LOCUS       sequence-388130-        3370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#78.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM43
FEATURES             Location/Qualifiers
     source          1..3370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1257..1356)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1413..1429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1413..1429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1426..1448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1601..2189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1681..1700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2325..3140)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2325..3140)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3048..3067
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3132..3151
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3261..3277
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3261..3277
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3278..3324
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gagtagaaga aaaagggggg gtcggcctaa
      301 aagtaaatac tggagtgaag ctgaaaaatt agaaggtaaa aatatgtgga agtgcaacca
      361 ttgtaaacat gaatttgccg gaggtgctac aagaattgaa gagcatatca ctggcaaagg
      421 aaacaatatt acaaaatgcc caaaatatgg tgcccatgaa gaagatgaaa ttaacttggg
      481 agaccagata gtggagcatg attctgctgc atttaaaaag gcgtatccaa agggggaccc
      541 tgatgctgtt tgtatcaaaa agagcgatat tgagctttta cagcctgaga aatgccttaa
      601 tgatactatc attgactttt atatcaatta tttgaaaaat aaacttccaa gcgataaaca
      661 agacaggttt cactttttca attgcttttt ctttgctaag cttgctggtt taagcagaaa
      721 tgatacatca attgcttgtg atggtaaagc agcatttcag cgtgtaagca cttggggaag
      781 aaaagtcaac ctttttaaaa cggattatat cttcattccc ataaactata gtcttcactg
      841 gagtttgatt gtcatttgtc accctgctga agtcatgaca tgctacagag atgaagaaac
      901 taagggatct cccaaagaag cttgcatctt gcacatggat tcccgaaaag gaattcatgt
      961 acatctccag aatgttttcc aaagttatct atgtgaagaa tggaaagaga ggcacaacaa
     1021 tgtgagggat gatgtttctc ctaaattttt agatcttcca ttcgtgccac ttgagctgcc
     1081 tcagcaacaa aatgcatacg attgtggcat ctttttgctc cactatgtgg aacattttct
     1141 ggaacaagct ccaatcaact ttaaccgttc aaccaagttc agtgttcctc caccagatgc
     1201 ttctctgaaa cgatctcata tacagaaact attaatagcg gctgaaggag acagtgaccc
     1261 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1321 acaggtcact atcagtcaaa ataaaatcat tatttgaggc taggtggagg ctcagtgatg
     1381 ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt
     1441 tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt
     1501 ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc
     1561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2221 acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca
     2281 aatcgtccgc tctgccagtg ttacaaccaa ttaacaaatt ctgattagaa aaactcatcg
     2341 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2401 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     2461 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2521 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2581 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     2641 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2701 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2761 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2821 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2881 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2941 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     3001 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     3061 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt
     3121 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     3181 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     3241 acgtggcttt cccccgccgc tctagaacta gtggatccaa ataaaacgaa aggctcagtc
     3301 gaaagactgg gcctttcgtt ttatctgttg tttgtcgcat tatacgagac gtccaggttg
     3361 ggatacctga
//
