LOCUS       sequence-388256-        8175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of MYC-tagged PSK1-beta in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Zihni C, Mitsopoulos C, Tavares IA, Baum B, Ridley AJ, Morris JD
  TITLE     Prostate-derived sterile 20-like kinase 1-alpha induces apoptosis. 
            JNK- and caspase-dependent nuclear localization is a requirement for
            membrane blebbing.
  JOURNAL   J Biol Chem. 2007 Mar 2;282(9):6484-93. doi: 10.1074/jbc.M608336200.
            Epub 2006 Dec 11.
  PUBMED    17158878
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M608336200"; journalName: "J Biol Chem"; date: 
            "2007-03-2- 2"; volume: "282"; issue: "9"; pages: "6484-93"
COMMENT     Sequence Label: pRK5-Myc-PSK1-beta
FEATURES             Location/Qualifiers
     source          1..8175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4475..4484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4488..4622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4525..4544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4579..4598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4691..5048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4691..4882
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4714..4734)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4899..5034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5068..5085)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5068..5084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5077..5099)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5297..5752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5384..5403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5594..5615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5769..5788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5888..5910
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5948..5966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6034..6138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6139..6207,6208..6999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6357..6376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7170..7758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7659..7678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7912..7929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8046..8067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8082..8087,8088..8105,8106..8112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8120..8136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8125..8147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8144..8160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8144..8160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attggccacc atggagcaga agctgatctc cgaggaggac
      961 ctgggatcca tgccagctgg gggccgggcc gggagcctga aggacccaga tgtggctgag
     1021 ctcttcttca aggatgaccc agaaaagctc ttctctgacc tccgggaaat tggccatggc
     1081 agctttggag ccgtatactt tgcccgggat gtccggaata gtgaggtggt ggccatcaag
     1141 aagatgtcct acagtgggaa gcagtccaat gagaaatggc aagacatcat caaggaggtg
     1201 cggttcttac agaagctccg gcatcccaac accattcagt accggggctg ttacctgagg
     1261 gagcacacgg cttggctggt aatggagtat tgcctgggct cagcttctga ccttctagaa
     1321 gtgcacaaga aaccccttca ggaggtagag atcgcagctg tgacccacgg ggcgcttcag
     1381 ggcctggcat atctgcactc ccacaacatg atccataggg atgtgaaggc tggaaacatc
     1441 ctgctgtcag agccagggtt agtgaagcta ggggactttg gttctgcgtc catcatggca
     1501 cctgccaact ccttcgtggg caccccatac tggatggcac ccgaggtgat cctggccatg
     1561 gatgaggggc agtacgatgg caaagtggac gtctggtcct tggggataac ctgcatcgag
     1621 ctggctgaac ggaaaccacc gctctttaac atgaatgcga tgagtgcctt ataccacatt
     1681 gcacagaacg aatcccccgt gctccagtca ggacactggt ctgagtactt ccggaatttt
     1741 gtcgactcct gtcttcagaa aatccctcaa gacagaccaa cctcagaggt tctcctgaag
     1801 caccgctttg tgctccggga gcggccaccc acagtcatca tggacctgat ccagaggacc
     1861 aaggatgccg tgcgggagct ggacaacctg cagtaccgca agatgaagaa gatcctgttc
     1921 caagaggcac ccaacggccc tggtgccgag gccccagagg aggaagagga ggccgagccc
     1981 tacatgcacc gggccgggac tctgaccagc ctcgagagta gccactcagt gcccagcatg
     2041 tccatcagcg cctccagcca gagcagctcc gtcaacagcc tagcagatgc ctcagacaac
     2101 gaggaagagg aggaggagga ggaggaagag gaggaggagg aagaaggccc tgaagcccgg
     2161 gagatggcca tgatgcagga gggggagcac acagtcacct ctcacagctc cattatccac
     2221 cggctgccgg gctctgacaa cctatatgat gacccctacc agccagagat aacccccagc
