LOCUS       sequence-388238-       10686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP-tagged TAOK2 / PSK1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Garg R, Koo CY, Infante E, Giacomini C, Ridley AJ, Morris JDH
  TITLE     Rnd3 interacts with TAO kinases and contributes to mitotic cell 
            rounding and spindle positioning.
  JOURNAL   J Cell Sci. 2020 Mar 16;133(6):jcs235895. doi: 10.1242/jcs.235895.
  PUBMED    32041905
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-03-16"; volume: "133(6):jcs235895. doi"; pages: "
            10.1242/jcs.235895"
COMMENT     Sequence Label: CB6-GFP-TAOK2
FEATURES             Location/Qualifiers
     source          1..10686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(944..946,947..1657)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKGDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(986..1007)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1594..1615
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1668..1684
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1669..1685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5421..5437)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5421..5437)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    5445..6067
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5644..5663)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6091..6111)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6109..6406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6460..7254
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6514..6533)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7124..7143
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7906..8040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7943..7962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7997..8016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8045..8064)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8046..8064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8078..8094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8078..8094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8091..8113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8102..8118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8126..8132,8133..8150,8151..8156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8171..8192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8309..8326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8480..9068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8560..8579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9239..10030,10031..10099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9862..9881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10100..10204)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10231..10659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10318..10337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10528..10549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcag atctggtacc accatgagca agggcgagga
      961 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1021 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1081 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1141 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1201 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaagggcg acggcaacta
     1261 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1321 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1381 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1441 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1501 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1561 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1621 cgccgggatc actctcggca tggacgagct gtacaagggc ggccgcctcg aggtcgacgg
     1681 tatcgataag cttatgccag ctgggggccg ggccgggagc ctgaaggacc cagatgtggc
     1741 tgagctcttc ttcaaggatg acccagaaaa gctcttctct gacctccggg aaattggcca
     1801 tggcagcttt ggagccgtat actttgcccg ggatgtccgg aatagtgagg tggtggccat
     1861 caagaagatg tcctacagtg ggaagcagtc caatgagaaa tggcaagaca tcatcaagga
     1921 ggtgcggttc ttacagaagc tccggcatcc caacaccatt cagtaccggg gctgttacct
     1981 gagggagcac acggcttggc tggtaatgga gtattgcctg ggctcagctt ctgaccttct
     2041 agaagtgcac aagaaacccc ttcaggaggt agagatcgca gctgtgaccc acggggcgct
     2101 tcagggcctg gcatatctgc actcccacaa catgatccat agggatgtga aggctggaaa
     2161 catcctgctg tcagagccag ggttagtgaa gctaggggac tttggttctg cgtccatcat
     2221 ggcacctgcc aactccttcg tgggcacccc atactggatg gcacccgagg tgatcctggc
     2281 catggatgag gggcagtacg atggcaaagt ggacgtctgg tccttgggga taacctgcat
     2341 cgagctggct gaacggaaac caccgctctt taacatgaat gcgatgagtg ccttatacca
     2401 cattgcacag aacgaatccc ccgtgctcca gtcaggacac tggtctgagt acttccggaa
     2461 ttttgtcgac tcctgtcttc agaaaatccc tcaagacaga ccaacctcag aggttctcct
     2521 gaagcaccgc tttgtgctcc gggagcggcc acccacagtc atcatggacc tgatccagag
     2581 gaccaaggat gccgtgcggg agctggacaa cctgcagtac cgcaagatga agaagatcct
     2641 gttccaagag gcacccaacg gccctggtgc cgaggcccca gaggaggaag aggaggccga
     2701 gccctacatg caccgggccg ggactctgac cagcctcgag agtagccact cagtgcccag
     2761 catgtccatc agcgcctcca gccagagcag ctccgtcaac agcctagcag atgcctcaga
     2821 caacgaggaa gaggaggagg aggaggagga agaggaggag gaggaagaag gccctgaagc
     2881 ccgggagatg gccatgatgc aggaggggga gcacacagtc acctctcaca gctccattat
     2941 ccaccggctg ccgggctctg acaacctata tgatgacccc taccagccag agataacccc
     3001 cagccctctc cagccgcctg cagccccagc tcccacttcc accacctctt ccgcccgccg
     3061 ccgggcctac tgccgtaacc gagaccactt tgccaccatc cgaaccgcct ccctggtcag
     3121 ccgtcagatc caggagcatg agcaggactc tgcgctgcgg gagcagctga gcggctataa
     3181 gcggatgcga cgacagcacc agaagcagct gctggccctg gagtcacggc tgaggggtga
