LOCUS       sequence-388280-       16561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 adenine base editing plasmid V2.0 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16561)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 16561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL673
FEATURES             Location/Qualifiers
     source          1..16561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6728..6748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6761..6781
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6794..6814
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6825..7073
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(7335..7640)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7437..7456)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8123..8147)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8177..8201
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9502..10131
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10560..11633
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11699..11893
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12129..12151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12227..12367
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12282..12301
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12382..12399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12553..13141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12633..12652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13228..14022)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14447..14471
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    14549..14723
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15813..16489)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15856..15876)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tgtctgaggt
     2041 ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacggga
     2101 tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg
     2161 ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag
     2221 acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt
     2281 cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt
     2341 tggcgtgagg aactcaaaaa gaggcgccgc aggctccctg atgaacgtgc tgaactaccc
     2401 cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct
     2461 gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag
     2521 ctccatcaac tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag
     2581 cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcaatga caaagctgaa
     2641 ccagccatac ggcatcggcc tcgctattgg ctcaaactct atcggcttcg cggtggtcga
     2701 cgccaattcc cacctcctgc gcctgaaggg cgagacagcc atcggcgcca ggctcttccg
     2761 cgagggacag tcagccgcgg atcggagggg ctctaggacc acacggaggc gcctctcccg
     2821 gacaaggtgg cgcctcagct tcctgcgcga cttcttcgcg ccacatatca ccaagattga
     2881 ccctgatttc ttcctccgcc agaagtactc cgagatcagc cctaaggaca aggatcggtt
     2941 caagtacgag aagaggctgt tcaacgaccg caccgatgcc gagttctacg aggattaccc
     3001 atcaatgtac cacctgaggc tccatctgat gacccacaca cataaggccg acccgaggga
     3061 gattttcctc gccatccacc atattctcaa gtctcggggc cacttcctca cacctggagc
     3121 ggccaaggat ttcaataccg acaaggtgga tctggaggac atcttcccag ccctcacaga
     3181 ggcgtacgcc caggtctacc cggacctgga gctgaccttc gacctcgcga aggccgacga
     3241 tttcaaggcg aagctcctgg atgagcaggc caccccgtcc gacacacaga aggcgctggt
     3301 gaacctcctg ctctccagcg atggcgagaa ggagattgtc aagaagagga agcaggtgct
     3361 gacagagttc gcgaaggcca tcaccggcct caagacaaag ttcaacctcg ccctgggcac
     3421 cgaggtggat gaggccgatg cctcaaattg gcagttctct atgggccagc tcgacgataa
     3481 gtggtccaac atcgagacat ccatgacaga ccagggcacc gagattttcg agcagattca
     3541 ggagctgtac cgggcgaggc tgctcaacgg cattgtgcca gccggcatgt ccctgagcca
     3601 ggccaaggtc gccgattacg gccagcacaa ggaggacctg gagctgttca agacatacct
     3661 caagaagctg aatgatcatg agctggcgaa gaccatccgg ggcctctacg acaggtacat
     3721 taacggcgac gatgccaagc cgttcctgcg cgaggacttc gtcaaggcgc tcaccaagga
     3781 ggtgacagcc catccaaacg aggtgtccga gcagctgctc aacaggatgg gccaggccaa
     3841 tttcatgctc aagcagagga ccaaggccaa cggcgcaatc ccaattcagc tccagcagcg
     3901 cgagctggat cagatcattg cgaaccagag caagtactac gactggctcg ccgccccaaa
     3961 tccagtggag gcacacaggt ggaagatgcc gtaccagctc gatgagctgc tcaatttcca
     4021 tatcccatac tacgtcggcc ctctgattac accgaagcag caggcggagt caggcgagaa
     4081 cgtgttcgcc tggatggtca ggaaggaccc gtctggcaac atcaccccat acaatttcga
     4141 tgagaaggtg gaccgggagg cgtcagccaa tacattcatc cagaggatga agaccacaga
     4201 tacctacctg attggcgagg acgtgctccc taagcagtct ctgctctacc agaagtacga
     4261 ggtgctcaac gagctgaaca atgtccgcat caacaatgag tgcctgggca cagatcagaa
     4321 gcagcggctc atcagggagg tgttcgagcg gcactcatct gtgaccatta agcaggtcgc
     4381 ggataatctc gtggcacatg gcgacttcgc ccggaggccg gagatcaggg gcctggcgga
     4441 cgagaagagg ttcctctcca gcctgtccac ataccaccag ctcaaggaga tcctgcatga
     4501 ggccattgac gatccaacca agctgctcga tatcgagaac atcattacct ggagcacagt
     4561 gttcgaggac cacaccatct tcgagacaaa gctggccgag attgagtggc tcgacccgaa
     4621 gaagatcaac gagctgtcag gcattcgcta ccggggatgg ggacagttct ctcgcaagct
     4681 gctcgacggc ctcaagctgg gcaacggcca cacagtcatt caggagctga tgctgagcaa
     4741 ccataatctc atgcagatcc tggccgatga gacactcaag gagacaatga cagagctgaa
     4801 ccaggacaag ctcaagacag acgatatcga ggatgtgatt aacgacgcgt acacctcacc
     4861 atctaataag aaggccctcc ggcaggtcct gagggtggtc gaggatatta agcatgccgc
     4921 gaatggccag gacccttcat ggctcttcat cgagacagcc gacggcaccg gcacagccgg
     4981 caagcgcaca cagtctcggc agaagcagat ccagaccgtg tacgcgaacg ccgcgcagga
     5041 gctgattgat tccgccgtga ggggcgagct ggaggataag atcgcggaca aggcctcttt
     5101 cacagaccgc ctcgtgctgt acttcatgca gggcggcagg gatatctaca caggcgcccc
     5161 tctgaatatt gaccagctct cccactacga catcgatcat attctcccgc agtcactgat
     5221 taaggacgat tctctggaca acagggtgct cgtcaacgcg acaatcaatc gcgagaagaa
