LOCUS       sequence-388278-       17379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 cytosine base editing plasmid in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17379)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 17379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL670
FEATURES             Location/Qualifiers
     source          1..17379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6130..6150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6358..6423
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7027..7047
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7054..7305
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7306..7359
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7546..7566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7579..7599
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7612..7632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7643..7891
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8153..8458)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8255..8274)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8941..8965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8995..9019
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10320..10949
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11378..12451
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12517..12711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12947..12969)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13045..13185
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13100..13119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13200..13217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13371..13959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13451..13470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14046..14840)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15265..15289
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    15367..15541
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(16631..17307)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(16674..16694)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gacaaagctg
     2041 aaccagccat acggcatcgg cctcgctatt ggctcaaact ctatcggctt cgcggtggtc
     2101 gacgccaatt cccacctcct gcgcctgaag ggcgagacag ccatcggcgc caggctcttc
     2161 cgcgagggac agtcagccgc ggatcggagg ggctctagga ccacacggag gcgcctctcc
     2221 cggacaaggt ggcgcctcag cttcctgcgc gacttcttcg cgccacatat caccaagatt
     2281 gaccctgatt tcttcctccg ccagaagtac tccgagatca gccctaagga caaggatcgg
     2341 ttcaagtacg agaagaggct gttcaacgac cgcaccgatg ccgagttcta cgaggattac
     2401 ccatcaatgt accacctgag gctccatctg atgacccaca cacataaggc cgacccgagg
     2461 gagattttcc tcgccatcca ccatattctc aagtctcggg gccacttcct cacacctgga
     2521 gcggccaagg atttcaatac cgacaaggtg gatctggagg acatcttccc agccctcaca
     2581 gaggcgtacg cccaggtcta cccggacctg gagctgacct tcgacctcgc gaaggccgac
     2641 gatttcaagg cgaagctcct ggatgagcag gccaccccgt ccgacacaca gaaggcgctg
     2701 gtgaacctcc tgctctccag cgatggcgag aaggagattg tcaagaagag gaagcaggtg
     2761 ctgacagagt tcgcgaaggc catcaccggc ctcaagacaa agttcaacct cgccctgggc
     2821 accgaggtgg atgaggccga tgcctcaaat tggcagttct ctatgggcca gctcgacgat
     2881 aagtggtcca acatcgagac atccatgaca gaccagggca ccgagatttt cgagcagatt
     2941 caggagctgt accgggcgag gctgctcaac ggcattgtgc cagccggcat gtccctgagc
     3001 caggccaagg tcgccgatta cggccagcac aaggaggacc tggagctgtt caagacatac
     3061 ctcaagaagc tgaatgatca tgagctggcg aagaccatcc ggggcctcta cgacaggtac
     3121 attaacggcg acgatgccaa gccgttcctg cgcgaggact tcgtcaaggc gctcaccaag
     3181 gaggtgacag cccatccaaa cgaggtgtcc gagcagctgc tcaacaggat gggccaggcc
     3241 aatttcatgc tcaagcagag gaccaaggcc aacggcgcaa tcccaattca gctccagcag
     3301 cgcgagctgg atcagatcat tgcgaaccag agcaagtact acgactggct cgccgcccca
     3361 aatccagtgg aggcacacag gtggaagatg ccgtaccagc tcgatgagct gctcaatttc
     3421 catatcccat actacgtcgg ccctctgatt acaccgaagc agcaggcgga gtcaggcgag
     3481 aacgtgttcg cctggatggt caggaaggac ccgtctggca acatcacccc atacaatttc
     3541 gatgagaagg tggaccggga ggcgtcagcc aatacattca tccagaggat gaagaccaca
     3601 gatacctacc tgattggcga ggacgtgctc cctaagcagt ctctgctcta ccagaagtac
     3661 gaggtgctca acgagctgaa caatgtccgc atcaacaatg agtgcctggg cacagatcag
     3721 aagcagcggc tcatcaggga ggtgttcgag cggcactcat ctgtgaccat taagcaggtc
     3781 gcggataatc tcgtggcaca tggcgacttc gcccggaggc cggagatcag gggcctggcg
     3841 gacgagaaga ggttcctctc cagcctgtcc acataccacc agctcaagga gatcctgcat
     3901 gaggccattg acgatccaac caagctgctc gatatcgaga acatcattac ctggagcaca
     3961 gtgttcgagg accacaccat cttcgagaca aagctggccg agattgagtg gctcgacccg
     4021 aagaagatca acgagctgtc aggcattcgc taccggggat ggggacagtt ctctcgcaag
     4081 ctgctcgacg gcctcaagct gggcaacggc cacacagtca ttcaggagct gatgctgagc
     4141 aaccataatc tcatgcagat cctggccgat gagacactca aggagacaat gacagagctg
     4201 aaccaggaca agctcaagac agacgatatc gaggatgtga ttaacgacgc gtacacctca
     4261 ccatctaata agaaggccct ccggcaggtc ctgagggtgg tcgaggatat taagcatgcc
     4321 gcgaatggcc aggacccttc atggctcttc atcgagacag ccgacggcac cggcacagcc
     4381 ggcaagcgca cacagtctcg gcagaagcag atccagaccg tgtacgcgaa cgccgcgcag
     4441 gagctgattg attccgccgt gaggggcgag ctggaggata agatcgcgga caaggcctct
     4501 ttcacagacc gcctcgtgct gtacttcatg cagggcggca gggatatcta cacaggcgcc
     4561 cctctgaata ttgaccagct ctcccactac gacatcgatc atattctccc gcagtcactg
     4621 attaaggacg attctctgga caacagggtg ctcgtcaacg cgacaatcaa tcgcgagaag
     4681 aacaatgtgt tcgcctccac actcttcgcg ggcaagatga aggcaacctg gcggaagtgg
     4741 catgaggcag gcctgatttc aggacgcaag ctccggaatc tcatgctgag gccggatgag
     4801 atcgacaagt tcgccaaggg attcgtggca aggcagctgg tggagacaag gcagatcatt
     4861 aagctcacag agcagatcgc tgccgcccag taccctaaca ccaagatcat tgccgtgaag
     4921 gcgggcctca gccaccagct gagggaggag ctggatttcc cgaagaacag ggacgtgaat
     4981 cattaccacc atgcgttcga tgccttcctc gccgcaagga ttggcacata cctgctcaag
     5041 cgctacccga agctggcgcc tttcttcacc tacggcgagt tcgccaaggt ggacgtgaag
     5101 aagttcaggg agttcaactt catcggcgcg ctcacacacg ccaagaagaa tatcattgcg
     5161 aaggataccg gcgagattgt gtgggataag gagagggaca tccgcgagct ggacaggatc
     5221 tacaatttca agcgcatgct cattacccat gaggtctact tcgagacagc cgatctcttc
     5281 aagcagacca tctacgccgc aaaggactcc aaggagaggg gcggcagcaa gcagctgatt
     5341 cctaagaagc agggctaccc gacacaggtg tacggcggat acacccagga gtcaggcagc
     5401 tacaatgcgc tcgtgcgcgt ggccgaggca gataccacag cctaccaggt catcaagatt
     5461 tccgcgcaga acgcctccaa gatcgcgagc gccaatctca agagcaggga gaagggcaag
