LOCUS       sequence-388234-        5943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / 
            TAOK1 (aa1-416, K57A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Tavares IA, Touma D, Lynham S, Troakes C, Schober M, Causevic M, 
            Garg R, Noble W, Killick R, Bodi I, Hanger DP, Morris JD
  TITLE     Prostate-derived sterile 20-like kinases (PSKs/TAOKs) phosphorylate 
            tau protein and are activated in tangle-bearing neurons in Alzheimer
            disease.
  JOURNAL   J Biol Chem. 2013 May 24;288(21):15418-29. doi: 
            10.1074/jbc.M112.448183. Epub 2013 Apr 12.
  PUBMED    23585562
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.448183"; journalName: "J Biol Chem"; date: 
            "2013-05-24- 24"; volume: "288"; issue: "21"; pages: "15418-29"
COMMENT     Sequence Label: pRK5-Myc-PSK2 (1-416) (K57A)
FEATURES             Location/Qualifiers
     source          1..5943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2243..2252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2256..2390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2293..2312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2347..2366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2459..2816
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2459..2650
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2482..2502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2667..2802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2836..2853)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2836..2852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2845..2867)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3065..3520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3152..3171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3362..3383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3537..3556)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3656..3678
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3716..3734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3802..3906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3907..3975,3976..4767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4125..4144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4938..5526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5427..5446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5680..5697
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5814..5835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5850..5855,5856..5873,5874..5880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5888..5904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5893..5915
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5912..5928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5912..5928
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attggccacc atggagcaga agctgatctc cgaggaggac
      961 ctgggatcca tgccatcaac taacagagca ggcagcctga aggaccctga aattgcagag
     1021 ctcttcttca aagaagatcc agagaagctc ttcacagatc tcagagaaat tggccatgga
     1081 agctttggag cagtgtattt tgcacgagat gtgcgtacca atgaagtggt ggccatcgcg
     1141 aaaatgtctt atagtggaaa gcagtctact gagaaatggc aggatattat taaggaagtc
     1201 aagtttctac aaagaataaa acatcccaac agtatagaat acaaaggctg ttatttacgt
     1261 gaacacacag catggcttgt aatggaatat tgtttaggat ctgcttcgga tttactagaa
     1321 gttcacaaaa agccattaca agaagtggaa atagcagcaa ttacacatgg tgctcttcag
     1381 ggattagcct acttacattc tcatactatg attcatagag atatcaaagc aggaaatatc
     1441 cttctgacag aaccaggcca ggtgaaactt gctgactttg gctctgcttc catggcatca
     1501 cctgccaatt cctttgtggg aacgccgtat tggatggccc cagaagtaat tttagccatg
     1561 gatgaaggac aatatgatgg caaagtagat gtgtggtctc ttggaataac atgtattgaa
     1621 ctagcggaaa ggaagcctcc tttatttaat atgaatgcaa tgagtgcctt atatcacata
     1681 gcccaaaatg aatcccctac actacagtct aatgaatggt ctgattattt tcgcaacttt
     1741 gtagattctt gcctccagaa aatccctcaa gatcgaccta catcagagga acttttaaag
     1801 cacatatttg ttcttcggga gcgccctgaa accgtgttaa tagatctcat tcagaggaca
     1861 aaggatgcag taagagagct ggacaatctg cagtatcgaa agatgaagaa actccttttc
     1921 caggaggcac ataatggacc agcagtagaa gcacaggaag aagaagagga acaagatcat
     1981 ggtgttggcc ggacaggaac agttaatagt gttggaagta atcaatccat tcccagcatg
     2041 tccatcagtg ccagcagcca aagcagtagt gttaacagtc ttccagatgt ctcagatgac
     2101 aagagtgagc tagacatgat ggagggagac cacacagtga tgtctaacag ttctgttatc
     2161 catttaaaac cagaggaaga aaattacaga gaagagggag atcctagaac aagagcatca
     2221 tgagaattcc tgcagaagct tggccgccat ggcccaactt gtttattgca gcttataatg
     2281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat
     2401 tcggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     2461 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2521 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     2581 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2641 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     2761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     2821 tgttaacagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2881 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2941 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     3001 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3061 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     3421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3481 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     3541 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     3601 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3661 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3721 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3781 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3841 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3901 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3961 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4021 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4081 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4141 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4201 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4261 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4321 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4381 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4441 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4501 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4561 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4621 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4681 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4741 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4801 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4861 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4921 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4981 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5041 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5101 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5161 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5221 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5281 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5341 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5401 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5461 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5521 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5581 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5641 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5701 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5761 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5821 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5881 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     5941 taa
//
