LOCUS       sequence-388193-        3394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mkate2 fluorescent reporter gene under control of promoter ProD. 
            Used as a control plasmid in reporter gene assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Bernauw AJ, Crabbe V, Ryssegem F, Willaert R, Bervoets I, Peeters E
  TITLE     Molecular mechanisms of regulation by a beta-alanine-responsive 
            Lrp-type transcription factor from Acidianus hospitalis.
  JOURNAL   Microbiologyopen. 2023 Jun;12(3):e1356. doi: 10.1002/mbo3.1356.
  PUBMED    37379425
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiologyopen."; date: "2023-06"; volume: "12(3):e1356. doi"; 
            pages: " 10.1002/mbo3.1356"
COMMENT     Sequence Label: pPRC6-ProD
FEATURES             Location/Qualifiers
     source          1..3394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..52)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     643..662
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             1384..1392
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2908..2927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3316..3394,1..716))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
       61 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
      121 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
      181 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
      241 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
      301 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
      361 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
      421 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
      481 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
      541 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
      601 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
      661 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
      721 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc
      781 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
      841 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
      901 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
      961 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc
     1021 cgcttccttt agcagccctt gcgccctgag tgcttgcggc agcgtggggg atcttgaagg
     1081 gatccaaaaa aaaaccccgc cgaagcgggg tttttttttg acgatggtag tcagctgcct
     1141 actaagctgt ctagacacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg
     1201 agtggttgct ggataacttt acgggcatgc ataaggctcg tataatatat tcagggagac
     1261 cacaacggtt tccctctaca aataattttg tttaactttt actagagtca cacaggaaag
     1321 tactagatgg ttagcgagct gatcaaagaa aacatgcaca tgaaactgta tgatatcatc
     1381 gcctaaggag gttaagcata tggttagcga gctgatcaaa gaaaacatgc acatgaaact
     1441 gtatatggaa ggcaccgtga ataaccacca ctttaaatgt accagcgaag gtgaaggtaa
     1501 accgtatgaa ggcacccaga ccatgcgtat taaagcagtt gaaggtggtc cgctgccgtt
     1561 tgcatttgat attctggcaa ccagctttat gtatggcagc aaaaccttta ttaaccatac
     1621 ccagggtatc ccggattttt tcaaacagag ctttccggaa ggttttacct gggaacgtgt
     1681 taccacctat gaagatggtg gtgttctgac cgcaacccag gataccagtc tgcaggatgg
     1741 ttgtctgatt tataatgtga aaattcgcgg tgtgaacttt ccgagcaatg gtccggttat
     1801 gcagaaaaaa accctgggtt gggaagcaag caccgaaacc ctgtatccgg cagatggtgg
     1861 tctggaaggt cgtgcagata tggcactgaa actggttggt ggtggtcatc tgatttgcaa
     1921 tctgaaaacc acctatcgta gcaaaaaacc ggcaaaaaat ctgaaaatgc ctggcgtgta
     1981 ttatgttgat cgtcgtctgg aacgtattaa agaggcagat aaagaaacct atgtggaaca
     2041 gcatgaagtt gcagttgcac gttattgtga tctgccgagc aaactgggtc accgctgata
     2101 accatgggct agcggtttga agggtattgg tcggtcagtt tcacctgatt tacgtaaaaa
     2161 cccgcttcgg cgggtttttg cttttggagg ggcagaaaga tgaatgactg tccttttatt
     2221 ggagaggtgg acaagtggca tcagagttca ctcttaattc tgaacatacc cgtctttttc
     2281 gcctctttta cgtgattaac tccagcgctg ggcggccgct tactagaacg gtaccgataa
     2341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3121 gtgagagagc gcttttgaag ctggggtggg cgaagaactc cagcatgaga tccccgcgct
     3181 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     3241 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     3301 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     3361 atcgggagcg gcgataccgt aaagcacgag gaag
//
