LOCUS       sequence-388268-        6213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian 
            cells (microtubule-binding domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Mitsopoulos C, Zihni C, Garg R, Ridley AJ, Morris JD
  TITLE     The prostate-derived sterile 20-like kinase (PSK) regulates 
            microtubule organization and stability.
  JOURNAL   J Biol Chem. 2003 May 16;278(20):18085-91. doi: 
            10.1074/jbc.M213064200. Epub 2003 Mar 13.
  PUBMED    12639963
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M213064200"; journalName: "J Biol Chem"; date: 
            "2003-05-16- 16"; volume: "278"; issue: "20"; pages: "18085-91"
COMMENT     Sequence Label: pRK5 PSK (745-1235)
FEATURES             Location/Qualifiers
     source          1..6213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2513..2522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2526..2660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2563..2582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2617..2636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2729..3086
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2729..2920
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2752..2772)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2937..3072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3106..3123)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3106..3122)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3115..3137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3335..3790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3422..3441)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3632..3653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3807..3826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3926..3948
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3986..4004)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4072..4176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4177..4245,4246..5037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4395..4414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5208..5796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5697..5716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5950..5967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6084..6105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6120..6125,6126..6143,6144..6150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6158..6174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6163..6185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6182..6198
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6182..6198
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attggccacc atggagcaga agctgatctc cgaggaggac
      961 ctgggatcca tggtacgtgc aggccagcgc cccccgggcc ttccactccc cattcctggg
     1021 gctctgggcc cacccaacac aggcacccct atagaacagc agccctgctc acctggccag
     1081 gaggcagtcc tggaccaaag aatgcttggc gaggaggagg aagcagttgg agagagaagg
     1141 attctgggaa aggaaggggc cactttggag cccaagcagc agaggattct gggggaagaa
     1201 tcaggagccc ctagtcccag tccacaaaaa catgggagcc tggttgatga ggaagtttgg
     1261 ggtctgcctg aggagataga ggagcttagg gtgccctccc ttgtacccca ggagaggagc
     1321 attgttggcc aggaggaggc tgggacgtgg agcttgtggg ggaaggagga tgagagtctt
     1381 ctggatgagg agtttgagct tggctgggtc cagggcccag cactgactcc cgtccctgag
     1441 gaggaggaag aagaggaaga gggggctccg attgggaccc ctagggatcc tggagatggt
     1501 tgtccttccc ccgacatccc tcctgaaccc cctccaacac acctgaggcc ctgccctgcc
     1561 agccagctcc ctggactcct gtcccatggc ctcctggccg gcctctcctt tgcagtgggg
     1621 tcctcctctg gcctcctgcc cctcctgctg ctgctgctgc ttccattgct ggcagcccag
     1681 ggtgggggtg gcctgcaggc agcgctgctg gcccttgagg tggggctggt gggtctgggg
     1741 gcctcctacc tgctcctttg tacagccctg cacctgccct ccagtctttt cctactcctg
     1801 gcccagggta ccgcactggg ggccgtcctg ggcctgagct ggcgccgagg cctcatgggt
     1861 gttcccctgg gccttggagc tgcctggctc ttagcttggc caggcctagc tctacctctg
     1921 gtggctatgg cagcgggggg cagatgggtg cggcagcagg gcccccgggt gcgccggggc
     1981 atatctcgac tctggttgcg ggttctgctg cgcctgtcac ccatggcctt ccgggccctg
     2041 cagggctgtg gggctgtggg ggaccggggt ctgtttgcac tgtaccccaa aaccaacaag
     2101 gatggcttcc gcagccgcct gcccgtccct gggccccggc ggcgtaatcc ccgcaccacc
     2161 caacacccat tagctctgtt ggcaagggtc tgggtcctgt gcaagggctg gaactggcgt
     2221 ctggcacggg ccagccaggg tttagcatcc cacttgcccc cgtgggccat ccacacactg
     2281 gccagctggg gcctgcttcg gggtgaacgg cccacccgaa tcccccggct actaccacgc
     2341 agccagcgcc agctagggcc ccctgcctcc caccagccac tgccagggac tctagccggg
     2401 cggaggtcac gcacccgcca gtcccgggcc ctgcccccct ggaggtagct gactccagcc
     2461 cttccagccc aaatctagag cattgagtag atagaattcc tgcagaagct tggccgccat
     2521 ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2641 tatcttatca tgtctggatc gggaattaat tcggcgcagc accatggcct gaaataacct
     2701 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
     2761 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2821 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2881 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2941 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3001 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3061 tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg ccgtcgtttt
     3121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3181 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3241 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3301 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     3361 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3421 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3481 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3541 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3601 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3661 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3721 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3781 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3841 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3901 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3961 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4021 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6061 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6181 acaggaaaca gctatgacat gattacgaat taa
//
