LOCUS       sequence-388240-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA 
            synthetase/Pyl-tRNA pair for the encoding of fluorinated 
            phenylalanine derivatives at TAG codons in HEK293 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Galles GD, Infield DT, Clark CJ, Hemshorn ML, Manikandan S, Fazan F,
            Rasouli A, Tajkhorshid E, Galpin JD, Cooley RB, Mehl RA, Ahern CA
  TITLE     Tuning phenylalanine fluorination to assess aromatic contributions 
            to protein function and stability in cells.
  JOURNAL   Nat Commun. 2023 Jan 4;14(1):59. doi: 10.1038/s41467-022-35761-w.
  PUBMED    36599844
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-4"; volume: "14(1):59. doi"; pages: " 
            10.1038/s41467-022-35761-w"
COMMENT     Sequence Label: pAcBac1-Mb-Fluoro-Phe D6
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  83..248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(341..435)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    341..359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(512..760)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    793..927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(817..836)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     871..890
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1073..2560,2561..2608))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     promoter        complement(2667..2758)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2725..2742)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     enhancer        2853..3232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3233..3436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3387..3407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3481..3500
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3481..3499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3519..3528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3549..3575
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4885..5473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4938..4958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5356..5367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5522..5539)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5528..5752
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5789..6037
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        6114..6208
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    6162..6180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6190..6208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(6698..6703,6704..6720,6721..6726)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6915..7448
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  7515..7739
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(7873..7890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8044..8632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8124..8143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8803..9594,9595..9663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9426..9445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9664..9768)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9794..10248)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9931..9952)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10142..10161
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat
       61 aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg
      121 aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta
      181 gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt
      241 atctggttcg gatctcctag caaaaaacgg ggggtgcggg agtttcaccc gcctgcacgg
      301 atttagagtc cgtgtgatct attcgatcca ccccccggtg tctctatcac tgatagggaa
      361 cttataagtc tctatcactg atagggattt cacgtttatg gtgatttccc agaacacata
      421 gcgacatgca aatattaaaa aacggaaacc ccgggaatct aacccggctg aacggattta
      481 gagtccgttc gatctacatg atcaggtttc cggtgtttcg tcctttccac aagatatata
      541 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      601 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      661 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      721 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg acctaggctc
      781 aagcagtgat cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
      841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      961 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1021 gctgattatg atcctctagt acttctcgac aagcttggcc ggcctggcct tatcactttc
     1081 caagtcggtt catctctatg tctgtataaa tctgtctttt cttggtgtgc tttaatttaa
     1141 tgcaaagatg gataccaact cggagaacca agaatagtcc aatgattaac cctatgataa
     1201 agaaaaaaga ggcaatagag cttttccaac tactgaacca accttctaca agctcgattg
     1261 gatttttgga tagcccagta tcaccaaaaa ataaactctc atcatcagga agttgcgaag
     1321 cagcgtcttg aatgtgagga tgttcgaaca cctgagcctt tgagctaaga tgaagatcgg
     1381 agtccaacat accatgtcca atcatgtata aaggaaactt atatcctgaa ctggtcctca
     1441 gaactccatt gggtccaatt tccacgtctt catatggtgc ccagtcatcc cacagttccc
     1501 tttctgtggt agttccactg atcattccga ccattcttga gaggattgga gcagcaatat
     1561 cgactctgat gtatctggtc tcaaagtatt ttagggtacc attgattatg gtgaaagcag
     1621 gaccggttcc tgggttttta ggagcaagat agctgagatc cactggagag attggaagac
     1681 ccgctctgat tttgctccag gtttcttggc agagggaata atccaagatc ctctcaacgt
     1741 cctgaattag acttacatcc actgaggtct gagatggagc agagatactt gacccttctg
     1801 ggcattcagg gaatctggct gcagcaaaga gatccttatc agccatctcg aaccagacac
     1861 ctgatgggag tctgactccc caatgcttgc agtattgcat tttgcaggcc ttgcctccag
     1921 tttcataagc aaagtagtta cttctgaacc ctgtgccctc ctttcccagg gatgatagct
     1981 ctccgtcctc tgagaagaag gtgatgtcca tggaaatgag gttagaatca catagccctt
     2041 tgaccttata gtcagaatgc caggttgtag agttatggac agtggggcat atgtaattgc
     2101 tgcattttcc gttgatgaac tgtgaatcaa cccattctcc tgtgtattca tcaaccagca
     2161 catggtgagg agtcacctgg acaatcactg cttcggcatc cgtcacagtt gcatatccac
     2221 aactttgagg agggaagcct ggattcagcc aagttccttg tttcgtttgt tcaatgcttt
     2281 ccttgcattg ttctacagat ggagtgaagg atcggatgga atgtgttata tacttcggtc
     2341 cataccagcg gaaatcacaa gtagtgaccc atttggaagc atgacacatc caaccgtctg
     2401 cttgaatagc cttgtgactc ttgggcattt tgacttgtaa ggctgtgcct attaagtcat
     2461 tatgccaatt taaatctgag cttgacgggc aataatggta attagaagga acatttttcc
     2521 agtttccttt ttggttgtgt ggaaaaacta tggtgaactt gcaattcacc ccaatgaata
     2581 aaaaggctaa gtacaaaagg cacttcatgg tggcggtttt ttaggagttc gcgcccgatg
     2641 gtgggacggt atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga
     2701 aaacagtaaa atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct
     2761 attaatattc cggagtatac ggaccgcggc cgcaaatacc tgcaggatcc gttttgcgct
     2821 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     2881 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2941 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3001 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3061 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3121 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3181 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3241 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3301 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3361 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3421 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     3481 taatacgact cactataggg agacccaagc tggctagcgc caccatggcg tgtccggttc
     3541 ctttgcagtt gcctccactg gagcgcctca cactcgacga ctacaaggac gacgacgaca
     3601 aggacaagaa acccctggac gtgctgatca gcgccaccgg cctgtggatg agccggaccg
     3661 gcaccctgca caagatcaag caccacgagg tgtcaagaag caaaatctac atcgagatgg
     3721 cctgcggcga ccacctggtg gtgaacaaca gcagaagctg ccggaccgcc agagccttcc
     3781 ggcaccacaa gtacagaaag acctgtaagc ggtgccgggt gtccgacgag gacatcaaca
     3841 actttctgac cagaagcacc gagagcaaga acagcgtgaa agtgcgggtg gtgtccgccc
     3901 ccaaagtgaa gaaagccatg cccaagagcg tgtccagagc ccccaagccc ctggaaaaca
     3961 gcgtgtccgc caaggccagc accaacacca gccgcagcgt gcccagcccc gccaagagca
     4021 cccccaacag ctccgtgccc gcctctgctc ctgctcccag cctgacacgg tcccagctgg
     4081 acagagtgga ggccctgctg tcccccgagg acaagatcag cctgaacatg gccaagccct
     4141 tccgggagct ggaacccgag ctggtgaccc ggcggaagaa cgacttccag cggctgtaca
     4201 ccaacgaccg ggaggactac ctgggcaagc tggaacggga catcaccaag ttcttcgtgg
     4261 accggggctt cctggaaatc aagagcccca tcctgatccc cgccgagtac gtggagcgga
     4321 tgggcatcaa caacgacacc gagctgtcca agcagatttt ccgggtggac aagaacctgt
     4381 gcctgcggcc tatgctggcc cccaccctgt acaactacct gcggaaactg gacagaatcc
     4441 tgcctggccc catcaagatt ttcgaagtgg gaccctgcta ccggaaagag agcgacggca
     4501 aagagcacct ggaagagttt acaatggtgg cctttatgca gatgggcagc ggctgcaccc
     4561 gggagaacct ggaagccctg atcaaagagt tcctggatta cctggaaatc gacttcgaga
     4621 tcgtgggcga cagctgcatg gtgtacggcg acaccctgga catcatgcac ggcgacctgg
     4681 aactgagcag cgccggcgtg ggacccgtgt ccctggaccg ggagtggggc atcgacaagc
     4741 ccaccatcgg agccggcttc ggcctggaac ggctgctgaa agtgatgcac ggcttcaaga
     4801 acatcaagcg ggccagcaga agcgagagct actacaacgg catcagcacc aacctgtgat
     4861 gagaattcaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4921 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4981 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5041 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5101 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5161 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5221 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5281 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5341 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5401 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5461 gccgcctccc cgcgtcgact ttaactcgag tctagagggc ccgtttaaac ccgctgatca
     5521 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5581 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5641 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5701 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5761 gcggaaagaa ccagatctcg ggcaggaaga gggcctattt cccatgattc cttcatattt
     5821 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     5881 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     5941 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     6001 cttggcttta tatatcttgt ggaaaggacg aaacaccgga aacctgatca tgtagatcga
     6061 acggactcta aatccgttca gccgggttag attcccgggg tttccgtttt ttaatatttg
     6121 catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga
     6181 cttataagtt ccctatcagt gatagagaca ccggggggtg gatcgaatag atcacacgga
     6241 ctctaaatcc gtgcaggcgg gtgaaactcc cgcacccccc gttttttcag ctggggctct
     6301 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg caagccctgc catagccact
     6361 acgggtacgt agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc
     6421 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg
     6481 ccgcgacggc cgaggtctac cgatctcctg aagcaagggc agatccgtgc acagcacctt
     6541 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg
     6601 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     6661 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
     6721 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     6781 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     6841 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     6901 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     6961 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     7021 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
     7081 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     7141 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     7201 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
     7261 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     7321 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     7381 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     7441 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
     7501 tgtcacaacg ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg
     7561 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta
     7621 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga
     7681 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac
     7741 ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg
     7801 ctggtctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8221 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8461 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9781 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     9841 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     9901 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaga
     9961 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    10021 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    10081 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    10141 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    10201 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
    10261 tcaggctgca aataagcgtt gatattcagt caattacaaa cattaataac gaagagatga
    10321 cagaaaaatt ttcattctgt gacagagaa
//