     5281 caatgtgttc gcctccacac tcttcgcggg caagatgaag gcaacctggc ggaagtggca
     5341 tgaggcaggc ctgatttcag gacgcaagct ccggaatctc atgctgaggc cggatgagat
     5401 cgacaagttc gccaagggat tcgtggcaag gcagctggtg gagacaaggc agatcattaa
     5461 gctcacagag cagatcgctg ccgcccagta ccctaacacc aagatcattg ccgtgaaggc
     5521 gggcctcagc caccagctga gggaggagct ggatttcccg aagaacaggg acgtgaatca
     5581 ttaccaccat gcgttcgatg ccttcctcgc cgcaaggatt ggcacatacc tgctcaagcg
     5641 ctacccgaag ctggcgcctt tcttcaccta cggcgagttc gccaaggtgg acgtgaagaa
     5701 gttcagggag ttcaacttca tcggcgcgct cacacacgcc aagaagaata tcattgcgaa
     5761 ggataccggc gagattgtgt gggataagga gagggacatc cgcgagctgg acaggatcta
     5821 caatttcaag cgcatgctca ttacccatga ggtctacttc gagacagccg atctcttcaa
     5881 gcagaccatc tacgccgcaa aggactccaa ggagaggggc ggcagcaagc agctgattcc
     5941 taagaagcag ggctacccga cacaggtgta cggcggatac acccaggagt caggcagcta
     6001 caatgcgctc gtgcgcgtgg ccgaggcaga taccacagcc taccaggtca tcaagatttc
     6061 cgcgcagaac gcctccaaga tcgcgagcgc caatctcaag agcagggaga agggcaagca
     6121 gctgctcaat gagattgtgg tcaagcagct ggcgaagcgc cggaagaact ggaagccttc
     6181 agccaattct ttcaagatcg tgattccgag gttcggcatg ggcacactgt tccagaacgc
     6241 caagtacggc ctcttcatgg tcaactcaga cacctactac cgcaattacc aggagctgtg
     6301 gctgtcccgg gagaatcaga agctgctcaa gaagctcttc agcatcaagt acgagaagac
     6361 acagatgaac cacgatgccc tccaggtgta caaggccatc attgaccagg tcgagaagtt
     6421 cttcaagctg tacgatatca atcagttcag ggcgaagctc tccgacgcca ttgagcgctt
     6481 cgagaagctg ccgatcaaca ccgatggcaa taagattggc aagaccgaga cactccggca
     6541 gatcctcatt ggcctccagg cgaacggcac aaggtccaac gtgaagaatc tgggcatcaa
     6601 gaccgacctc ggcctgctcc aggtcggcag cggcatcaag ctcgacaagg atacccagat
     6661 tgtctaccag tcaccgtctg gcctgttcaa gaggcgcatc ccactcgccg acctgtcaag
     6721 ggctgatcct aagaagaaga ggaaggtttc tagggcagat ccaaagaaga agcggaaggt
     6781 gtctagagct gatccgaaga agaagcgcaa ggtgtgactc gagatatgaa gatgaagatg
     6841 aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct
     6901 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa
     6961 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     7021 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagaggatcc
     7081 gcggatcatg aaccaacggc ctggctgtat ttggtggttg tgtagggaga tggggagaag
     7141 aaaagcccga ttctcttcgc tgtgatgggc tggatgcatg cgggggagcg ggaggcccaa
     7201 gtacgtgcac ggtgagcggc ccacagggcg agtgtgagcg cgagaggcgg gaggaacagt
     7261 ttagtaccac attgcccagc taactcgaac gcgaccaact tataaacccg cgcgctgtcg
     7321 cttgtgtgga gaccttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     7381 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata
     7441 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     7501 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
     7561 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     7621 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
     7681 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
     7741 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
     7801 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
     7861 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
     7921 aaaaatcagc gcgcaaatac gcatactgtt atctggcttg gtaccggtct cagtctcagg
     7981 tagatgtcag atcaatcaga aatgattgat ctgacatcta cgagttgaga tcaaacaaag
     8041 cttcagctga gtttcaattt ctgagcccat gttgggccat acatatgcca cccgagtgca
     8101 aatcgggtgg ctttttttct agacccagct ttcttgtaca aagttggcat tactgcgagc
     8161 tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     8221 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     8281 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     8341 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     8401 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     8461 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     8521 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     8581 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     8641 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     8701 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     8761 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     8821 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     8881 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     8941 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     9001 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     9061 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     9121 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     9181 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     9241 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     9301 cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     9361 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     9421 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     9481 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     9541 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     9601 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     9661 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     9721 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     9781 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     9841 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     9901 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     9961 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
    10021 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
    10081 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
    10141 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
    10201 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
    10261 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
    10321 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
    10381 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
