccttcaga
     6721 gactgacacg gactctgtat ttttacagga tggggtctca tttattattt acaaattcac
     6781 atatacaaca ccaccgtccc cagtgcccgc agtttttatt aaacata
//
