LOCUS       sequence-388188-       10404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  study roles of human BET proteins in human pluripotency 
            reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Hossain ME, Cevallos RR, Zhang R, Hu K
  TITLE     Attenuating iPSC reprogramming stress with dominant-negative BET 
            peptides.
  JOURNAL   iScience. 2022 Dec 28;26(1):105889. doi: 10.1016/j.isci.2022.105889.
            eCollection 2023 Jan 20.
  PUBMED    36691621
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-12-28"; volume: "26(1):105889. doi"; pages: " 
            10.1016/j.isci.2022.105889. eCollection 2023 Jan 20"
COMMENT     Sequence Label: pLV-EF1a-AcGFP-P2A-hBRD4S
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2409..3347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3304..3324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3370..4086
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             4114..4170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6372..6960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6425..6445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6843..6854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7167..7800
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7929..7945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7929..7945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7942..7964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7953..7969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7977..7983,7984..8001,8002..8007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8022..8043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8160..8177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8331..8919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8411..8430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9090..9881,9882..9950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9713..9732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9951..10055)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10103..10237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10140..10159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10194..10213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg
     3361 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     3421 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     3481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     3541 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     3601 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     3661 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     3721 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     3781 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     3841 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     3901 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     3961 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     4021 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     4081 tacaagtccg gactcagatc tcgaggatct ggagcaacaa acttctcact actcaaacaa
     4141 gcaggtgacg tggaggagaa tcccggcccc gggatgtctg cggagagcgg ccctgggacg
     4201 agattgagaa atctgccagt aatgggggat ggactagaaa cttcccaaat gtctacaaca
     4261 caggcccagg cccaacccca gccagccaac gcagccagca ccaacccccc gcccccagag
     4321 acctccaacc ctaacaagcc caagaggcag accaaccaac tgcaatacct gctcagagtg
     4381 gtgctcaaga cactatggaa acaccagttt gcatggcctt tccagcagcc tgtggatgcc
     4441 gtcaagctga acctccctga ttactataag atcattaaaa cgcctatgga tatgggaaca
     4501 ataaagaagc gcttggaaaa caactattac tggaatgctc aggaatgtat ccaggacttc
     4561 aacactatgt ttacaaattg ttacatctac aacaagcctg gagatgacat agtcttaatg
     4621 gcagaagctc tggaaaagct cttcttgcaa aaaataaatg agctacccac agaagaaacc
     4681 gagatcatga tagtccaggc aaaaggaaga ggacgtggga ggaaagaaac agggacagca
     4741 aaacctggcg tttccacggt accaaacaca actcaagcat cgactcctcc gcagacccag
     4801 acccctcagc cgaatcctcc tcctgtgcag gccacgcctc accccttccc tgccgtcacc
     4861 ccggacctca tcgtccagac ccctgtcatg acagtggtgc ctccccagcc actgcagacg
     4921 cccccgccag tgccccccca gccacaaccc ccacccgctc cagctcccca gcccgtacag
     4981 agccacccac ccatcatcgc ggccacccca cagcctgtga agacaaagaa gggagtgaag
     5041 aggaaagcag acaccaccac ccccaccacc attgacccca ttcacgagcc accctcgctg
     5101 cccccggagc ccaagaccac caagctgggc cagcggcggg agagcagccg gcctgtgaaa
     5161 cctccaaaga aggacgtgcc cgactctcag cagcacccag caccagagaa gagcagcaag
     5221 gtctcggagc agctcaagtg ctgcagcggc atcctcaagg agatgtttgc caagaagcac
     5281 gccgcctacg cctggccctt ctacaagcct gtggacgtgg aggcactggg cctacacgac
     5341 tactgtgaca tcatcaagca ccccatggac atgagcacaa tcaagtctaa actggaggcc
     5401 cgtgagtacc gtgatgctca ggagtttggt gctgacgtcc gattgatgtt ctccaactgc
     5461 tataagtaca accctcctga ccatgaggtg gtggccatgg cccgcaagct ccaggatgtg
     5521 ttcgaaatgc gctttgccaa gatgccggac gagcctgagg agccagtggt ggccgtgtcc
     5581 tccccggcag tgccccctcc caccaaggtt gtggccccgc cctcatccag cgacagcagc
     5641 agcgatagct cctcggacag tgacagttcg actgatgact ctgaggagga gcgagcccag
     5701 cggctggctg agctccagga gcagctcaaa gccgtgcacg agcagcttgc agccctctct
     5761 cagccccagc agaacaaacc aaagaaaaag gagaaagaca agaaggaaaa gaaaaaagaa
     5821 aagcacaaaa ggaaagagga agtggaagag aataaaaaaa gcaaagccaa ggaacctcct
     5881 cctaaaaaga cgaagaaaaa taatagcagc aacagcaatg tgagcaagaa ggagccagcg
     5941 cccatgaaga gcaagccccc tcccacgtat gagtcggagg aagaggacaa gtgcaagcct
     6001 atgtcctatg aggagaagcg gcagctcagc ttggacatca acaagctccc cggcgagaag
     6061 ctgggccgcg tggtgcacat catccagtca cgggagccct ccctgaagaa ttccaacccc
     6121 gacgagattg aaatcgactt tgagaccctg aagccgtcca cactgcgtga gctggagcgc
     6181 tatgtcacct cctgtttgcg gaagaaaagg aaacctcaag ctgagaaagt tgatgtgatt
     6241 gccggctcct ccaagatgaa gggcttctcg tcctcagagt cggagagctc cagtgagtcc
     6301 agctcctctg acagcgaaga ctccgaaaca ggtcctgcct aagggatcca ccggatctag
     6361 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6421 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6481 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6541 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6601 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6661 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6721 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6781 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6841 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6901 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6961 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7021 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7081 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7141 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     7201 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     7261 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7321 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     7381 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     7441 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     7501 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     7561 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     7621 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7681 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7741 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7801 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7861 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     7921 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7981 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8101 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9061 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9121 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9181 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9241 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9301 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9361 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9421 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9481 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9541 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9601 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9661 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9721 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9781 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9841 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9901 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9961 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10021 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10081 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    10141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10201 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10261 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10321 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10381 aatatgtact acaaacttag tagt
//