    10441 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
    10501 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
    10561 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
    10621 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
    10681 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
    10741 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
    10801 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
    10861 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
    10921 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
    10981 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
    11041 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
    11101 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
    11161 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
    11221 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
    11281 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
    11341 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
    11401 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
    11461 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
    11521 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
    11581 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
    11641 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg
    11701 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
    11761 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
    11821 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
    11881 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
    11941 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
    12001 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
    12061 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
    12121 ggtgaaaacc tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat
    12181 gccgggagca gacaagcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
    12241 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
    12301 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    12361 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12961 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    13021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    13081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    13141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    13201 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    13261 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    13321 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    13381 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    13441 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    13501 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    13561 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    13621 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    13681 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    13741 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    13801 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    13861 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    13921 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    13981 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    14041 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    14101 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    14161 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    14221 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    14281 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    14341 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    14401 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    14461 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    14521 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    14581 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    14641 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    14701 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
    14761 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
    14821 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
    14881 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
    14941 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
    15001 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
    15061 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
    15121 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
    15181 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
    15241 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
    15301 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
    15361 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
    15421 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
    15481 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
    15541 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
    15601 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
    15661 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
    15721 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
    15781 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
    15841 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
    15901 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
    15961 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
    16021 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
    16081 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
    16141 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
    16201 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
    16261 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
    16321 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
    16381 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
    16441 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
    16501 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    16561 g
//
