LOCUS       sequence-38826-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Koivusalo M, Welch C, Hayashi H, Scott CC, Kim M, Alexander T, 
            Touret N, Hahn KM, Grinstein S
  TITLE     Amiloride inhibits macropinocytosis by lowering submembranous pH and
            preventing Rac1 and Cdc42 signaling.
  JOURNAL   J Cell Biol. 2010 Feb 22;188(4):547-63. Epub 2010 Feb 15.
  PUBMED    20156964
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-02-22"; volume: "188(4)"; pages: "547-63. Epub 
            2010 Feb 15"
COMMENT     Sequence Label: mCherry-SEpHluorin
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..782
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(220..238)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     488..507
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(825..853)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca
       61 agcttcgaat tcccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      121 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      181 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      241 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      301 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      361 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      421 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      481 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      541 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
      601 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
      661 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
      721 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
      781 agggatccgc caccatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      841 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      901 atgcaacata cggaaaactt acccttaaat t
//
