LOCUS       sequence-388284-       16081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LrCas9 genome editing plasmid in wheat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu 
            S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y
  TITLE     Efficient plant genome engineering using a probiotic sourced 
            CRISPR-Cas9 system.
  JOURNAL   Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9.
  PUBMED    37773156
REFERENCE   2  (bases 1 to 16081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-29"; volume: "14(1):6102. doi"; pages: " 
            10.1038/s41467-023-41802-9"
COMMENT     Sequence Label: pGEL667
FEATURES             Location/Qualifiers
     source          1..16081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             6130..6150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6163..6183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6196..6216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6227..6475
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(6855..7160)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6957..6976)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7643..7667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7697..7721
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9022..9651
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10080..11153
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11219..11413
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11649..11671)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11747..11887
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11802..11821
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11902..11919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12073..12661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12153..12172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12748..13542)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13967..13991
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    14069..14243
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15333..16009)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15376..15396)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat gacaaagctg
     2041 aaccagccat acggcatcgg cctcgatatt ggctcaaact ctatcggctt cgcggtggtc
     2101 gacgccaatt cccacctcct gcgcctgaag ggcgagacag ccatcggcgc caggctcttc
     2161 cgcgagggac agtcagccgc ggatcggagg ggctctagga ccacacggag gcgcctctcc
     2221 cggacaaggt ggcgcctcag cttcctgcgc gacttcttcg cgccacatat caccaagatt
     2281 gaccctgatt tcttcctccg ccagaagtac tccgagatca gccctaagga caaggatcgg
     2341 ttcaagtacg agaagaggct gttcaacgac cgcaccgatg ccgagttcta cgaggattac
     2401 ccatcaatgt accacctgag gctccatctg atgacccaca cacataaggc cgacccgagg
     2461 gagattttcc tcgccatcca ccatattctc aagtctcggg gccacttcct cacacctgga
     2521 gcggccaagg atttcaatac cgacaaggtg gatctggagg acatcttccc agccctcaca
     2581 gaggcgtacg cccaggtcta cccggacctg gagctgacct tcgacctcgc gaaggccgac
     2641 gatttcaagg cgaagctcct ggatgagcag gccaccccgt ccgacacaca gaaggcgctg
     2701 gtgaacctcc tgctctccag cgatggcgag aaggagattg tcaagaagag gaagcaggtg
     2761 ctgacagagt tcgcgaaggc catcaccggc ctcaagacaa agttcaacct cgccctgggc
     2821 accgaggtgg atgaggccga tgcctcaaat tggcagttct ctatgggcca gctcgacgat
     2881 aagtggtcca acatcgagac atccatgaca gaccagggca ccgagatttt cgagcagatt
     2941 caggagctgt accgggcgag gctgctcaac ggcattgtgc cagccggcat gtccctgagc
     3001 caggccaagg tcgccgatta cggccagcac aaggaggacc tggagctgtt caagacatac
     3061 ctcaagaagc tgaatgatca tgagctggcg aagaccatcc ggggcctcta cgacaggtac
     3121 attaacggcg acgatgccaa gccgttcctg cgcgaggact tcgtcaaggc gctcaccaag
     3181 gaggtgacag cccatccaaa cgaggtgtcc gagcagctgc tcaacaggat gggccaggcc
     3241 aatttcatgc tcaagcagag gaccaaggcc aacggcgcaa tcccaattca gctccagcag
     3301 cgcgagctgg atcagatcat tgcgaaccag agcaagtact acgactggct cgccgcccca
     3361 aatccagtgg aggcacacag gtggaagatg ccgtaccagc tcgatgagct gctcaatttc
     3421 catatcccat actacgtcgg ccctctgatt acaccgaagc agcaggcgga gtcaggcgag
     3481 aacgtgttcg cctggatggt caggaaggac ccgtctggca acatcacccc atacaatttc
     3541 gatgagaagg tggaccggga ggcgtcagcc aatacattca tccagaggat gaagaccaca
     3601 gatacctacc tgattggcga ggacgtgctc cctaagcagt ctctgctcta ccagaagtac
     3661 gaggtgctca acgagctgaa caatgtccgc atcaacaatg agtgcctggg cacagatcag
     3721 aagcagcggc tcatcaggga ggtgttcgag cggcactcat ctgtgaccat taagcaggtc
     3781 gcggataatc tcgtggcaca tggcgacttc gcccggaggc cggagatcag gggcctggcg
     3841 gacgagaaga ggttcctctc cagcctgtcc acataccacc agctcaagga gatcctgcat
     3901 gaggccattg acgatccaac caagctgctc gatatcgaga acatcattac ctggagcaca
     3961 gtgttcgagg accacaccat cttcgagaca aagctggccg agattgagtg gctcgacccg
     4021 aagaagatca acgagctgtc aggcattcgc taccggggat ggggacagtt ctctcgcaag
     4081 ctgctcgacg gcctcaagct gggcaacggc cacacagtca ttcaggagct gatgctgagc
     4141 aaccataatc tcatgcagat cctggccgat gagacactca aggagacaat gacagagctg
     4201 aaccaggaca agctcaagac agacgatatc gaggatgtga ttaacgacgc gtacacctca
     4261 ccatctaata agaaggccct ccggcaggtc ctgagggtgg tcgaggatat taagcatgcc
     4321 gcgaatggcc aggacccttc atggctcttc atcgagacag ccgacggcac cggcacagcc
     4381 ggcaagcgca cacagtctcg gcagaagcag atccagaccg tgtacgcgaa cgccgcgcag
     4441 gagctgattg attccgccgt gaggggcgag ctggaggata agatcgcgga caaggcctct
     4501 ttcacagacc gcctcgtgct gtacttcatg cagggcggca gggatatcta cacaggcgcc
     4561 cctctgaata ttgaccagct ctcccactac gacatcgatc atattctccc gcagtcactg
     4621 attaaggacg attctctgga caacagggtg ctcgtcaacg cgacaatcaa tcgcgagaag
     4681 aacaatgtgt tcgcctccac actcttcgcg ggcaagatga aggcaacctg gcggaagtgg
     4741 catgaggcag gcctgatttc aggacgcaag ctccggaatc tcatgctgag gccggatgag
     4801 atcgacaagt tcgccaaggg attcgtggca aggcagctgg tggagacaag gcagatcatt
     4861 aagctcacag agcagatcgc tgccgcccag taccctaaca ccaagatcat tgccgtgaag
     4921 gcgggcctca gccaccagct gagggaggag ctggatttcc cgaagaacag ggacgtgaat
     4981 cattaccacc atgcgttcga tgccttcctc gccgcaagga ttggcacata cctgctcaag