     5521 cagctgctca atgagattgt ggtcaagcag ctggcgaagc gccggaagaa ctggaagcct
     5581 tcagccaatt ctttcaagat cgtgattccg aggttcggca tgggcacact gttccagaac
     5641 gccaagtacg gcctcttcat ggtcaactca gacacctact accgcaatta ccaggagctg
     5701 tggctgtccc gggagaatca gaagctgctc aagaagctct tcagcatcaa gtacgagaag
     5761 acacagatga accacgatgc cctccaggtg tacaaggcca tcattgacca ggtcgagaag
     5821 ttcttcaagc tgtacgatat caatcagttc agggcgaagc tctccgacgc cattgagcgc
     5881 ttcgagaagc tgccgatcaa caccgatggc aataagattg gcaagaccga gacactccgg
     5941 cagatcctca ttggcctcca ggcgaacggc acaaggtcca acgtgaagaa tctgggcatc
     6001 aagaccgacc tcggcctgct ccaggtcggc agcggcatca agctcgacaa ggatacccag
     6061 attgtctacc agtcaccgtc tggcctgttc aagaggcgca tcccactcgc cgacctgtca
     6121 agggctgatc ctaagaagaa gaggaaggtt ggaggtggcg gtacaggagg gggtgggtcc
     6181 gctgagtatg tcagggcgtt gttcgacttc aatggaaacg acgaggaaga tctgcctttt
     6241 aaaaagggag atattctcag gatcagagat aagccggaag aacaatggtg gaacgctgaa
     6301 gactctgaag gtaagagagg tatgattctt gtcccctacg tcgagaagta ttcgggtgac
     6361 tataaagacc acgatggaga ttataaggac cacgatatag attataagga tgatgatgat
     6421 aagagcggaa tgaccgatgc agagtacgtc aggattcatg agaaacttga catctacacg
     6481 tttaagaaac agtttttcaa caacaaaaaa tctgttagtc accgctgtta cgtgctgttc
     6541 gaattgaaac gcagaggtga gaggagagcc tgcttttggg gctatgccgt caacaagccg
     6601 caaagcggca cagaaagggg cattcacgcg gagatattta gcattagaaa ggtcgaggaa
     6661 taccttcggg ataatcccgg gcaattcact atcaattggt actcttcatg gtccccgtgt
     6721 gcagattgcg ctgaaaagat actggagtgg tataatcaag aactcagagg aaacggtcac
     6781 accctcaaga tttgggcttg caagctttac tacgagaaaa atgcaaggaa ccagatcggc
     6841 ctctggaact tgcgcgacaa cggcgtgggg ttgaatgtga tggtgtcgga gcattaccag
     6901 tgctgccgga agatattcat tcagtcgtca cataatcaat tgaacgagaa taggtggctc
     6961 gaaaaaaccc tgaagcgggc cgagaagtgg aggagtgaac tctcgataat gatccaggtt
     7021 aaaggaccga agaagaagcg caaggtgggg accatgacta atctctcaga tataatcgag
     7081 aaggaaacag gaaagcaact ggtcatccaa gaatcgattt tgatgcttcc cgaagaagtc
     7141 gaagaagtta taggaaataa gcccgagtct gacatactgg ttcacacagc gtacgatgaa
     7201 agtacggacg agaatgtcat gttgctgaca tcggacgcac ctgaatacaa gccttgggct
     7261 ctggtcatac aagatagtaa cggagaaaat aagattaaaa tgcttgaagg aaggggctcc
     7321 ctgctgacat gtggagatgt ggaagagaat ccaggaccat ccaggatgat agagcaagat
     7381 ggttgtaccc aagtgctgcg gcccctcgga tggcgggggt acagcgccat gactggccat
     7441 aatagacagt ccgctgctct catgcctcca tgcagcggat gtcagaggag gggagctagg
     7501 ttcttcctgt ctagacctac ctgccccgtg ccctcaaggg ctgatcctaa gaagaagagg
     7561 aaggtttcta gggcagatcc aaagaagaag cggaaggtgt ctagagctga tccgaagaag
     7621 aagcgcaagg tgtgactcga gatatgaaga tgaagatgaa atatttggtg tgtcaaataa
     7681 aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc
     7741 tttttctaat attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata
     7801 atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg
     7861 gtatggacta tggaatatga ttaaagataa gaggatccgc ggatcatgaa ccaacggcct
     7921 ggctgtattt ggtggttgtg tagggagatg gggagaagaa aagcccgatt ctcttcgctg
     7981 tgatgggctg gatgcatgcg ggggagcggg aggcccaagt acgtgcacgg tgagcggccc
     8041 acagggcgag tgtgagcgcg agaggcggga ggaacagttt agtaccacat tgcccagcta
     8101 actcgaacgc gaccaactta taaacccgcg cgctgtcgct tgtgtggaga ccttatattc
     8161 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     8221 cttcttcccc gataacggaa accggcacac tggccatatc ggtggtcatc atgcgccagc
     8281 tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac
     8341 gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta
     8401 catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt
     8461 caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca
     8521 gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc
     8581 tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat
     8641 agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat
     8701 acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc
     8761 atactgttat ctggcttggt accggtctca gtctcaggta gatgtcagat caatcagaaa
     8821 tgattgatct gacatctacg agttgagatc aaacaaagct tcagctgagt ttcaatttct
     8881 gagcccatgt tgggccatac atatgccacc cgagtgcaaa tcgggtggct ttttttctag
     8941 acccagcttt cttgtacaaa gttggcatta ctgcgagctc aaactatcag tgtttgacag
     9001 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     9061 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     9121 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     9181 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     9241 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     9301 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     9361 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     9421 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     9481 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     9541 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     9601 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     9661 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     9721 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     9781 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     9841 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     9901 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     9961 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
    10021 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
    10081 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt gcggctgcat
    10141 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
    10201 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
    10261 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
    10321 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
    10381 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
    10441 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
    10501 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
    10561 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
    10621 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
    10681 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
    10741 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
    10801 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
    10861 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
    10921 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
    10981 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
    11041 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
    11101 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