     2281 cctctccagc cgcctgcagc cccagctccc acttccacca cctcttctgc ccgccgccgg
     2341 gcctactgcc gtaaccgaga ccactttgcc accatccgaa ccgcctccct ggtcagccgt
     2401 cagatccagg agcatgagca ggactctgcg ctgcgggagc agctgagcgg ctataagcgg
     2461 atgcgacgac agcaccagaa gcagctgctg gccctggagt cacggctgag gggtgaacgg
     2521 gaggagcaca gtgcacggct gcagcgggag cttgaggcgc agcgggctgg ctttggggca
     2581 gaggcagaaa agctggcccg gcggcaccag gccataggtg agaaggaggc acgagctgcc
     2641 caggccgagg agcggaagtt ccagcagcac atccttgggc agcagaagaa ggagctggct
     2701 gccctgctgg aggcacagaa gcggacctac aaacttcgca aggaacagct gaaggaggag
     2761 ctccaggaga accccagcac tcccaagcgg gagaaggccg agtggctgct gcggcagaag
     2821 gagcagctcc agcagtgcca ggcggaggag gaagcagggc tgctgcggcg gcagcgccag
     2881 tactttgagc tgcagtgtcg ccagtacaag cgcaagatgt tgctggctcg gcacagcctg
     2941 gaccaggacc tgctgcggga ggacctgaac aagaagcaga cccagaagga cttggagtgt
     3001 gcactgctgc ttcggcagca cgaggccacg cgggagctgg agctgcggca gctccaggcc
     3061 gtgcagcgca cgcgggctga gctcacccgc ctgcagcacc agacggagct gggcaaccag
     3121 ctggagtaca acaagcggcg tgagcaagag ttgcggcaga agcatgcggc ccaggttcgc
     3181 cagcagccca agagcctcaa atctaaggag ctgcagatca agaagcagtt ccaggagacg
     3241 tgtaagatcc agactcggca gtacaaggct ctgcgagcac acttgctgga gaccacgccc
     3301 aaagctcagc acaagagcct ccttaagcgg ctcaaggaag agcagacccg caagctggcg
     3361 atcttggcgg agcagtatga ccagtccatc tcagagatgc tcagctcaca ggcgctgcgg
     3421 cttgatgaga cccaggaggc agagttccag gcccttcggc agcagcttca acaggagctg
     3481 gagctgctca acgcttacca gagcaagatc aagatccgca cagagagcca gcacgagagg
     3541 gagctgcggg agctggagca gagggtcgcg ctgcggcggg cactgctgga gcagcgggtg
     3601 gaagaggagc tgctggccct gcagacagga cgctccgagc gaatccgcag tctgcttgag
     3661 cggcaggccc gtgagatcga ggccttcgat gcggaaagca tgaggctggg cttctccagc
     3721 atggctctgg ggggcatccc ggctgaagct gctgcccagg gctatcctgc tccaccccct
     3781 gccccagcct ggccctcccg tcccgttccc cgttctgggg cacactggag ccatggccct
     3841 cctccaccag gcatgccccc tccagcctgg cgtcagccgt ctctgctggc tcccccaggc
     3901 cccccaaact ggctggggcc ccccacacaa agtgggacac cccgtggcgg agccctgctg
     3961 ctgctaagaa acagccccca gcccctgcgg cgggcagcct cggggggcag tggcagtgag
     4021 aatgtgggcc cccctgctgc cgcggtgccc gggcccctga gccgcagcac cagtgtcgct
     4081 tcccacatcc tcaatggttc ttcccacttc tattcctgag gtgcagcggg gaggagcaga
     4141 tgagctgggc agggcagggg tgggtggagc ctgaccctgg agggcactga gctggaggcc
     4201 cctgcaaggg taggggacaa gatgtaggct ccagctcccc tcagacctcc tcatctcatg
     4261 agcttcttgg ggctggccag tggcccaggg ccagcttggc gatagatgcc tcaaggctgc
     4321 ctgggagccc cgcctcccta ccatggtgcc aggggtctcc ctccgccacc taggaaagga
     4381 gggagatgtg cgtgtcaaat attcatctag tcccctgggg gaggggaagg gtgggtctag
     4441 agcattgagt agatagaatt cctgcagaag cttggccgcc atggcccaac ttgtttattg
     4501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     4621 tcgggaatta attcggcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     4681 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4741 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4801 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4861 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4921 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4981 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5041 tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     5101 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5161 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5221 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     5281 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5341 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5401 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5461 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     5521 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5581 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     5641 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5701 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     5761 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5821 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5881 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5941 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6001 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6061 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6361 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7981 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8161 atgattacga attaa
//
