LOCUS       sequence-388154-        5091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting FGF14/FHF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5091)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FGF14-Myc
FEATURES             Location/Qualifiers
     source          1..5091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    419..440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(455..460,461..478,479..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    493..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     498..520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     517..533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     517..533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     552..572
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    685..819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(722..741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     776..795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(883..912)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(946..975)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(985..1014)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1024..1053)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(1063..1092)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(1909..1927)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1910..1927)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1962..2946)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      3017..3472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3104..3123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3314..3335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3498..3602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3603..3671,3672..4463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3821..3840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4634..5091,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      421 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctc ccggctcgta
      481 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      541 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggcccaatg
      601 cattggcgcc gcggccgcga attaaaaaac ctcccacacc tccccctgaa cctgaaacat
      661 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      781 ttgtccaaac tcatcaatgt atcttatcat gtctggatct acgtaatacg actcactata
      841 gttctagaag atctcactta cctattcgag aggccttgaa ttcaagtcct cttcagaaat
      901 gagcttttgc tccatggtga ggtcgcccaa gctctccatt tcattcaagt cctcttcaga
      961 aatgagcttt tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatttc
     1021 attcaagtcc tcttcagaaa tgagcttttg ctccatttca ttcaagtcct cttcagaaat
     1081 gagcttttgc tccatagctt taaatcgatg ggatcctgtt gtcttactct tgttgactgg
     1141 tttgcctcca ttcattattg cagacgcact tgtgctttta cttggcgtca ccccaggctt
     1201 cgggaccgtt tccccaacat catgcaaaga tggttctcgg tacatggcaa cttccaatgg
     1261 cttgggtaga aaatgagctg ctggtttggt tttctttact ctgttccctt tcatagcttg
     1321 cccttcctta tttaatccca aaaaccaggc tctaccagat tcctgttgtc tgtacaacat
     1381 ggatgagtag attacataat aattttcaaa aacagattct ttaaacttgc attcaggggt
     1441 aaaaagttct gatgggtaga ggtaaccttc tccattcatg gctatataca accctgtttt
     1501 cactccctgg atggcaacaa cacgtagtcc cactggtatg aggttgaaga gtgtagaatt
     1561 agtgctgtca tccttggttc catcgagagc tccatcgggg tgcatttgca agtagtagcc
     1621 ttgcctgcaa tataacctgg tcactatacc cttgagctgg ggatcagttg ggttcttgtt
     1681 tttcttaaga ctcttgccac aaagacactg cagcatatgc gttcctttgc tgaaaatgtt
     1741 ccaaagaaac cacattgatt tgggcgaaaa gcaatccagc agcttagaca ccctgagaaa
     1801 gaagagatcc ttgtggttgc ataataataa tttgaaatca gttctcctga agaggggcac
     1861 cggttttacc atggtggaga tcctgcaaaa agaacaagta gcttgtattc tatagtgtca
     1921 cctaaatcaa gcttgctccg acgtccccag gcagaatggc ggttccctaa acgagcattg
     1981 cttatataga cctcccatta ggcacgccta ccgcccattt acgtcaatgg aacgcccatt
     2041 tgcgtcattg cccctcccca ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc
     2101 atttaccgcc attgacgtca atgggagtac tgccaatgta ccctggcgta cttccaatag
     2161 taatgtactt gccaagttac tattaataga tattgatgta ctgccaagtg ggccatttac
     2221 cgtcattgac gtcaataggg ggcgtgagaa cggatatgaa tgggcaatga gccatcccat
     2281 tgacgtcaat ggtgggtggt cctattgacg tcaatgggca ttgagccagg cgggccattt
     2341 accgtaattg acgtcaatgg gggaggcgcc atatacgtca ataggaccgc ccatatgacg
     2401 tcaataggaa agaccatata tagagaccat tgacgtcaat gggggagtgg ctatgggcgg
     2461 tattaggaag ccccatatat ggtatatggg accgcccatt gggaggggct atctacgtca
     2521 ataggaaaac ccatatatgg aatactatat ggcatagggc caatacatag tattgaacct
     2581 ggccaatagc catattggca tagggccata ttggatattg cctatatatt gatcctggca
     2641 tatagccaat atggccgcca ttattggcac catgccaatt caatatggcg gacttggcac
     2701 agggccaaga cccctcctca aacttggcac cgtgccaagt agacccctcc ccagttggca
     2761 cagtgccagg tccgccatat tgaattggca tggtgccaag tccgccatat tgaattggct
     2821 ggggtccaga tccaccatat tgaattggca cgagtccata tacgccatat tgaattggca
     2881 caggtccaga tccaccatat tgaattggca tgagtccata tacgccatat tgaattggcg
     2941 gtcgactggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3001 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3301 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3361 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3421 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc g
//