     3241 acgggaggag cacagtgcac ggctgcagcg ggagcttgag gcgcagcggg ctggctttgg
     3301 ggcagaggca gaaaagctgg cccggcggca ccaggccata ggtgagaagg aggcacgagc
     3361 tgcccaggcc gaggagcgga agttccagca gcacatcctt gggcagcaga agaaggagct
     3421 ggctgccctg ctggaggcac agaagcggac ctacaaactt cgcaaggaac agctgaagga
     3481 ggagctccag gagaacccca gcactcccaa gcgggagaag gccgagtggc tgctgcggca
     3541 gaaggagcag ctccagcagt gccaggcgga ggaggaagca gggctgctgc ggcggcagcg
     3601 ccagtacttt gagctgcagt gtcgccagta caagcgcaag atgttgctgg ctcggcacag
     3661 cctggaccag gacctgctgc gggaggacct gaacaagaag cagacccaga aggacttgga
     3721 gtgtgcactg ctgcttcggc agcacgaggc cacgcgggag ctggagctgc ggcagctcca
     3781 ggccgtgcag cgcacgcggg ctgagctcac ccgcctgcag caccagacgg agctgggcaa
     3841 ccagctggag tacaacaagc ggcgtgagca agagttgcgg cagaagcatg cggcccaggt
     3901 tcgccagcag cccaagagcc tcaaagtacg tgcaggccag cgccccccgg gccttccact
     3961 ccccattcct ggggctctgg gcccacccaa cacaggcacc cctatagaac agcagccctg
     4021 ctcacctggc caggaggcag tcctggacca aagaatgctt ggcgaggagg aggaagcagt
     4081 tggagagaga aggattctgg gaaaggaagg ggccactttg gagcccaagc agcagaggat
     4141 tctgggggaa gaatcaggag cccctagtcc cagtccacaa aaacatggga gcctggttga
     4201 tgaggaagtt tggggtctgc ctgaggagat agaggagctt agggtgccct cccttgtacc
     4261 ccaggagagg agcattgttg gccaggagga ggctgggacg tggagcttgt gggggaagga
     4321 ggatgagagt cttctggatg aggagtttga gcttggctgg gtccagggcc cagcactgac
     4381 tcccgtccct gaggaggagg aagaagagga agagggggct ccgattggga cccctaggga
     4441 tcctggagat ggttgtcctt cccccgacat ccctcctgaa ccccctccaa cacacctgag
     4501 gccctgccct gccagccagc tccctggact cctgtcccat ggcctcctgg ccggcctctc
     4561 ctttgcagtg gggtcctcct ctggcctcct gcccctcctg ctgctgctgc tgcttccatt
     4621 gctggcagcc cagggtgggg gtggcctgca ggcagcgctg ctggcccttg aggtggggct
     4681 ggtgggtctg ggggcctcct acctgctcct ttgtacagcc ctgcacctgc cctccagtct
     4741 tttcctactc ctggcccagg gtaccgcact gggggccgtc ctgggcctga gctggcgccg
     4801 aggcctcatg ggtgttcccc tgggccttgg agctgcctgg ctcttagctt ggccaggcct
     4861 agctctacct ctggtggcta tggcagcggg gggcagatgg gtgcggcagc agggcccccg
     4921 ggtgcgccgg ggcatatctc gactctggtt gcgggttctg ctgcgcctgt cacccatggc
     4981 cttccgggcc ctgcagggct gtggggctgt gggggaccgg ggtctgtttg cactgtaccc
     5041 caaaaccaac aaggatggct tccgcagccg cctgcccgtc cctgggcccc ggcggcgtaa
     5101 tccccgcacc acccaacacc cattagctct gttggcaagg gtctgggtcc tgtgcaaggg
     5161 ctggaactgg cgtctggcac gggccagcca gggtttagca tcccacttgc ccccgtgggc
     5221 catccacaca ctggccagct ggggcctgct tcggggtgaa cggcccaccc gaatcccccg
     5281 gctactacca cgcagccagc gccagctagg gccccctgcc tcccaccagc cactgccagg
     5341 gactctagcc gggcggaggt cacgcacccg ccagtcccgg gccctgcccc cctggaggta
     5401 ggaattcctg cagcccgggg gatccactag ttctagagga tcccgggtgg catccctgtg
     5461 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     5521 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     5581 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     5641 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     5701 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     5761 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     5821 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     5881 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat
     5941 accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac
     6001 atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg
     6061 ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggagga attgatcagc
     6421 ttgggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     6481 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6541 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6601 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6661 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6721 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6781 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6841 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6901 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6961 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7021 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7081 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7141 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7201 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7261 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7321 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7381 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc
     7441 tcgcgagttg gttcagctgc tgcctgaggc tggacgacct cgcggagttc taccggcagt
     7501 gcaaatccgt cggcatccag gaaaccagca gcggctatcc gcgcatccat gcccccgaac
     7561 tgcaggagtg gggaggcacg atggccgctt tggtcgaccc ggacgggacg ctcctgcgcc
     7621 tgatacagaa cgaattgctt gcaggcatct catgagtgtg tcttcccgtt ttccgcctga
     7681 ggtcactgcg tggatggagc gctggcgcct gctgcgcgac ggcgagctgc tcaccaccca
     7741 ctcgccaagc tggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccg
     7801 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     7861 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     7921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     8041 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
     8101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10201 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
    10261 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    10321 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    10381 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    10441 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    10501 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    10561 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    10621 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    10681 caattt
//
