LOCUS       sequence-388205-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Contactin/F3 scaffold 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: F3/pRC-CMV
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        79..408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      259..394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             475..1269
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(529..548)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1139..1158
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1443..1577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1480..1499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1534..1553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1608..1624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1608..1624)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1621..1643)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1632..1648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1656..1662,1663..1680,1681..1686))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1701..1722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1839..1856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2010..2598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2090..2109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2769..3560,3561..3629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3392..3411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3630..3734)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3809..3828)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4000..4379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4380..4583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4534..4554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4628..4647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4628..4646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7933..7951)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7934..7951)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7971..7988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7977..8201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8247..8709,1..51)
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8334..8353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8544..8565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg tgggtaccga
       61 gctcgaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
      361 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
      421 gcttggatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      481 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      541 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      601 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      661 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      721 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      781 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      841 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      901 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      961 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1021 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1081 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1141 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1201 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1261 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     1321 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1381 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1441 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1501 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1561 cttatcatgt ctggatccgt cgacctcgag agcttggcgt aatcatggtc atagctgttt
     1621 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1681 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1741 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1801 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1861 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2821 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2881 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2941 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3001 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3061 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3121 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3181 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3241 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3301 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3361 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3421 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3481 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3541 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3601 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3661 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3721 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     3781 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3841 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     3901 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     3961 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     4021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     4201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     4261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     4321 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     4381 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     4441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4501 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4561 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     4621 tcgaaattaa tacgactcac tatagggaga cccaagcttt cattactttt ggaaactgac