    11161 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
    11221 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
    11281 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
    11341 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
    11401 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
    11461 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
    11521 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
    11581 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
    11641 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
    11701 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
    11761 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    11821 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    11881 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    11941 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    12001 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    12061 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    12121 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    12181 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    12241 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    12301 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    12361 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    12421 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    12481 cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt acattgggaa
    12541 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    12601 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    12661 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    12721 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    12781 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    12841 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    12901 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    12961 agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcgcgtc
    13021 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
    13081 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    13141 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
    13201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    13261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    13321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    13381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    13441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    13501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    13561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    13621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    13681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    13741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    13801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    13861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    13921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    13981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
    14041 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
    14101 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    14161 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    14221 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    14281 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    14341 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    14401 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    14461 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    14521 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
    14581 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
    14641 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    14701 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    14761 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    14821 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
    14881 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    14941 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    15001 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
    15061 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
    15121 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
    15181 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    15241 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    15301 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
    15361 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
    15421 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
    15481 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
    15541 aatagagctt gtcgatcgtt aattaatcac ctccaagatc ccggacgagt gctggggcgt
    15601 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
    15661 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
    15721 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
    15781 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
    15841 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
    15901 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
    15961 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
    16021 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
    16081 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
    16141 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
    16201 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
    16261 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
    16321 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
    16381 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
    16441 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
    16501 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
    16561 acagacgtcg cggtgagttc aggctttttc atcctaggag agatagattt gtagagagag
    16621 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
    16681 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    16741 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    16801 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    16861 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    16921 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    16981 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    17041 cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
    17101 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
    17161 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
    17221 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
    17281 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
    17341 atattcttgg agtagacgag agtgtcgtgc tccaccatg
//