     5041 cgctacccga agctggcgcc tttcttcacc tacggcgagt tcgccaaggt ggacgtgaag
     5101 aagttcaggg agttcaactt catcggcgcg ctcacacacg ccaagaagaa tatcattgcg
     5161 aaggataccg gcgagattgt gtgggataag gagagggaca tccgcgagct ggacaggatc
     5221 tacaatttca agcgcatgct cattacccat gaggtctact tcgagacagc cgatctcttc
     5281 aagcagacca tctacgccgc aaaggactcc aaggagaggg gcggcagcaa gcagctgatt
     5341 cctaagaagc agggctaccc gacacaggtg tacggcggat acacccagga gtcaggcagc
     5401 tacaatgcgc tcgtgcgcgt ggccgaggca gataccacag cctaccaggt catcaagatt
     5461 tccgcgcaga acgcctccaa gatcgcgagc gccaatctca agagcaggga gaagggcaag
     5521 cagctgctca atgagattgt ggtcaagcag ctggcgaagc gccggaagaa ctggaagcct
     5581 tcagccaatt ctttcaagat cgtgattccg aggttcggca tgggcacact gttccagaac
     5641 gccaagtacg gcctcttcat ggtcaactca gacacctact accgcaatta ccaggagctg
     5701 tggctgtccc gggagaatca gaagctgctc aagaagctct tcagcatcaa gtacgagaag
     5761 acacagatga accacgatgc cctccaggtg tacaaggcca tcattgacca ggtcgagaag
     5821 ttcttcaagc tgtacgatat caatcagttc agggcgaagc tctccgacgc cattgagcgc
     5881 ttcgagaagc tgccgatcaa caccgatggc aataagattg gcaagaccga gacactccgg
     5941 cagatcctca ttggcctcca ggcgaacggc acaaggtcca acgtgaagaa tctgggcatc
     6001 aagaccgacc tcggcctgct ccaggtcggc agcggcatca agctcgacaa ggatacccag
     6061 attgtctacc agtcaccgtc tggcctgttc aagaggcgca tcccactcgc cgacctgtca
     6121 agggctgatc ctaagaagaa gaggaaggtt tctagggcag atccaaagaa gaagcggaag
     6181 gtgtctagag ctgatccgaa gaagaagcgc aaggtgtgac tcgagatatg aagatgaaga
     6241 tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg
     6301 cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag atcacattat
     6361 aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag
     6421 catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag ataagaggat
     6481 ccgcgaccaa gcccgttatt ctgacagttc tggtgctcaa cacatttata tttatcaagg
     6541 agcacattgt tactcactgc taggagggaa tcgaactagg aatattgatc agaggaacta
     6601 cgagagagct gaagataact gccctctagc tctcactgat ctgggtcgca tagtgagatg
     6661 cagcccacgt gagttcagca acggtctagc gctgggcttt taggcccgca tgatcgggct
     6721 tttgtcgggt ggtcgacgtg ttcacgattg gggagagcaa cgcagcagtt cctcttagtt
     6781 tagtcccacc tcgcctgtcc agcagagttc tgaccggttt ataaactcgc ttgctgcatc
     6841 agacttggga gaccttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     6901 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata
     6961 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
     7021 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
     7081 gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag
     7141 gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat
     7201 ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc
     7261 acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc
     7321 atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc
     7381 atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc
     7441 aaaaatcagc gcgcaaatac gcatactgtt atctggcttg gtaccggtct cagtctcagg
     7501 tagatgtcag atcaatcaga aatgattgat ctgacatcta cgagttgaga tcaaacaaag
     7561 cttcagctga gtttcaattt ctgagcccat gttgggccat acatatgcca cccgagtgca
     7621 aatcgggtgg ctttttttct agacccagct ttcttgtaca aagttggcat tactgcgagc
     7681 tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     7741 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     7801 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     7861 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     7921 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     7981 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     8041 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     8101 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     8161 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     8221 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     8281 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     8341 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     8401 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     8461 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     8521 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     8581 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     8641 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     8701 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     8761 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     8821 cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     8881 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     8941 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     9001 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     9061 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     9121 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     9181 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     9241 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     9301 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     9361 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     9421 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     9481 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     9541 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     9601 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     9661 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     9721 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     9781 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     9841 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     9901 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     9961 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
    10021 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
    10081 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
    10141 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
    10201 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
    10261 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
    10321 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