     4681 ccatctattc aacaaaagat gaaaatgcca ttgctggtca gccatctcct gctaatctcc
     4741 ttaacatctt gtctaggaga ctttacctgg cacagaagat atggtcatgg agtgtctgag
     4801 gaggacaaag gatttgggcc catttttgaa gagcagccaa tcaataccat ttacccagaa
     4861 gagtcactgg aaggaaaagt gtcactcaac tgccgggcac gcgccagccc atttccagtt
     4921 tacaaatgga gaatgaataa cggggacgtg gacctgacaa acgatcggta cagtatggtc
     4981 ggaggaaacc ttgttatcaa caacccggac aaacagaaag acgctggggt gtactactgc
     5041 ctggcatcca acaactacgg aatggtcagg agcacggagg caactctgag cttcgggtat
     5101 ctcgacccct ttcctcccga ggagcgtccg gaagtgaagg tgaaggaagg gaagggcatg
     5161 gtgcttctct gtgatcctcc ctaccacttc ccagatgatc ttagctaccg ctggcttctg
     5221 aatgaatttc ctgtatttat caccatggat aagcgaagat ttgtgtctca gaccaacggc
     5281 aatctctaca tcgcaaatgt cgagtcttct gacagaggca actactcctg ctttgtgtcc
     5341 agtccttcta ttacaaagag tgtgttcagc aagtttattc ccctcattcc gattcctgaa
     5401 cgaacaacaa agccatatcc tgctgatatt gtggtgcagt tcaaggacat atacaccatg
     5461 atgggccaaa atgtgacttt agagtgtttc gctctaggga accctgttcc tgatatccgg
     5521 tggcggaagg tgctagaacc aatgcccagc actgctgaga taagcacgtc aggggctgtc
     5581 ctcaagatct tcaacatcca gctagaagat gaaggcctct acgagtgtga ggctgagaac
     5641 atccggggaa aggacaagca ccaggcaaga atctatgttc aagcgtttcc tgagtgggta
     5701 gagcatatca atgacactga ggtggacata ggcagtgacc tctactggcc ttgcatagcc
     5761 acagggaagc ccattcctac catcaggtgg ctgaaaaatg gatactcgta tcacaaaggg
     5821 gaactgagac tgtatgatgt gacttttgaa aatgccggga tgtaccagtg catagcagaa
     5881 aacgcgtatg ggtccattta tgccaacgct gagctcaaga tcctggcttt agctccaact
     5941 tttgaaatga atcctatgaa gaaaaagatc ttggctgcga aaggtggaag ggttatcatt
     6001 gaatgtaaac ccaaggccgc accaaaaccc aagttttcat ggagcaaagg gacggagtgg
     6061 ctcgttaaca gcagcagaat actcatatgg gaagatggta gcttggaaat caataatatt
     6121 acaagaaatg atggtggaat ctatacatgc tttgcagaaa ataacagagg aaaagctaat
     6181 agcactggga ctctggtgat cacaaatccc acacgaatta ttttggcccc aatcaatgct
     6241 gacatcactg ttggggaaaa tgccaccatg cagtgtgctg cgtctttcga tcctgccttg
     6301 gacctcacgt ttgtgtggtc cttcaatggc tacgtgattg actttaacaa ggaaattacg
     6361 catatccatt accagaggaa ttttatgctc gacgccaatg gggaactgct catccgaaat
     6421 gcgcagctga aacacgcagg ccggtacacg tgcacggcgc agacaatcgt ggacaattcc
     6481 tcggcttccg ctgaccttgt cgttcgaggc cccccaggtc ccccaggagg cctgagaata
     6541 gaagacatca gagctacctc tgtggcactg acttggagcc gtggttccga caatcacagc
     6601 cctatctcta aatacacgat ccagaccaag acgattctct cagatgactg gaaagatgcg
     6661 aagacagatc ccccaatcat tgaaggaaac atggagtcag caaaagcagt ggatttgatc
     6721 ccttggatgg agtatgaatt tcgagtggtg gcgaccaata ctttagggac aggagagccc
     6781 agcataccct caaacaggat caaaacagac ggtgctgcac caaatgtggc tccctcagat
     6841 gtaggcggtg ggggcggaac caacagagag ctgacaataa cgtgggcgcc tttgtcgaga
     6901 gagtaccatt acggtaacaa ttttggttac atagtggcgt ttaagccatt tgatggagaa
     6961 gagtggaaga aagtgactgt gactaaccca gacactggcc gctacgtcca caaagatgag
     7021 accatgaccc cctccaccgc gtttcaagtc aaagtgaaag ccttcaacaa taaaggagat
     7081 gggccctaca gcctcgtagc tgtcatcaac tctgcacaag atgctcccag tgaagccccc
     7141 acagaggtag gggtcaaagt gttatcatcc tctgagatat ctgtccattg gaagcatgtg
     7201 ttagagaaaa ttgtggaaag ctatcagatt cggtactggg ctgggcatga caaagaagca
     7261 gccgcacaca gagttcaggt caccagccag gagtactcag ccagactgga gaaccttctt
     7321 cccgacaccc agtacttcat tgaagtggga gcctgcaaca gtgccggatg cggaccttcc
     7381 agtgatgtga tcgagacctt caccagaaaa gcccctccta gccaaccacc aaggatcatc
     7441 agttcggtga gatctggctc acgatatata atcacatggg atcacgttgt ggcactatcc
     7501 aatgaatcta ctgtgacagg ctacaagata ctctatagac cggatggcca acatgacggc
     7561 aagctgttct caacccataa acactccata gaagtcccca tccccagaga cggagagtat
     7621 gttgtcgagg ttcgagcaca cagcgacggg ggagacggag tcgtgtctca agtcaaaatt
     7681 tcaggcgtgt ccacgctgtc ttcgagtctc ctcagcttgc tcctgccctc ccttggcttt
     7741 cttgtctact cggaattctg aatgttacga ccttggctgt tttcatccct gctcagagga
     7801 tgctcttcat catgggatga ctgtaattcc ttctaatccc tgcggctcca tcctaagcca
     7861 aacaaattag actctaacaa gctgttcaac tgatttccca acacatcacg actgaggcat
     7921 tcaggaacct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     7981 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     8041 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8101 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8161 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8221 cagctggggc tcgagggggg atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8641 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8701 ttaaatatt
//
