LOCUS       sequence-388144-        7643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the GluR1/GluA1 AMPA 
            receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7643)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GluR1-Flop/pRK5
FEATURES             Location/Qualifiers
     source          1..7643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(30..35,36..53,54..60)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    68..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     73..95
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     92..108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     92..108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..720
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     671..691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     717..741
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        951..969
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     951..968
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     982..1001
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1167..1178
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4066..4075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4079..4213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4116..4135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4170..4189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4282..4639
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4282..4473
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4305..4325)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4490..4625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4659..4676)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4659..4675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4668..4690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4888..5343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5185..5206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5360..5379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5479..5501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5539..5557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5625..5729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5730..5798,5799..6590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5948..5967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6761..7349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7250..7269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7503..7520
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(7637..7643,1..15)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
      121 taattcgagc tcgcccgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      541 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      601 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      661 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      721 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      781 agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc
      841 cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg
      901 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
      961 cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac
     1021 tgcacctcgg ttctatcgat tgaattcggc acgagctcgg ctccccttcc aagagaaaca
     1081 agagaaacct cacggaagga agggaggaag gaaagaagca agcaaggaac tgcaggaaga
     1141 aaagagccgg cagagcatca agaagaatcg aagggagggg agggaagacc aaatctatgg
     1201 ttggaccagg gcttcttttt cgccaatgta aaaaggaata tgccgtacat ctttgccttt
     1261 ttctgcaccg gttttctagg tgcggttgtg ggtgccaatt tccccaacaa tatccagata
     1321 ggggggttat ttccaaacca acaatcacag gaacatgcgg cttttaggtt tgctttgtca
     1381 caactcacgg agccccccaa gctgcttccc cagatcgata ttgtgaacat cagcgacagc
     1441 tttgagatga cttaccgttt ctgttcccag ttctccaaag gagtctatgc catctttgga
     1501 ttttatgaac gaaggactgt caacatgctg acctccttct gtggggccct ccatgtgtgc
     1561 ttcattactc caagttttcc tgttgacaca tccaatcaat ttgtccttca gctacgcccg
     1621 gaactacagg aagctctcat tagcattatc gaccattaca agtggcaaac ctttgtctac
     1681 atttatgatg ctgaccgggg cctgtcagtc ctgcagagag tcttggatac agccgcagag
     1741 aagaactggc aggtaacggc tgtcaacatt ctgacaacca ccgaggaagg ataccggatg
     1801 ctctttcagg acctggagaa gaaaaaggag aggctggtgg tggttgactg tgaatcagaa
     1861 cgcctcaacg ccatcctggg ccagatcgtg aagctagaaa agaatggcat cgggtaccac
     1921 tacatcctcg ccaatctggg cttcatggac attgacttaa ataagttcaa ggagagcgga
     1981 gccaatgtga caggtttcca gctggtgaac tacacagaca cgatcccagc cagaatcatg
     2041 cagcaatgga ggacaagtga ctcccgagac cataccaggg tggactggaa gaggccaaag
     2101 tacacttctg ctctcaccta tgatggtgtc aaggtgatgg ctgaggcctt ccaaagcctg
     2161 cggaggcaga ggattgacat atcccgccgg gggaatgctg gggactgtct ggctaaccca
     2221 gctgtgccct ggggtcaagg gatcgacatc cagagagccc tgcagcaggt gcgcttcgaa
     2281 ggtttgacag gaaatgtgca gttcaacgag aaagggcgcc ggaccaatta caccctccac
     2341 gtgatcgaaa tgaaacatga tggaatccga aagattggtt actggaatga agacgataaa
     2401 tttgtccccg cagccaccga cgctcaggct ggaggggaca actcaagcgt ccagaatagg
     2461 acctacatcg tcactactat cctcgaagat ccttacgtga tgcttaaaaa gaatgccaac
     2521 cagtttgagg gcaatgaccg ctatgagggc tactgtgtgg agctggctgc agagatcgcc
     2581 aagcacgtgg gctactccta ccgacttgag attgtcagcg acggcaaata tggagcccgg
     2641 gatcccgaca caaaggcttg gaatggcatg gtgggagaac tggtctatgg aagagcagac
     2701 gtggctgtgg ctcccttgac cataaccttg gtccgggagg aagtcatcga cttctccaag
     2761 ccattcatga gtttgggaat ctccattatg attaagaagc cacagaagtc caagccaggt
     2821 gtcttctcct ttcttgaccc tttggcctat gagatctgga tgtgtatagt gtttgcctac
     2881 attggagtga gcgtcgtcct cttcctggtc agccgtttca gcccctacga atggcacagc
     2941 gaagagtttg aagagggacg agaccagaca accagtgacc agtcaaatga gtttggcata
     3001 ttcaacagcc tgtggttctc cctgggggcc ttcatgcagc aaggatgtga catttccccc
     3061 aggtccctgt ccggacgcat cgtcggcggc gtctggtggt tcttcacttt gatcatcatc
     3121 tcctcgtaca cagccaacct ggctgccttc ctgactgtgg agaggatggt gtctcccatt
     3181 gagagtgcag aggacctggc aaagcagacg gaaattgctt atgggacatt ggaagcaggc
     3241 tccactaagg agttcttcag gagatctaaa atcgctgtgt ttgagaagat gtggacatac
     3301 atgaagtctg cagaaccatc cgtgtttgtt cggaccacag aggaaggcat gatcagagtg
     3361 agaaaatcta aaggcaaata cgcctacctc ctggagtcca ccatgaatga gtatattgag
     3421 caacgaaagc cctgtgacac catgaaagtg ggaggtaact tggattccaa aggctatggc
     3481 attgcgacac ccaaggggtc cgccctgaga aatccagtaa acctggcagt gttaaaactg
     3541 aacgagcagg ggcttttgga caaattgaaa aacaaatggt ggtacgacaa gggcgagtgc
     3601 ggcagcgggg gaggtgactc caaggacaag accagcgctt tgagcctcag caatgtggca
     3661 ggcgtgttct acatcctgat tggagggctg ggactggcca tgctggttgc cttaatcgag
     3721 ttctgctaca aatcccgtag cgagtcgaag cggatgaagg gtttctgttt gatcccacag
     3781 caatccatca atgaagccat acggacatcg accctccccc ggaacagtgg ggcaggagcc
     3841 agcggaggag gcggcagtgg agagaatggc cgggtggtca gccaggactt ccccaagtcc
     3901 atgcaatcca ttccctgcat gagtcacagt tcagggatgc ccttgggagc cacaggattg
     3961 taactggagc agacaggaaa cccttgggga gcaggctcag gcttccacag ccccatccca
     4021 agcccttcag tgccaaaaaa aaaaaaaaaa aactcgagaa gcttggccgc catggcccaa
     4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4201 tcatgtctgg atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag
     4261 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     4321 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4381 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4441 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4501 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4561 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4621 aggcctaggc ttttgcaaaa agctgttaac agcttggcac tggccgtcgt tttacaacgt
     4681 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4741 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4801 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4861 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5221 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5341 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5401 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5461 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5521 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5581 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7381 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7441 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7501 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7561 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7621 gtgagcgcaa cgcaattaat gtg
//