    10381 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
    10441 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
    10501 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
    10561 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
    10621 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
    10681 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
    10741 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
    10801 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
    10861 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
    10921 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
    10981 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
    11041 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
    11101 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
    11161 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg
    11221 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
    11281 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
    11341 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
    11401 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
    11461 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
    11521 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
    11581 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
    11641 ggtgaaaacc tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat
    11701 gccgggagca gacaagcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
    11761 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
    11821 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    11881 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    11941 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12001 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12061 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12121 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12181 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12241 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12301 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12361 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12421 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12481 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    12541 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12601 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12661 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12721 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    12781 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    12841 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    12901 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    12961 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    13021 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    13081 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    13141 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    13201 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    13261 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    13321 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    13381 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    13441 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    13501 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    13561 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    13621 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    13681 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    13741 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    13801 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    13861 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    13921 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    13981 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    14041 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    14101 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    14161 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    14221 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
    14281 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
    14341 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
    14401 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
    14461 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
    14521 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
    14581 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
    14641 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
    14701 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
    14761 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
    14821 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
    14881 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
    14941 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
    15001 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
    15061 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
    15121 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
    15181 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
    15241 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
    15301 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
    15361 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
    15421 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
    15481 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
    15541 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
    15601 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
    15661 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
    15721 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
    15781 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
    15841 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
    15901 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
    15961 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
    16021 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    16081 g
//
